Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   RCG20_RS06535 Genome accession   NZ_CP133266
Coordinates   1265547..1266587 (-) Length   346 a.a.
NCBI ID   WP_308183428.1    Uniprot ID   -
Organism   Neobacillus sp. PS3-40     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1260547..1271587
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RCG20_RS06510 (RCG20_06510) - 1261269..1262015 (-) 747 WP_308183423.1 hypothetical protein -
  RCG20_RS06515 (RCG20_06515) pilM 1262012..1263007 (-) 996 WP_308183424.1 pilus assembly protein PilM -
  RCG20_RS06520 (RCG20_06520) - 1263024..1263788 (-) 765 WP_308183425.1 prepilin peptidase -
  RCG20_RS06525 (RCG20_06525) - 1263778..1264194 (-) 417 WP_308183426.1 type II secretion system protein -
  RCG20_RS06530 (RCG20_06530) - 1264331..1265545 (-) 1215 WP_308183427.1 type II secretion system F family protein -
  RCG20_RS06535 (RCG20_06535) pilT 1265547..1266587 (-) 1041 WP_308183428.1 type IV pilus twitching motility protein PilT Machinery gene
  RCG20_RS06540 (RCG20_06540) - 1266601..1268265 (-) 1665 WP_308183429.1 ATPase, T2SS/T4P/T4SS family -
  RCG20_RS06545 (RCG20_06545) - 1268300..1269316 (-) 1017 WP_308183430.1 hypothetical protein -
  RCG20_RS06550 (RCG20_06550) - 1269359..1270168 (-) 810 WP_308183431.1 hypothetical protein -
  RCG20_RS06555 (RCG20_06555) - 1270195..1271121 (-) 927 WP_308183432.1 hypothetical protein -
  RCG20_RS06560 (RCG20_06560) - 1271124..1271540 (-) 417 WP_308183433.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -

Sequence

Protein


Download         Length: 346 a.a.        Molecular weight: 38615.96 Da        Isoelectric Point: 7.2789

>NTDB_id=873005 RCG20_RS06535 WP_308183428.1 1265547..1266587(-) (pilT) [Neobacillus sp. PS3-40]
MKDKIDLLMRAAYELKASDIHLTVGVPPVMRINGELKRYGKEILVPADTEGMARAIVPSQMWDLFKEKGELDFSYGLPGV
SRFRINTYHQRACVSMAIRIVPSKIPSIEELDLPETLTKIIEKPQGLILVTGPTGSGKSTTLAAMIHYMNQYMKKHIITL
EDPIEYLHKHGNSIIDQREVGFDTKNFANGLRAALRQDPDVILVGEMRDLETIQTAITAAETGHLVLGTLHTSSAPATIN
RIIDVFEPAQQPQIRIQLASVLVGIISQRLFPTIDRKGRKAATEIMINNAAIANLIRNEKIHQIISIMQTSRAQGMHTLE
SSIKDLIEDGFIQKELALPFLQEQLI

Nucleotide


Download         Length: 1041 bp        

>NTDB_id=873005 RCG20_RS06535 WP_308183428.1 1265547..1266587(-) (pilT) [Neobacillus sp. PS3-40]
ATGAAAGATAAAATCGATCTTTTAATGCGAGCTGCTTATGAATTAAAGGCATCTGATATCCATTTAACAGTAGGTGTCCC
ACCCGTCATGCGAATAAACGGGGAACTAAAAAGGTATGGAAAAGAGATTTTAGTGCCTGCAGATACAGAGGGAATGGCGA
GAGCAATCGTACCTTCGCAAATGTGGGATTTGTTTAAAGAAAAGGGAGAACTAGATTTTTCCTATGGTCTTCCTGGTGTA
TCTCGTTTTCGGATAAATACTTACCATCAACGGGCATGTGTTTCAATGGCCATTCGAATTGTCCCTTCCAAAATCCCATC
GATTGAGGAATTGGATTTACCTGAAACATTAACAAAAATCATTGAGAAGCCACAAGGTCTTATCCTTGTGACAGGACCAA
CTGGAAGCGGAAAATCAACAACACTCGCTGCCATGATCCATTATATGAATCAATATATGAAGAAGCATATTATTACGCTG
GAAGATCCAATAGAGTACCTCCATAAACATGGAAATTCGATTATCGATCAACGTGAAGTAGGTTTTGATACAAAGAATTT
TGCCAATGGATTAAGGGCCGCACTCCGCCAAGATCCCGATGTTATTCTTGTTGGAGAGATGCGTGATTTAGAAACGATTC
AAACTGCTATCACCGCAGCTGAAACAGGACATCTCGTCTTAGGTACACTTCATACTTCGAGTGCACCTGCAACAATCAAT
CGAATTATTGATGTATTTGAACCTGCACAACAGCCGCAAATTCGGATTCAGCTAGCTTCTGTTTTAGTCGGTATCATCTC
GCAGCGCTTGTTTCCAACTATTGATCGAAAAGGAAGAAAGGCTGCAACGGAAATTATGATCAATAATGCTGCTATTGCCA
ATTTGATCCGCAATGAAAAAATCCATCAGATTATAAGTATTATGCAAACGTCCCGTGCCCAAGGAATGCATACACTCGAA
TCGAGTATAAAGGATTTAATAGAAGATGGTTTTATTCAAAAAGAACTTGCATTACCATTTTTACAGGAGCAATTAATCTA
G

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(94-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.118

98.266

0.483
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.39

94.798

0.468
  pilT Vibrio cholerae strain A1552

49.39

94.798

0.468
  pilT Neisseria meningitidis 8013

47.59

95.954

0.457
  pilT Acinetobacter baumannii D1279779

46.866

96.821

0.454
  pilT Acinetobacter nosocomialis M2

46.866

96.821

0.454
  pilT Acinetobacter baumannii strain A118

46.866

96.821

0.454
  pilT Pseudomonas stutzeri DSM 10701

48.308

93.931

0.454
  pilT Neisseria gonorrhoeae MS11

47.289

95.954

0.454
  pilT Acinetobacter baylyi ADP1

46.933

94.22

0.442
  pilT Pseudomonas aeruginosa PAK

47.664

92.775

0.442
  pilT Legionella pneumophila strain Lp02

45.652

93.064

0.425
  pilT Legionella pneumophila strain ERS1305867

45.652

93.064

0.425
  pilU Pseudomonas stutzeri DSM 10701

41.692

95.665

0.399
  pilU Acinetobacter baylyi ADP1

41.049

93.642

0.384
  pilU Vibrio cholerae strain A1552

40.557

93.353

0.379