Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   RBI06_RS24495 Genome accession   NZ_CP133093
Coordinates   5304722..5305939 (+) Length   405 a.a.
NCBI ID   WP_016254080.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain 002     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5299722..5310939
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RBI06_RS24470 (RBI06_24470) nadC 5300475..5301323 (+) 849 WP_019372158.1 carboxylating nicotinate-nucleotide diphosphorylase -
  RBI06_RS24480 (RBI06_24480) - 5301486..5302184 (-) 699 WP_016254081.1 hypothetical protein -
  RBI06_RS24485 (RBI06_24485) - 5302247..5302786 (-) 540 WP_003141352.1 pilin -
  RBI06_RS24490 (RBI06_24490) pilB 5303015..5304718 (+) 1704 WP_003141354.1 type IV-A pilus assembly ATPase PilB Machinery gene
  RBI06_RS24495 (RBI06_24495) pilC 5304722..5305939 (+) 1218 WP_016254080.1 type II secretion system F family protein Machinery gene
  RBI06_RS24500 (RBI06_24500) pilD 5305942..5306814 (+) 873 WP_034007824.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  RBI06_RS24505 (RBI06_24505) coaE 5306811..5307422 (+) 612 WP_033989050.1 dephospho-CoA kinase -
  RBI06_RS24510 (RBI06_24510) yacG 5307419..5307619 (+) 201 WP_003094656.1 DNA gyrase inhibitor YacG -
  RBI06_RS24515 (RBI06_24515) - 5307656..5307865 (-) 210 WP_003094660.1 hypothetical protein -
  RBI06_RS24520 (RBI06_24520) - 5307971..5308660 (-) 690 WP_003094662.1 energy-coupling factor ABC transporter permease -
  RBI06_RS24525 (RBI06_24525) - 5308657..5309127 (-) 471 WP_003094664.1 hypothetical protein -
  RBI06_RS24530 (RBI06_24530) - 5309124..5309549 (-) 426 WP_021264128.1 GNAT family N-acetyltransferase -
  RBI06_RS24535 (RBI06_24535) - 5309682..5310311 (+) 630 WP_003094668.1 DUF1780 domain-containing protein -
  RBI06_RS24540 (RBI06_24540) - 5310308..5310757 (+) 450 WP_003094670.1 MOSC domain-containing protein -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 44041.92 Da        Isoelectric Point: 9.8417

>NTDB_id=871849 RBI06_RS24495 WP_016254080.1 5304722..5305939(+) (pilC) [Pseudomonas aeruginosa strain 002]
MADKALKTSVFIWEGTDKKGSKVKGELAGQNPMLVKAQLRKQGINPLKVRKKGITLFGAGKKIKPMDIALFTRQMSTMMG
AGVPLLQSFDIIGEGFENPNMRKLVDEIKQDVAAGNSLASSLRKKPIYFDDLYCNLVDAGEQSGALETLLDRVATYKEKT
ESLKAKIKKAMTYPIAVIVVALVVSAILLIKVVPQFQSVFANFGAELPAFTQMVINLSEMLQEWWLIVLIGLFAAAFAFR
EAHRRSQKLRDSVDRGLLKLPIIGGILYKSAIARYARTLSTTFAAGVPLVEALDSVSGATGNVVFKNAVTKIKQDVSSGM
QLNFSMRTTNVFPSMAIQMAAIGEESGSLDEMLGKVAGFYEEEVDNAVDNLTTLMEPMIMAVLGVLVGGLIIAMYLPIFQ
LGSVV

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=871849 RBI06_RS24495 WP_016254080.1 5304722..5305939(+) (pilC) [Pseudomonas aeruginosa strain 002]
ATGGCGGACAAAGCGTTAAAGACCAGTGTCTTCATCTGGGAAGGCACTGATAAGAAAGGCAGCAAGGTCAAAGGAGAGTT
GGCCGGGCAAAACCCTATGCTGGTGAAGGCTCAACTGCGCAAACAGGGTATCAACCCACTAAAGGTCCGCAAGAAAGGTA
TCACCCTGTTTGGGGCAGGGAAGAAGATTAAGCCCATGGACATCGCCCTGTTCACTCGGCAGATGTCTACCATGATGGGT
GCCGGCGTACCGTTACTGCAATCTTTTGACATCATCGGCGAAGGATTCGAAAATCCAAACATGCGCAAGCTAGTCGATGA
GATCAAGCAGGATGTTGCCGCCGGTAACAGCTTAGCCAGTTCACTTCGAAAGAAACCCATTTACTTCGATGATCTCTACT
GCAACCTGGTCGATGCTGGCGAACAGTCCGGTGCTTTGGAGACATTATTGGATCGGGTAGCAACTTATAAAGAAAAGACA
GAATCCCTGAAAGCCAAAATTAAAAAAGCCATGACTTATCCCATTGCAGTAATTGTAGTGGCCCTTGTGGTATCGGCGAT
CCTTCTGATAAAAGTGGTCCCACAGTTCCAGTCCGTATTTGCAAATTTTGGTGCCGAGTTGCCGGCCTTTACTCAAATGG
TCATCAATCTTTCCGAGATGCTTCAAGAGTGGTGGCTCATAGTGCTTATTGGTCTTTTTGCCGCAGCTTTTGCATTTAGG
GAAGCTCATCGGCGCTCGCAAAAACTGAGAGATTCAGTAGATCGGGGCCTGCTGAAACTACCTATCATCGGCGGGATACT
TTACAAATCAGCTATCGCCCGCTACGCCCGAACGCTATCCACTACCTTTGCGGCTGGAGTGCCTCTGGTAGAAGCTCTGG
ACTCCGTTTCCGGAGCAACTGGCAACGTGGTATTCAAGAATGCCGTTACCAAGATCAAACAAGACGTGTCCAGCGGCATG
CAATTGAACTTTTCCATGCGCACCACCAATGTTTTCCCCAGTATGGCTATCCAGATGGCTGCAATTGGCGAGGAATCAGG
TTCGCTAGATGAGATGTTAGGAAAAGTTGCCGGTTTCTATGAGGAAGAAGTCGATAACGCCGTCGACAACCTGACAACGT
TGATGGAGCCGATGATCATGGCCGTGCTCGGCGTACTGGTCGGCGGCCTGATCATCGCTATGTATCTACCGATCTTCCAA
CTGGGTTCCGTTGTGTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(276-397)

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

79.753

100

0.798
  pilC Acinetobacter baumannii D1279779

60.891

99.753

0.607
  pilC Acinetobacter baylyi ADP1

59.852

100

0.6
  pilC Legionella pneumophila strain ERS1305867

55.303

97.778

0.541
  pilG Neisseria meningitidis 44/76-A

46.269

99.259

0.459
  pilG Neisseria gonorrhoeae MS11

46.02

99.259

0.457
  pilC Vibrio cholerae strain A1552

42.222

100

0.422
  pilC Vibrio campbellii strain DS40M4

40.909

97.778

0.4
  pilC Thermus thermophilus HB27

38.404

99.012

0.38