Detailed information NTG0871553

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: RBI12_RS14290
• Genome accession: NZ_CP133054
• Coordinates: 2972943..2974061 (+)
• Length: 372 a.a.
• NCBI ID: WP_005803129.1
• Uniprot ID: A0A009MM04
• Organism: Acinetobacter pittii strain A774
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2967943..2979061

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
RBI12_RS14280 (RBI12_14340)	yggS	2971067..2971759 (-)	693	WP_005803127.1	YggS family pyridoxal phosphate-dependent enzyme	-
RBI12_RS14285 (RBI12_14345)	pilT	2971886..2972923 (+)	1038	WP_002121749.1	type IV pilus twitching motility protein PilT	Machinery gene
RBI12_RS14290 (RBI12_14350)	pilU	2972943..2974061 (+)	1119	WP_005803129.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
RBI12_RS14295 (RBI12_14355)	fur	2974131..2974568 (-)	438	WP_002121758.1	ferric iron uptake transcriptional regulator	-
RBI12_RS14300 (RBI12_14360)	omlA	2974680..2975078 (+)	399	WP_002121740.1	outer membrane protein assembly factor BamE	-
RBI12_RS14305 (RBI12_14365)	-	2975143..2975457 (-)	315	WP_017481335.1	RnfH family protein	-
RBI12_RS14310 (RBI12_14370)	-	2975457..2976524 (-)	1068	WP_031944146.1	membrane protein	-
RBI12_RS14315 (RBI12_14375)	-	2976678..2977064 (-)	387	Protein_2805	bacteriohemerythrin	-
RBI12_RS14320 (RBI12_14380)	-	2977072..2978040 (-)	969	WP_005804963.1	IS30 family transposase	-
RBI12_RS14325 (RBI12_14385)	-	2978152..2978223 (-)	72	Protein_2807	hemerythrin	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41903.09 Da
• Isoelectric Point: 6.6403

>NTDB_id=871553 RBI12_RS14290 WP_005803129.1 2972943..2974061(+) (pilU) [Acinetobacter pittii strain A774]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEIIGQLLHSIMSEKQRKEFAETRECNFAIMNRDKSA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISYRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKSGQITYKDALKHADSPNDLRLTIKLAEEGPDQIAGTNQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=871553 RBI12_RS14290 WP_005803129.1 2972943..2974061(+) (pilU) [Acinetobacter pittii strain A774]
ATGGATTTTAATGACCTGCTCAACCTGATGGTTGAAAAAAAATCATCCGACCTCTTTATTACAGATGGCGTTGCACCATC
GATGAAGATTAACGGGCAAATTGTTCCCATTTCAAAAAATAGTCTTTCAGGCGAAATTATCGGGCAACTATTACACTCCA
TCATGAGTGAAAAACAACGCAAAGAATTTGCAGAGACTCGTGAATGTAACTTTGCGATTATGAACCGCGATAAAAGTGCG
CGCTTTCGTGTAAGTGCTTTTCAGCAACGCGACATGCCAGGCATGGTACTACGTCGAATTGAAACCAAAATTCCTTCAAT
TGACGACTTACAATTACCGCCAGTGCTTAAAGATTTATCGATGACCAAACGTGGCATCATTATTTTTGTAGGGGCAACAG
GTACAGGTAAATCGACATCATTGGCTTCAATGATTAGCTATCGTAACCATAACTCTAAAGGTCATATCATTACCATTGAA
GACCCTATCGAGTTTATTCATGAACACGCTGGCTGCATCATTACCCAACGTGAAGTCGGGATCGATACAGACTCATTTGA
AATTGCTTTAAAGAATACGTTGCGACAAGCACCCGATGTAATCTTGATTGGTGAGATTCGCTCTCGTGAAGTTATGGACT
ACGCGATTGGCTTCGCCGAAACAGGTCACCTTGTATTAGCCACAATGCATGCGAACAATGCCAACCAAGCACTTGACCGT
ATTATTCACTTCTTTGAAAGTGACCGTCATAGCCAGCTCTACATGGATTTATCACTTAACTTAAAGGCCATGATTGCACA
GCAACTTATTCCGACTCCAGATGGTAATTCGCGCCGTGCAGCGATTGAGATTCTCATTAACTCACCATTAATTTCAGACT
ATATTCGTAAAGGCGAAATTCATGAAATTAAAGATTTAATGAAACGCTCACGTGAACTCGGTATGCAAACTTTTGACCAA
GCACTGTTTGATCTTTATAAGTCAGGCCAAATTACCTACAAAGATGCATTAAAACATGCCGACTCACCAAACGATTTGCG
TTTAACAATTAAGCTTGCCGAAGAAGGTCCAGATCAAATTGCAGGCACAAATCAGCATTTAACCTTCGACCGACAGTAA

Domains

Predicted by InterProScan.

• Type II/IV secretion system protein (T2SSE) (32-270)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.397	100	0.766
pilU	Pseudomonas stutzeri DSM 10701	68.464	99.731	0.683
pilU	Vibrio cholerae strain A1552	54.324	99.462	0.54
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	40.934	97.849	0.401
pilT	Legionella pneumophila strain Lp02	39.941	90.86	0.363
pilT	Legionella pneumophila strain ERS1305867	39.941	90.86	0.363