Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   RA164_RS03490 Genome accession   NZ_CP132911
Coordinates   856248..857285 (+) Length   345 a.a.
NCBI ID   WP_329742590.1    Uniprot ID   -
Organism   Dyella aluminiiresistens strain A6     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 851248..862285
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RA164_RS03465 pdxH 851523..852110 (+) 588 WP_329742586.1 pyridoxamine 5'-phosphate oxidase -
  RA164_RS03470 - 852113..852940 (+) 828 WP_329742587.1 ABC transporter substrate-binding protein -
  RA164_RS03475 - 853258..854322 (-) 1065 WP_329742306.1 IS30 family transposase -
  RA164_RS03480 - 854568..855143 (-) 576 WP_329742588.1 YggT family protein -
  RA164_RS03485 proC 855140..855967 (-) 828 WP_329742589.1 pyrroline-5-carboxylate reductase -
  RA164_RS03490 pilT 856248..857285 (+) 1038 WP_329742590.1 type IV pilus twitching motility protein PilT Machinery gene
  RA164_RS03495 pilU 857325..858491 (+) 1167 WP_329742591.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  RA164_RS03500 - 858663..860390 (-) 1728 WP_329742592.1 hypothetical protein -
  RA164_RS03505 - 860486..861412 (-) 927 WP_329742593.1 aspartate carbamoyltransferase catalytic subunit -
  RA164_RS03510 ruvX 861409..861867 (-) 459 WP_329742594.1 Holliday junction resolvase RuvX -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38435.12 Da        Isoelectric Point: 6.8928

>NTDB_id=870588 RA164_RS03490 WP_329742590.1 856248..857285(+) (pilT) [Dyella aluminiiresistens strain A6]
MDIAELLAFSVKNKASDLHLSAGLPPMIRVDGDVRRINIPALEHKQVHALIYDIMSDKQRRDYEEFLETDFSFEIPGLAR
FRVNAFNQNRGAGAVFRTIPSEVLTLEDLGAPKIFRELIEQPQGLILVTGPTGSGKSTTLAAMVDHINKTEYGHMLTVED
PIEFVHTSQKCLVNQREVHRDTHGFNEALRSALREDPDYILVGELRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPAGEKPMVRSMLSESLRAVISQSLLKKVGGGRIAAHEIMVGIPAIRNLIREDKVAQMYSSIQTGQQFGMQTLDQNL
QDLVKRGVIARQQAQAYAKNKDIFK

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=870588 RA164_RS03490 WP_329742590.1 856248..857285(+) (pilT) [Dyella aluminiiresistens strain A6]
ATGGATATTGCCGAACTGCTTGCCTTCTCGGTGAAGAACAAAGCGTCGGATTTGCACCTTTCCGCCGGCCTGCCGCCGAT
GATCCGCGTGGACGGCGACGTGCGCCGCATCAATATCCCCGCGCTGGAGCACAAGCAGGTCCATGCGTTGATCTACGACA
TCATGTCGGACAAGCAGCGGCGCGACTACGAGGAATTCCTGGAAACCGACTTCTCGTTCGAGATCCCCGGGCTGGCTCGC
TTCCGCGTCAATGCGTTCAACCAGAATCGCGGTGCCGGCGCGGTGTTCCGTACCATTCCCTCCGAAGTGCTTACGCTGGA
GGACCTGGGTGCGCCGAAGATTTTCCGCGAACTGATCGAGCAGCCGCAGGGCCTGATCCTGGTCACCGGCCCGACCGGTT
CGGGCAAGTCCACGACGCTGGCGGCAATGGTCGACCACATCAACAAGACCGAATACGGTCACATGCTGACGGTCGAGGAC
CCGATCGAGTTTGTGCACACCTCGCAGAAGTGCCTGGTCAACCAGCGCGAGGTCCATCGCGACACGCACGGCTTCAACGA
GGCGCTGCGTTCGGCGCTGCGCGAAGATCCCGATTACATCCTGGTCGGCGAGCTGCGCGATCTTGAGACGATCCGTCTGG
CGCTTACCGCGGCGGAAACGGGCCACCTGGTGTTCGGCACCCTGCATACCAGTTCCGCGGCCAAGACCATCGACCGCATC
ATCGACGTGTTTCCTGCCGGCGAAAAGCCGATGGTCCGCTCGATGCTCTCGGAGTCGTTGCGCGCGGTGATCTCCCAGTC
GCTGCTGAAGAAAGTGGGTGGCGGGCGCATCGCGGCGCACGAGATCATGGTCGGCATTCCGGCGATCCGCAACCTGATCC
GCGAAGACAAGGTGGCGCAGATGTACTCGTCGATCCAGACCGGCCAGCAGTTCGGCATGCAGACGCTTGACCAGAACCTG
CAGGACCTGGTCAAGCGCGGCGTGATCGCGAGGCAGCAGGCGCAGGCTTATGCCAAGAACAAGGACATCTTCAAGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

79.942

99.71

0.797
  pilT Acinetobacter baumannii D1279779

79.942

99.71

0.797
  pilT Acinetobacter baumannii strain A118

79.942

99.71

0.797
  pilT Pseudomonas stutzeri DSM 10701

79.36

99.71

0.791
  pilT Acinetobacter baylyi ADP1

79.07

99.71

0.788
  pilT Pseudomonas aeruginosa PAK

78.488

99.71

0.783
  pilT Legionella pneumophila strain Lp02

75.291

99.71

0.751
  pilT Legionella pneumophila strain ERS1305867

75.291

99.71

0.751
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

71.131

97.391

0.693
  pilT Vibrio cholerae strain A1552

71.131

97.391

0.693
  pilT Neisseria meningitidis 8013

65.598

99.42

0.652
  pilT Neisseria gonorrhoeae MS11

65.306

99.42

0.649
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50

95.652

0.478
  pilU Vibrio cholerae strain A1552

40.896

97.101

0.397
  pilU Pseudomonas stutzeri DSM 10701

39.359

99.42

0.391
  pilU Acinetobacter baylyi ADP1

39.412

98.551

0.388