Detailed information    

insolico Bioinformatically predicted

Overview

Name   ruvB   Type   Machinery gene
Locus tag   AABJ50_RS10030 Genome accession   NZ_AP027523
Coordinates   2065013..2066023 (+) Length   336 a.a.
NCBI ID   WP_000568519.1    Uniprot ID   Q32HA1
Organism   Escherichia coli strain LR-33     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2060013..2071023
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AABJ50_RS10000 (VEE12_19300) nudB 2061737..2062189 (+) 453 WP_001300367.1 dihydroneopterin triphosphate diphosphatase -
  AABJ50_RS10005 (VEE12_19310) yebC 2062218..2062958 (+) 741 WP_000907234.1 YebC/PmpR family DNA-binding transcriptional regulator -
  AABJ50_RS10010 (VEE12_19320) ruvC 2062993..2063514 (+) 522 WP_001295503.1 crossover junction endodeoxyribonuclease RuvC -
  AABJ50_RS10015 (VEE12_19330) yebB 2063516..2064118 (-) 603 WP_001024904.1 YebB family permuted papain-like enzyme -
  AABJ50_RS10020 yobI 2064189..2064254 (+) 66 WP_072094247.1 stress response small protein YobI -
  AABJ50_RS10025 (VEE12_19340) ruvA 2064393..2065004 (+) 612 WP_000580327.1 Holliday junction branch migration protein RuvA -
  AABJ50_RS10030 (VEE12_19350) ruvB 2065013..2066023 (+) 1011 WP_000568519.1 Holliday junction branch migration DNA helicase RuvB Machinery gene
  AABJ50_RS10035 (VEE12_19360) znuB 2066170..2066955 (-) 786 WP_000571479.1 zinc ABC transporter permease subunit ZnuB -
  AABJ50_RS10040 (VEE12_19370) znuC 2066952..2067707 (-) 756 WP_000202990.1 zinc ABC transporter ATP-binding protein ZnuC -
  AABJ50_RS10045 (VEE12_19380) znuA 2067786..2068718 (+) 933 WP_001300644.1 zinc ABC transporter substrate-binding protein ZnuA -
  AABJ50_RS10050 (VEE12_19390) mepM 2068734..2070056 (+) 1323 WP_001184045.1 murein DD-endopeptidase MepM -

Sequence

Protein


Download         Length: 336 a.a.        Molecular weight: 37173.77 Da        Isoelectric Point: 4.7818

>NTDB_id=86954 AABJ50_RS10030 WP_000568519.1 2065013..2066023(+) (ruvB) [Escherichia coli strain LR-33]
MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIFIKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMG
VNLRTTSGPVLEKAGDLAAMLTNLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGALEVARRARGTPRIANRLLRRVRDFAEVKHDG
TISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKFFGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMA
TTRAWNHFGITPPEMP

Nucleotide


Download         Length: 1011 bp        

>NTDB_id=86954 AABJ50_RS10030 WP_000568519.1 2065013..2066023(+) (ruvB) [Escherichia coli strain LR-33]
ATGATTGAAGCAGACCGTCTGATTTCTGCCGGTACCACTTTGCCGGAAGATGTAGCAGATCGCGCCATTCGCCCTAAATT
ACTGGAAGAGTATGTTGGTCAGCCGCAGGTTCGTTCACAGATGGAGATTTTCATCAAAGCAGCGAAACTGCGCGGCGATG
CCCTCGATCATTTGTTGATTTTTGGTCCTCCGGGGTTGGGTAAAACTACGCTTGCCAACATTGTCGCCAATGAAATGGGC
GTTAATTTACGCACGACTTCTGGTCCGGTGCTGGAAAAGGCGGGCGATTTGGCTGCGATGCTCACTAACCTTGAACCGCA
TGACGTGCTGTTTATTGATGAGATCCACCGTTTATCGCCAGTTGTTGAAGAAGTGCTGTACCCGGCAATGGAAGACTACC
AACTGGATATCATGATTGGTGAAGGTCCGGCGGCACGCTCCATTAAAATTGATTTGCCGCCGTTTACCCTGATTGGTGCA
ACCACGCGCGCAGGTTCGCTGACATCACCGTTGCGCGACCGTTTTGGTATTGTGCAACGTCTGGAGTTTTATCAGGTGCC
GGATCTGCAATATATCGTCAGTCGCAGCGCACGCTTTATGGGGCTTGAGATGAGTGATGACGGCGCGCTGGAAGTTGCTC
GTCGCGCTCGCGGTACGCCGCGCATTGCCAACCGTCTGCTGCGTCGAGTGCGTGATTTCGCCGAAGTGAAGCACGATGGC
ACCATCTCGGCAGATATCGCTGCTCAGGCGTTGGATATGTTGAATGTCGATGCTGAAGGTTTCGATTATATGGACCGCAA
ATTGTTGCTGGCGGTAATCGATAAGTTCTTTGGTGGGCCGGTAGGTCTGGATAACCTTGCGGCAGCCATTGGCGAAGAAC
GTGAAACCATTGAGGATGTGCTGGAACCTTATTTGATTCAGCAAGGCTTTTTGCAGCGTACACCGCGTGGGCGTATGGCG
ACGACGCGGGCGTGGAATCACTTTGGCATAACGCCGCCAGAAATGCCGTAA

Domains

Predicted by InterProScan.

Holliday junction DNA helicase RuvB P-loop domain (RuvB_N)

(23-181)

RuvB AAA lid domain (AAA_lid_4)

(184-257)

RuvB C-terminal winged helix domain (WHD_RuvB)

(259-328)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q32HA1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ruvB Bacillus subtilis subsp. subtilis str. 168

60.486

97.917

0.592
  ruvB Streptococcus pneumoniae TIGR4

58.934

94.94

0.56
  ruvB Streptococcus pneumoniae R6

58.934

94.94

0.56
  ruvB Streptococcus pneumoniae D39

58.934

94.94

0.56
  ruvB Synechocystis sp. PCC 6803

53.251

96.131

0.512
  ruvB Helicobacter pylori 26695

52.038

94.94

0.494

Multiple sequence alignment