Detailed information NTG0086874

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: K6K01_RS13680
• Genome accession: NZ_AP024622
• Coordinates: 2619680..2620297 (-)
• Length: 205 a.a.
• NCBI ID: WP_004398514.1
• Uniprot ID: A0AAE2SLR2
• Organism: Bacillus subtilis strain BEST3096
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2614680..2625297

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
K6K01_RS13650 (BsBEST3096_26150)	rpsT	2614982..2615248 (+)	267	WP_003229989.1	30S ribosomal protein S20	-
K6K01_RS13655 (BsBEST3096_26160)	holA	2615263..2616306 (-)	1044	WP_003229987.1	DNA polymerase III subunit delta	-
K6K01_RS13660	-	2616346..2616495 (-)	150	WP_003229985.1	hypothetical protein	-
K6K01_RS13665 (BsBEST3096_26170)	yqzM	2616536..2616670 (+)	135	WP_003229983.1	YqzM family protein	-
K6K01_RS13670	comEC	2616710..2619040 (-)	2331	WP_009967776.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
K6K01_RS13675 (BsBEST3096_26200)	comEB	2619044..2619613 (-)	570	WP_003229978.1	ComE operon protein 2	-
K6K01_RS13680 (BsBEST3096_26210)	comEA	2619680..2620297 (-)	618	WP_004398514.1	competence protein ComEA	Machinery gene
K6K01_RS13685 (BsBEST3096_26220)	comER	2620381..2621202 (+)	822	WP_004398597.1	late competence protein ComER	-
K6K01_RS13690 (BsBEST3096_26230)	yqeM	2621268..2622011 (-)	744	WP_003229973.1	class I SAM-dependent methyltransferase	-
K6K01_RS13695 (BsBEST3096_26240)	rsfS	2622008..2622364 (-)	357	WP_003229971.1	ribosome silencing factor	-
K6K01_RS13700 (BsBEST3096_26250)	yqeK	2622382..2622942 (-)	561	WP_004399059.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
K6K01_RS13705 (BsBEST3096_26260)	nadD	2622932..2623501 (-)	570	WP_004398676.1	nicotinate-nucleotide adenylyltransferase	-
K6K01_RS13710 (BsBEST3096_26270)	yhbY	2623513..2623803 (-)	291	WP_003226133.1	ribosome assembly RNA-binding protein YhbY	-
K6K01_RS13715 (BsBEST3096_26280)	aroE	2623797..2624639 (-)	843	WP_003229967.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 205 a.a.
• Molecular weight: 21769.45 Da
• Isoelectric Point: 4.7220

>NTDB_id=86874 K6K01_RS13680 WP_004398514.1 2619680..2620297(-) (comEA) [Bacillus subtilis strain BEST3096]
MNWLNQHKKAIILAASAAVFTAIMIFLATGKNKEPVKQAVPTETENTVVKQEANNDESNETIVIDIKGAVQHPGVYEMRT
GDRVSQAIEKAGGTSEQADEAQVNLAEILQDGTVVYIPKKGEETAVQQGGGGSVQSDGGKGALVNINTATLEELQGISGV
GPSKAEAIIAYREENGRFQTIEDITKVSGIGEKSFEKIKSSITVK

Nucleotide

• Length: 618 bp

>NTDB_id=86874 K6K01_RS13680 WP_004398514.1 2619680..2620297(-) (comEA) [Bacillus subtilis strain BEST3096]
ATGAATTGGTTGAATCAGCATAAGAAAGCAATTATTTTAGCGGCTTCTGCGGCTGTTTTCACAGCGATTATGATCTTTCT
GGCCACAGGGAAAAATAAAGAGCCGGTGAAGCAAGCTGTACCAACAGAGACAGAAAATACAGTGGTAAAGCAGGAAGCAA
ACAACGACGAGTCAAACGAAACAATTGTGATAGACATCAAAGGTGCTGTTCAGCATCCTGGCGTTTATGAAATGCGAACA
GGGGACAGAGTATCTCAGGCAATTGAGAAAGCGGGCGGGACCAGTGAACAAGCAGACGAAGCGCAAGTAAATTTGGCGGA
GATTCTGCAGGACGGGACAGTGGTGTACATCCCGAAAAAGGGAGAGGAAACAGCAGTGCAGCAAGGTGGCGGAGGGTCTG
TCCAAAGCGATGGAGGGAAGGGAGCGCTGGTGAATATCAATACAGCAACCTTAGAGGAGTTACAAGGCATCTCAGGGGTG
GGGCCATCCAAAGCTGAAGCTATTATTGCATACCGGGAGGAAAACGGTCGTTTCCAAACAATTGAAGATATCACTAAGGT
TTCAGGAATAGGTGAAAAGTCATTTGAGAAAATAAAGTCTTCCATTACAGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (142-203)

• SLBB domain (SLBB) (63-118)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	100	100	1
comEA	Staphylococcus aureus MW2	37.273	100	0.4
comEA	Staphylococcus aureus N315	36.364	100	0.39