Detailed information    

insolico Bioinformatically predicted

Overview

Name   dprA   Type   Machinery gene
Locus tag   RAM32_RS10010 Genome accession   NZ_CP132361
Coordinates   2009528..2010400 (+) Length   290 a.a.
NCBI ID   WP_000593194.1    Uniprot ID   A0A9P3DKN4
Organism   Staphylococcus aureus strain UNC_SA56     
Function   ssDNA binding; loading RecA onto ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 2004528..2015400
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RAM32_RS09990 (RAM32_09985) sucC 2004642..2005808 (+) 1167 WP_001020801.1 ADP-forming succinate--CoA ligase subunit beta -
  RAM32_RS09995 (RAM32_09990) sucD 2005830..2006738 (+) 909 WP_000110253.1 succinate--CoA ligase subunit alpha -
  RAM32_RS10000 (RAM32_09995) - 2006965..2008083 (+) 1119 Protein_1960 LysM peptidoglycan-binding domain-containing protein -
  RAM32_RS10005 (RAM32_10000) fmhC 2008111..2009355 (+) 1245 WP_000672869.1 FemA/FemB family glycyltransferase FmhC -
  RAM32_RS10010 (RAM32_10005) dprA 2009528..2010400 (+) 873 WP_000593194.1 DNA-processing protein DprA Machinery gene
  RAM32_RS10015 (RAM32_10010) topA 2010574..2012649 (+) 2076 WP_306181351.1 type I DNA topoisomerase -
  RAM32_RS10020 (RAM32_10015) trmFO 2012805..2014112 (+) 1308 WP_000195254.1 methylenetetrahydrofolate--tRNA-(uracil(54)- C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO -

Sequence

Protein


Download         Length: 290 a.a.        Molecular weight: 33541.01 Da        Isoelectric Point: 9.5093

>NTDB_id=868448 RAM32_RS10010 WP_000593194.1 2009528..2010400(+) (dprA) [Staphylococcus aureus strain UNC_SA56]
MIKLFLLKLYWAHFSTKQIHQFLMAYPNVIKEEGRKKDSYLCEWVNREENVLLLRKYYAFIKLDHNDIIKELQKLKVSYI
TYMDSEYPVLLKEIYQFPLLLFYKGNIKLINNMHHLAVVGARDSTSYTQQSLEFLLSNDKSKYLTIVSGLAQGADAMAHQ
IALKYNLPTIAVLAFGHQTHYPKSTLALRNKIEEKGLVISEYPPHTPIAKYRFPERNRIISGLSKGVLITEAKEQSGSHI
TIDFALEQNRNVYVLPGSMFNPMTKGNLLRIQEGAKVVLNANDIFEDYYI

Nucleotide


Download         Length: 873 bp        

>NTDB_id=868448 RAM32_RS10010 WP_000593194.1 2009528..2010400(+) (dprA) [Staphylococcus aureus strain UNC_SA56]
TTGATTAAACTATTTTTGCTTAAGTTATACTGGGCACACTTTTCGACTAAACAAATTCATCAATTTTTAATGGCATATCC
TAATGTAATTAAAGAGGAGGGAAGAAAAAAAGATAGTTATTTATGTGAATGGGTGAATAGGGAAGAAAATGTTCTTTTAT
TACGTAAATACTATGCTTTTATAAAACTTGATCATAACGATATTATTAAAGAACTGCAGAAATTAAAAGTAAGTTACATT
ACATATATGGATTCTGAATACCCAGTGCTATTAAAAGAAATATATCAATTTCCATTACTTCTTTTCTATAAAGGGAACAT
CAAATTAATAAATAATATGCATCATTTAGCAGTAGTAGGTGCAAGAGATTCTACAAGTTATACCCAACAGTCTTTAGAAT
TTTTATTATCAAATGATAAAAGCAAATATTTAACAATTGTTTCCGGCCTTGCTCAAGGAGCTGATGCAATGGCACATCAA
ATAGCTTTAAAATACAATCTCCCTACAATTGCAGTTTTAGCCTTTGGCCATCAAACACATTATCCCAAAAGTACATTAGC
ATTAAGAAATAAAATAGAAGAAAAAGGTTTAGTTATATCCGAATATCCACCACATACACCAATTGCTAAATATAGATTTC
CTGAGCGCAATAGAATTATCAGCGGTTTGTCAAAAGGGGTTTTAATTACTGAGGCTAAGGAACAAAGTGGCAGTCACATC
ACGATAGATTTTGCATTAGAGCAAAATAGAAATGTTTATGTTTTACCTGGATCTATGTTTAATCCTATGACAAAAGGTAA
TTTATTACGTATCCAAGAAGGAGCTAAGGTAGTATTAAACGCTAATGATATATTTGAAGACTACTATATTTAA

Domains

Predicted by InterproScan.

DNA recombination-mediator protein A (DNA_processg_A)

(72-279)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  dprA Staphylococcus aureus N315

100

100

1
  dprA Staphylococcus aureus MW2

99.655

100

0.997
  dprA/cilB/dalA Streptococcus pneumoniae Rx1

37.809

97.586

0.369
  dprA/cilB/dalA Streptococcus pneumoniae TIGR4

37.809

97.586

0.369
  dprA/cilB/dalA Streptococcus pneumoniae D39

37.809

97.586

0.369
  dprA/cilB/dalA Streptococcus pneumoniae R6

37.809

97.586

0.369
  dprA/cilB/dalA Streptococcus mitis SK321

37.456

97.586

0.366