Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   Q8X41_RS06675 Genome accession   NZ_CP132291
Coordinates   1337084..1338127 (-) Length   347 a.a.
NCBI ID   WP_397539474.1    Uniprot ID   -
Organism   Rummeliibacillus pycnus strain X33     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1332084..1343127
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  Q8X41_RS06650 - 1332789..1333601 (-) 813 WP_397539470.1 hypothetical protein -
  Q8X41_RS06655 - 1333598..1334185 (-) 588 WP_397539471.1 PilN domain-containing protein -
  Q8X41_RS06660 pilM 1334182..1335162 (-) 981 WP_397539472.1 type IV pilus biogenesis protein PilM -
  Q8X41_RS06665 - 1335296..1335850 (-) 555 WP_397540211.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  Q8X41_RS06670 - 1335882..1337087 (-) 1206 WP_397539473.1 type II secretion system F family protein -
  Q8X41_RS06675 pilT 1337084..1338127 (-) 1044 WP_397539474.1 type IV pilus twitching motility protein PilT Machinery gene
  Q8X41_RS06680 - 1338139..1339803 (-) 1665 WP_397539475.1 GspE/PulE family protein -
  Q8X41_RS06685 - 1339823..1341199 (-) 1377 WP_397539476.1 VanW family protein -
  Q8X41_RS06690 - 1341635..1342090 (+) 456 WP_397539477.1 hypothetical protein -
  Q8X41_RS06695 - 1342090..1342539 (+) 450 WP_397539478.1 type II secretion system protein J -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38749.46 Da        Isoelectric Point: 7.1420

>NTDB_id=868006 Q8X41_RS06675 WP_397539474.1 1337084..1338127(-) (pilT) [Rummeliibacillus pycnus strain X33]
MTKSIVELLTYADEKDASDLHATVGVPPYYRINGQLLQHGEEILTPKDLEQMLREVLPPHRMKEFETNGEVDFNYSLPGR
CRFRMNAYHQRNSCTLAARKITAEIPTIEQLGMPEVLKDLCNKPQGLILVTGPTGSGKSTTLAAMINYINTNQAKHIITL
EDPIEYLHKHQQSIVNQREVGSDTKSFANGLRAALRQDPDIILVGEMRDLETISTAITAAETGHLVMATLHTSSAPTTID
RIIDVFPSHQQGQIRIQLANVLQGIISQRLFKRVDKPGRIAATEILVHTSSVANLIRNDKVHQLTNIMQTNRALGMHTID
SSIQQLLAKRLIDFETAKVYMNAGEFV

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=868006 Q8X41_RS06675 WP_397539474.1 1337084..1338127(-) (pilT) [Rummeliibacillus pycnus strain X33]
ATGACAAAGTCAATCGTAGAATTACTAACTTATGCGGATGAAAAGGATGCTTCCGATTTACATGCAACGGTAGGGGTACC
TCCATACTATCGAATTAATGGGCAATTGCTTCAACATGGAGAAGAAATTTTGACCCCTAAAGATTTAGAGCAGATGCTTC
GAGAAGTGTTGCCACCACATCGTATGAAAGAATTTGAAACAAATGGGGAAGTAGACTTTAACTACTCATTGCCTGGAAGA
TGTCGTTTCCGCATGAACGCTTACCATCAACGAAATAGTTGCACACTAGCAGCTCGTAAAATCACTGCAGAAATACCAAC
GATTGAGCAATTAGGAATGCCAGAAGTGTTGAAGGATTTATGCAATAAACCACAAGGGTTAATTTTAGTAACAGGTCCCA
CAGGTTCTGGTAAATCAACAACGCTGGCAGCCATGATTAACTATATCAATACAAACCAAGCAAAACATATTATTACGCTT
GAAGATCCAATCGAGTATTTGCATAAGCATCAACAATCGATTGTCAACCAACGTGAAGTCGGATCAGATACAAAAAGCTT
TGCCAACGGTTTACGAGCAGCACTTCGACAAGACCCGGATATTATTCTAGTAGGGGAAATGCGAGATTTAGAGACGATTT
CAACAGCCATAACTGCGGCTGAAACAGGTCACTTAGTCATGGCTACATTGCATACAAGTAGCGCTCCTACAACGATTGAC
CGTATTATCGATGTGTTTCCATCACATCAACAAGGGCAAATTCGTATTCAGTTGGCAAATGTATTGCAAGGTATCATTTC
GCAACGTCTATTTAAACGAGTAGACAAGCCTGGTCGAATTGCCGCTACTGAAATTTTGGTACACACGTCTTCTGTTGCAA
ACTTGATCCGTAATGACAAAGTGCATCAGCTGACGAATATTATGCAAACAAACCGCGCGCTTGGCATGCATACGATTGAT
AGCTCGATTCAACAGTTACTTGCAAAGAGACTAATCGATTTCGAAACGGCTAAAGTTTATATGAATGCAGGTGAATTTGT
ATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(107-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

51.368

94.813

0.487
  pilT Vibrio cholerae strain A1552

51.368

94.813

0.487
  pilT Pseudomonas stutzeri DSM 10701

49.102

96.254

0.473
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.547

95.389

0.473
  pilT Pseudomonas aeruginosa PAK

48.802

96.254

0.47
  pilT Legionella pneumophila strain ERS1305867

47.447

95.965

0.455
  pilT Legionella pneumophila strain Lp02

47.447

95.965

0.455
  pilT Acinetobacter baumannii D1279779

47.006

96.254

0.452
  pilT Acinetobacter baumannii strain A118

47.006

96.254

0.452
  pilT Acinetobacter baylyi ADP1

46.041

98.271

0.452
  pilT Acinetobacter nosocomialis M2

46.707

96.254

0.45
  pilT Neisseria meningitidis 8013

46.407

96.254

0.447
  pilT Neisseria gonorrhoeae MS11

46.108

96.254

0.444
  pilU Pseudomonas stutzeri DSM 10701

43.323

97.118

0.421
  pilU Vibrio cholerae strain A1552

42.765

89.625

0.383
  pilU Acinetobacter baylyi ADP1

37.651

95.677

0.36