Detailed information NTG0865087

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: Q7588_RS02130
• Genome accession: NZ_CP131787
• Coordinates: 458724..459872 (+)
• Length: 382 a.a.
• NCBI ID: WP_003100959.1
• Uniprot ID: G3XCX3
• Organism: Pseudomonas aeruginosa strain 2014S07-062
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 453724..464872

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
Q7588_RS02105 (Q7588_02105)	-	454875..455171 (-)	297	WP_031628111.1	DUF167 domain-containing protein	-
Q7588_RS02110 (Q7588_02110)	-	455168..455761 (-)	594	WP_003084544.1	YggT family protein	-
Q7588_RS02115 (Q7588_02115)	proC	455772..456593 (-)	822	WP_003084546.1	pyrroline-5-carboxylate reductase	-
Q7588_RS02120 (Q7588_02120)	-	456605..457297 (-)	693	WP_003084549.1	YggS family pyridoxal phosphate-dependent enzyme	-
Q7588_RS02125 (Q7588_02125)	pilT	457512..458546 (+)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
Q7588_RS02130 (Q7588_02130)	pilU	458724..459872 (+)	1149	WP_003100959.1	type IV pilus ATPase PilU	Machinery gene
Q7588_RS02135 (Q7588_02135)	czcD	459880..460779 (-)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
Q7588_RS02140 (Q7588_02140)	-	460934..461338 (+)	405	WP_003084560.1	NINE protein	-
Q7588_RS02145 (Q7588_02145)	-	461581..462954 (+)	1374	WP_015502220.1	pyridoxal-phosphate dependent enzyme	-
Q7588_RS02150 (Q7588_02150)	-	462951..464135 (+)	1185	WP_003117897.1	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42532.81 Da
• Isoelectric Point: 6.7792

>NTDB_id=865087 Q7588_RS02130 WP_003100959.1 458724..459872(+) (pilU) [Pseudomonas aeruginosa strain 2014S07-062]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSSLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=865087 Q7588_RS02130 WP_003100959.1 458724..459872(+) (pilU) [Pseudomonas aeruginosa strain 2014S07-062]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAGCCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: G3XCX3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.482	100	0.885
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382