Detailed information    

insolico Bioinformatically predicted

Overview

Name   stkP   Type   Regulator
Locus tag   WFE12_RS08650 Genome accession   NZ_CP147877
Coordinates   1652557..1654503 (+) Length   648 a.a.
NCBI ID   WP_003232062.1    Uniprot ID   O34507
Organism   Bacillus subtilis subsp. subtilis str. 168     
Function   require for competence development; phosphorylate ComE (predicted from homology)   
Competence regulation

Genomic Context

Location: 1647557..1659503
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WFE12_RS08625 def 1647927..1648409 (+) 483 WP_003232077.1 peptide deformylase -
  WFE12_RS08630 fmt 1648414..1649367 (+) 954 WP_003232070.1 methionyl-tRNA formyltransferase -
  WFE12_RS08635 rsmB 1649354..1650697 (+) 1344 WP_003232068.1 16S rRNA (cytosine(967)-C(5))-methyltransferase RsmB -
  WFE12_RS08640 rlmN 1650701..1651792 (+) 1092 WP_003232066.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  WFE12_RS08645 prpC 1651799..1652563 (+) 765 WP_003232064.1 protein-serine/threonine phosphatase PrpC -
  WFE12_RS08650 stkP 1652557..1654503 (+) 1947 WP_003232062.1 serine/threonine protein kinase PrkC Regulator
  WFE12_RS08655 rsgA 1654518..1655414 (+) 897 WP_003232060.1 ribosome small subunit-dependent GTPase A -
  WFE12_RS08660 rpe 1655419..1656072 (+) 654 WP_003232058.1 ribulose-phosphate 3-epimerase -
  WFE12_RS08665 thiN 1656145..1656789 (+) 645 WP_003245470.1 thiamine diphosphokinase -
  WFE12_RS08670 spoVM 1656861..1656941 (+) 81 WP_003221545.1 stage V sporulation protein SpoVM -
  WFE12_RS08675 rpmB 1657014..1657202 (-) 189 WP_003221548.1 50S ribosomal protein L28 -
  WFE12_RS08680 yloU 1657479..1657841 (+) 363 WP_003232054.1 Asp23/Gls24 family envelope stress response protein -

Sequence

Protein


Download         Length: 648 a.a.        Molecular weight: 71866.49 Da        Isoelectric Point: 4.7568

>NTDB_id=863284 WFE12_RS08650 WP_003232062.1 1652557..1654503(+) (stkP) [Bacillus subtilis subsp. subtilis str. 168]
MLIGKRISGRYQILRVIGGGGMANVYLAEDIILDREVAIKILRFDYANDNEFIRRFRREAQSASSLDHPNIVSIYDLGEE
DDIYYIVMEYVEGMTLKEYITANGPLHPKEALNIMEQIVSAIAHAHQNQIVHRDIKPHNILIDHMGNIKVTDFGIATALS
STTITHTNSVLGSVHYLSPEQARGGLATKKSDIYALGIVLFELLTGRIPFDGESAVSIALKHLQAETPSAKRWNPSVPQS
VENIILKATAKDPFHRYETAEDMEADIKTAFDADRLNEKRFTIQEDEEMTKAIPIIKDEELAKAAGEKEAEVTTAQENKT
KKNGKRKKWPWVLLTICLVFITAGILAVTVFPSLFMPKDVKIPDVSGMEYEKAAGLLEKEGLQVDSEVLEISDEKIEEGL
MVKTDPKADTTVKEGATVTLYKSTGKAKTEIGDVTGQTVDQAKKALKDQGFNHVTVNEVNDEKNAGTVIDQNPSAGTELV
PSEDQVKLTVSIGPEDITLRDLKTYSKEAASGYLEDNGLKLVEKEAYSDDVPEGQVVKQKPAAGTAVKPGNEVEVTFSLG
PEKKPAKTVKEKVKIPYEPENEGDELQVQIAVDDADHSISDTYEEFKIKEPTERTIELKIEPGQKGYYQVMVNNKVVSYK
TIEYPKDE

Nucleotide


Download         Length: 1947 bp        

>NTDB_id=863284 WFE12_RS08650 WP_003232062.1 1652557..1654503(+) (stkP) [Bacillus subtilis subsp. subtilis str. 168]
GTGCTAATCGGCAAGCGGATCAGCGGGCGTTACCAAATTCTCCGCGTCATAGGCGGCGGGGGAATGGCCAACGTGTATTT
AGCTGAGGATATCATTCTAGACCGTGAAGTCGCAATTAAAATCCTGCGGTTTGACTATGCAAATGACAATGAATTTATCA
GACGTTTCCGCAGAGAAGCCCAATCCGCATCAAGCCTCGATCACCCGAATATTGTCAGCATTTATGATTTGGGCGAGGAA
GATGATATTTATTATATTGTCATGGAATACGTTGAAGGCATGACGCTTAAAGAATACATAACAGCAAATGGGCCGCTTCA
CCCTAAAGAAGCGCTGAACATCATGGAGCAAATTGTCTCAGCCATCGCTCATGCCCATCAAAACCAGATTGTTCACAGAG
ACATCAAGCCGCATAACATTTTGATTGATCATATGGGAAATATCAAAGTAACGGATTTTGGAATTGCGACGGCACTAAGT
TCGACCACAATCACCCATACCAATTCAGTTCTGGGCTCGGTCCATTACTTATCACCTGAACAGGCCCGGGGCGGCTTAGC
CACAAAAAAATCGGATATTTATGCGCTTGGAATCGTTCTATTTGAGCTTTTAACCGGCCGTATTCCGTTTGATGGAGAGT
CAGCAGTCAGCATCGCCTTGAAGCATCTTCAAGCGGAAACTCCTTCGGCAAAAAGGTGGAATCCATCGGTCCCCCAAAGC
GTTGAAAACATCATACTCAAGGCAACTGCCAAAGATCCTTTTCATCGCTACGAAACGGCTGAAGACATGGAAGCAGACAT
AAAAACAGCTTTTGATGCCGACAGACTCAATGAAAAGAGATTTACGATTCAAGAAGATGAAGAAATGACAAAAGCGATAC
CTATCATTAAAGATGAAGAACTTGCTAAAGCTGCTGGCGAAAAAGAAGCTGAAGTGACAACCGCACAAGAAAACAAAACA
AAGAAGAACGGCAAAAGAAAAAAGTGGCCGTGGGTTTTGCTCACGATATGCCTCGTTTTTATCACAGCTGGAATTCTTGC
TGTCACTGTTTTTCCGTCGCTTTTCATGCCTAAGGATGTCAAAATACCTGATGTCTCCGGAATGGAATACGAAAAAGCCG
CAGGGCTCTTGGAAAAAGAAGGTTTACAGGTTGATTCCGAGGTGTTGGAAATCTCAGATGAAAAAATTGAAGAGGGCCTG
ATGGTAAAAACGGATCCTAAAGCGGATACCACAGTCAAAGAAGGCGCCACGGTCACCCTTTATAAGAGCACCGGAAAAGC
AAAAACGGAGATCGGTGATGTGACAGGCCAAACGGTCGACCAAGCAAAAAAAGCGTTGAAGGACCAAGGGTTTAATCATG
TAACAGTAAATGAAGTGAATGACGAGAAAAATGCGGGCACTGTCATTGACCAAAATCCTTCAGCAGGGACTGAGCTGGTC
CCGAGTGAAGATCAAGTCAAACTTACAGTCAGTATCGGACCCGAAGACATTACGCTTAGAGACTTGAAAACCTACAGTAA
AGAAGCAGCGTCTGGATATCTGGAAGACAACGGATTGAAGCTTGTAGAAAAAGAAGCATACTCAGATGATGTTCCAGAAG
GACAGGTTGTCAAACAAAAACCAGCAGCAGGTACGGCAGTAAAGCCGGGAAACGAAGTTGAAGTGACATTCTCTCTCGGA
CCAGAGAAAAAACCTGCGAAAACAGTGAAAGAAAAGGTCAAGATCCCCTACGAACCAGAAAATGAAGGGGACGAGCTTCA
AGTGCAAATCGCGGTTGACGATGCGGATCACAGCATCTCTGACACTTACGAAGAATTTAAGATAAAAGAGCCGACTGAAC
GAACGATCGAACTAAAGATTGAACCAGGCCAAAAAGGGTACTATCAAGTAATGGTAAACAATAAAGTTGTCAGCTACAAA
ACCATTGAGTATCCGAAAGATGAATAA

Domains

Predicted by InterProScan.

Protein kinase domain (Pkinase)

(11-261)

PASTA domain (PASTA)

(360-423)

PASTA domain (PASTA)

(430-491)

PASTA domain (PASTA)

(498-558)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB O34507

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  stkP Streptococcus pneumoniae TIGR4

40.555

89.043

0.361