Detailed information    

insolico Bioinformatically predicted

Overview

Name   dprA   Type   Machinery gene
Locus tag   LLUC029_RS05970 Genome accession   NZ_CP129163
Coordinates   1170232..1171080 (-) Length   282 a.a.
NCBI ID   WP_032950088.1    Uniprot ID   Q25AX2
Organism   Lactococcus cremoris strain UC029     
Function   ssDNA binding; loading RecA onto ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 1165232..1176080
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LLUC029_RS05955 (LLUC029_05970) xerS 1165302..1166372 (+) 1071 WP_014572599.1 tyrosine recombinase XerS -
  LLUC029_RS05960 (LLUC029_05975) trmFO 1166422..1167768 (-) 1347 WP_032950090.1 methylenetetrahydrofolate--tRNA-(uracil(54)- C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO -
  LLUC029_RS05965 (LLUC029_05980) topA 1167840..1169966 (-) 2127 WP_015082465.1 type I DNA topoisomerase -
  LLUC029_RS05970 (LLUC029_05985) dprA 1170232..1171080 (-) 849 WP_032950088.1 DNA-processing protein DprA Machinery gene
  LLUC029_RS05975 (LLUC029_05990) - 1171229..1171609 (-) 381 WP_014572603.1 RidA family protein -
  LLUC029_RS05980 (LLUC029_05995) budA 1171649..1172359 (-) 711 WP_011676302.1 acetolactate decarboxylase -
  LLUC029_RS05985 (LLUC029_06000) ilvA 1172494..1173744 (-) 1251 WP_032950084.1 threonine ammonia-lyase IlvA -
  LLUC029_RS05990 (LLUC029_06005) ilvC 1173803..1174825 (-) 1023 WP_021211415.1 ketol-acid reductoisomerase -
  LLUC029_RS05995 (LLUC029_06010) ilvN 1174871..1175347 (-) 477 WP_032950082.1 acetolactate synthase small subunit -

Sequence

Protein


Download         Length: 282 a.a.        Molecular weight: 31955.09 Da        Isoelectric Point: 9.8385

>NTDB_id=851343 LLUC029_RS05970 WP_032950088.1 1170232..1171080(-) (dprA) [Lactococcus cremoris strain UC029]
MITNFDLFRWKKAGMTNLGVNKLLKFFRKYDIKISLRQMGQVAQVKSIPNFIEQYKNQDVKKLRIDYKKFSSFSILDDLY
PERLREIYNPPVLIFYQGNIDLLKNPKLAFVGSRLAGQSGIKAVQKIITELNQSFTIVSGLAKGIDTASHLSAIKTKTPT
IAVIGTGLDIFYPLENRKIQEYLAKNQLVLSEYSLGEKPLKYHFPERNRIIAGLSRGVVVVEAKLRSGSLITCERALEEG
RDIFAIPGNIADGTSDDCNHLIQQGAKLVYQAQDILEEYLYN

Nucleotide


Download         Length: 849 bp        

>NTDB_id=851343 LLUC029_RS05970 WP_032950088.1 1170232..1171080(-) (dprA) [Lactococcus cremoris strain UC029]
ATGATTACAAATTTTGATTTATTTCGTTGGAAAAAAGCAGGAATGACCAATCTTGGTGTAAATAAGCTCTTAAAATTTTT
CCGCAAATATGATATAAAAATAAGCTTACGACAAATGGGACAAGTGGCTCAAGTCAAATCAATCCCTAACTTTATCGAAC
AATATAAAAATCAGGACGTAAAGAAACTCAGGATAGACTATAAAAAATTTTCCTCCTTTTCCATTCTTGATGACCTTTAT
CCCGAGCGTCTAAGAGAAATCTACAACCCACCCGTTTTAATTTTTTATCAGGGAAATATTGATTTATTAAAAAATCCTAA
ACTTGCCTTTGTTGGAAGCCGCCTAGCAGGTCAATCAGGGATAAAAGCTGTCCAAAAAATTATCACAGAATTGAATCAAT
CCTTTACAATAGTAAGCGGGCTTGCTAAAGGGATTGACACAGCCAGCCATCTGTCAGCAATTAAAACTAAAACACCAACA
ATTGCTGTTATTGGTACAGGATTGGATATTTTCTATCCACTAGAAAATCGTAAAATACAGGAATATCTAGCAAAAAATCA
ATTAGTTTTAAGTGAATATTCATTAGGCGAAAAACCACTTAAATACCACTTTCCAGAGCGCAATCGAATTATTGCTGGTC
TTTCACGGGGAGTTGTTGTCGTGGAAGCAAAATTAAGAAGTGGCAGTTTAATCACCTGTGAAAGGGCACTGGAAGAAGGA
AGAGATATCTTTGCCATCCCTGGAAATATTGCAGATGGAACATCTGATGACTGTAATCATCTTATTCAACAAGGAGCAAA
ACTTGTCTATCAAGCTCAAGATATTTTAGAAGAATATTTGTACAATTAA

Domains

Predicted by InterproScan.

DNA recombination-mediator protein A (DNA_processg_A)

(69-270)

DNA processing protein A sterile alpha motif domain (SAM_DprA)

(2-63)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q25AX2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  dprA Lactococcus lactis subsp. cremoris KW2

97.872

100

0.979
  dprA Streptococcus mutans UA159

58.993

98.582

0.582
  dprA/cilB/dalA Streptococcus mitis SK321

55.714

99.291

0.553
  dprA/cilB/dalA Streptococcus pneumoniae R6

55.357

99.291

0.55
  dprA/cilB/dalA Streptococcus pneumoniae TIGR4

55.357

99.291

0.55
  dprA/cilB/dalA Streptococcus mitis NCTC 12261

55.357

99.291

0.55
  dprA/cilB/dalA Streptococcus pneumoniae Rx1

55.357

99.291

0.55
  dprA/cilB/dalA Streptococcus pneumoniae D39

55.357

99.291

0.55
  dprA Vibrio campbellii strain DS40M4

35.314

100

0.379
  dprA Bacillus subtilis subsp. subtilis str. 168

38.909

97.518

0.379
  dprA Legionella pneumophila strain ERS1305867

38.768

97.872

0.379
  dprA Haemophilus influenzae Rd KW20

39.259

95.745

0.376