Detailed information    

insolico Bioinformatically predicted

Overview

Name   addB   Type   Machinery gene
Locus tag   QRD88_RS05285 Genome accession   NZ_CP128114
Coordinates   1040567..1044076 (+) Length   1169 a.a.
NCBI ID   WP_286099019.1    Uniprot ID   -
Organism   Bacillus safensis strain G6S3     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1035567..1049076
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QRD88_RS05255 (QRD88_05250) lepB 1036084..1036596 (+) 513 WP_286099018.1 signal peptidase I -
  QRD88_RS05260 (QRD88_05255) - 1036636..1037781 (-) 1146 WP_024425494.1 AbrB family transcriptional regulator -
  QRD88_RS05265 (QRD88_05260) - 1038048..1038470 (+) 423 WP_024425495.1 BlaI/MecI/CopY family transcriptional regulator -
  QRD88_RS05270 (QRD88_05265) - 1038463..1039302 (+) 840 WP_024425496.1 M56 family metallopeptidase -
  QRD88_RS05275 (QRD88_05270) - 1039360..1039770 (+) 411 WP_024425497.1 DoxX family protein -
  QRD88_RS05280 (QRD88_05275) - 1039968..1040414 (-) 447 WP_044334155.1 ferritin-like domain-containing protein -
  QRD88_RS05285 (QRD88_05280) addB 1040567..1044076 (+) 3510 WP_286099019.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  QRD88_RS05290 (QRD88_05285) addA 1044054..1047758 (+) 3705 WP_286099020.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  QRD88_RS05295 (QRD88_05290) sbcD 1047785..1048960 (+) 1176 WP_024426531.1 exonuclease subunit SbcD -

Sequence

Protein


Download         Length: 1169 a.a.        Molecular weight: 134917.05 Da        Isoelectric Point: 5.3929

>NTDB_id=845946 QRD88_RS05285 WP_286099019.1 1040567..1044076(+) (addB) [Bacillus safensis strain G6S3]
MEIQFLAGRSGSGKTTAILEEIKEQLRLDPLGPPIIFLVPDQMTFLMEYELAKTSEAGGMIRAKVFSFTRLAWTILQQTG
GANRQFVTSTGIQMLLRKVIEEEKEKFKVFKKASDKPGFVEQIEKTMAEFKRYCMLPEDIEKISLSSMHSEYTEERRAAE
KLHDLHVLYQQMEQHLQDEYVHSEDYLTLLTQQIPLSEEIKGAHIYIDGFYQFTPQQLLVIEQLLLHAEKITAAFTVDRS
YHDKQPNELDLFRMTGKTYFQLYQLAKECGAAISEHILERNNRHLHTPDLAYLEQQYEQRPVQPYQEVTPHLTVSKSASK
RAEIEGVAREILHLVREEGLRLRDISIVARHVDDYKDTLKEVFRDYDIPFFIDGNESMQYHPLIELIRSSLDVIKGNWRY
EAVFRCVKTEFLFPLELAKNKAREQADQLENYCIAYGVKGERWTNGSRFHYRRFQSLDEDFGQTDQEIEMEQMLNDVKEW
IAPPLFQLQKRLKKAQKVKDMVEAVYVFLEEIQVPDKLEKARLEAEEAGRLAEAMQHGQVWDAVIQLMDEFVEMLGEEEL
SFPLFQQMMDTGLASLKFALIPPSLDQVFIGSMDLSRMYQVKCMFIIGVNDGVIPARPSDESVLSEDDREWLKRAGAELA
ETGKERLLDEQFLIYQALSSPSHHLYLSYSVSDEEGRSLLPSTLIKYCQELMPNHEHALYVLDPEQLEDKEQLKFVANEH
VSLSYTVSQLQQWLNQYPISGVWWSVYNYLMTSSHRDVSKNILSSLFFTNRAKRLKPNVIKELYGDHIQGSVSRMEKFNA
CAFSHFASHGLKLKDRQFYKLEAPDIGQLFHSALKHISDTLVEQKKDWKNLTKDDCVNYSRHAIEQLAPRLQKEILLSSN
RHAYIKEKLQKILIRVSSILSEHARVSGFSPVGLELGFGGQGPLPPFTFQLKNGCTMELVGRIDRVDKAEGSKGLLLRIV
DYKSSEKGLDLAEVYYGLALQMLTYLDLTITYSKEWLGVEATPAGILYFHIHDPLIQAPIPLAEDEIEQEIFKKFKMKGL
LLEDVEAVKLMDQTLESGRSQVIQAGLKKDGSFRSDSAVLSEDHFHILTQHVRRTFEEAGERITNGEVAINPYKLKDQTP
CRFCSFKSICQFDESIEDNEFRVLSSEKDDVVIDRIKKEGDQYANTKTE

Nucleotide


Download         Length: 3510 bp        

>NTDB_id=845946 QRD88_RS05285 WP_286099019.1 1040567..1044076(+) (addB) [Bacillus safensis strain G6S3]
GTGGAGATTCAGTTTTTAGCAGGCAGATCGGGGAGTGGAAAAACGACTGCAATCTTAGAGGAAATCAAAGAACAGCTTCG
GCTTGATCCGTTGGGTCCGCCAATCATTTTTTTAGTCCCGGATCAAATGACATTCTTAATGGAATACGAGCTTGCAAAAA
CGTCTGAAGCTGGCGGGATGATCAGGGCCAAAGTATTTAGTTTCACGCGTCTTGCTTGGACTATTTTACAGCAGACTGGC
GGAGCGAACCGGCAATTTGTGACAAGCACAGGAATTCAAATGCTTTTAAGAAAAGTGATTGAAGAGGAGAAAGAAAAGTT
TAAGGTATTCAAAAAAGCGAGTGATAAGCCGGGGTTTGTGGAGCAAATCGAAAAGACGATGGCTGAGTTCAAGAGATACT
GCATGCTGCCGGAGGATATTGAAAAAATTTCATTATCGAGCATGCATTCAGAGTATACAGAAGAAAGACGGGCAGCCGAG
AAATTGCATGATCTGCATGTTCTTTATCAGCAGATGGAGCAGCATTTACAAGATGAATATGTGCACTCAGAAGACTATTT
GACACTTCTCACGCAGCAAATTCCTTTATCTGAAGAAATAAAAGGAGCACACATTTATATAGATGGTTTTTATCAATTTA
CACCACAGCAGCTCCTCGTCATTGAACAGCTTCTGCTGCATGCTGAGAAAATAACGGCTGCTTTCACAGTGGATCGTTCC
TATCATGATAAGCAGCCAAATGAACTTGATTTATTTCGTATGACAGGTAAAACGTATTTCCAGCTCTATCAGCTTGCCAA
AGAGTGCGGAGCGGCCATTTCTGAGCACATTTTGGAGAGAAATAACCGTCATCTTCATACGCCGGATCTTGCTTATTTAG
AACAGCAATATGAACAGAGACCGGTGCAGCCATATCAGGAAGTCACTCCTCATCTCACAGTATCTAAAAGTGCAAGTAAA
CGAGCTGAAATTGAGGGAGTAGCGAGAGAAATTCTTCATTTAGTGAGAGAAGAAGGACTTAGACTGCGAGATATTTCGAT
TGTAGCGCGGCATGTAGACGACTATAAAGATACGTTAAAAGAGGTTTTTCGAGATTACGATATTCCTTTTTTTATTGATG
GAAATGAATCGATGCAGTATCATCCGCTCATTGAATTAATTCGTTCGAGCTTGGATGTCATAAAAGGAAACTGGCGGTAT
GAGGCCGTGTTTCGATGCGTGAAGACAGAGTTCTTATTTCCTCTCGAACTTGCCAAAAACAAAGCGAGAGAACAGGCAGA
TCAGCTTGAAAACTACTGTATTGCTTATGGTGTAAAAGGAGAACGCTGGACAAACGGTTCCCGTTTCCATTACAGGCGGT
TCCAATCCTTAGATGAGGATTTTGGACAAACAGATCAAGAAATTGAAATGGAACAGATGCTGAATGATGTAAAGGAATGG
ATCGCACCTCCGCTTTTCCAATTGCAAAAAAGGCTGAAAAAAGCGCAAAAGGTAAAAGACATGGTAGAGGCAGTCTATGT
CTTTTTAGAGGAAATACAAGTGCCGGATAAACTTGAAAAGGCTAGGCTGGAAGCTGAAGAAGCAGGTAGATTAGCTGAAG
CCATGCAGCATGGACAAGTATGGGATGCTGTTATTCAATTAATGGATGAATTTGTTGAAATGCTCGGTGAGGAGGAGCTC
TCATTTCCTTTATTCCAACAGATGATGGATACAGGATTGGCCTCTTTAAAATTCGCTTTAATTCCGCCTTCACTCGACCA
AGTATTTATCGGAAGTATGGATTTATCCAGAATGTATCAAGTGAAGTGTATGTTTATCATTGGGGTAAATGATGGCGTTA
TTCCCGCACGCCCTTCTGACGAAAGTGTATTGTCTGAGGATGACAGGGAATGGTTAAAACGAGCAGGAGCAGAGCTGGCA
GAGACAGGAAAGGAACGGCTGCTAGATGAGCAATTTTTAATTTACCAGGCGCTGTCGAGTCCATCTCATCATTTGTATCT
GTCTTATTCAGTGTCAGATGAAGAGGGGCGCTCTCTATTGCCTTCGACTCTTATCAAGTATTGTCAGGAGCTTATGCCAA
ATCATGAGCATGCTTTGTATGTGTTAGACCCGGAACAGCTGGAGGACAAGGAGCAATTAAAATTTGTAGCGAATGAGCAT
GTCTCGTTATCGTATACTGTCTCGCAGCTGCAGCAATGGCTCAACCAATATCCCATTAGTGGTGTATGGTGGAGTGTCTA
TAATTATTTGATGACGTCATCTCATCGGGATGTATCAAAAAACATCTTGTCGAGTTTGTTTTTTACAAATCGAGCGAAAC
GCTTAAAACCAAACGTCATAAAAGAACTTTATGGTGATCACATTCAAGGCAGTGTTTCAAGAATGGAGAAATTCAATGCG
TGTGCATTTTCTCACTTTGCTTCCCACGGGTTAAAACTAAAGGACCGGCAATTTTATAAATTGGAAGCACCCGATATCGG
CCAGCTGTTTCACTCGGCCTTAAAGCATATTTCAGATACGCTTGTTGAACAAAAAAAGGATTGGAAAAACCTAACGAAGG
ATGACTGTGTCAACTATTCAAGACATGCAATCGAACAGCTTGCACCGCGTCTTCAAAAGGAAATCCTATTAAGCTCTAAC
CGGCATGCTTATATTAAAGAAAAGCTCCAGAAAATCTTGATTCGTGTCTCTTCTATATTAAGTGAGCATGCAAGAGTGAG
TGGATTTTCTCCCGTTGGGCTTGAGTTAGGCTTTGGCGGCCAAGGACCACTTCCGCCGTTTACTTTTCAATTGAAAAATG
GCTGCACGATGGAGCTTGTTGGAAGAATTGACCGGGTGGATAAAGCAGAAGGTTCAAAAGGGCTGCTGTTGAGAATCGTC
GACTACAAATCGAGTGAGAAAGGTCTTGATCTAGCAGAAGTATACTATGGGCTGGCTTTGCAAATGCTCACATACTTAGA
CCTGACCATTACGTATTCGAAGGAGTGGCTTGGTGTAGAAGCAACACCAGCTGGCATTTTATATTTCCATATTCATGATC
CGCTCATTCAAGCACCGATTCCGCTTGCAGAGGATGAAATTGAGCAGGAAATATTCAAGAAATTTAAAATGAAAGGTCTA
TTGCTTGAAGATGTAGAAGCGGTAAAGCTAATGGATCAAACGCTTGAATCAGGAAGATCACAAGTCATTCAAGCCGGCTT
GAAAAAAGATGGGTCTTTCCGTTCTGACTCTGCTGTTTTAAGTGAAGATCATTTTCATATACTCACACAGCATGTTCGGC
GCACGTTTGAAGAGGCAGGCGAAAGAATTACAAATGGCGAGGTAGCCATTAATCCATATAAATTGAAGGATCAAACACCA
TGCCGCTTTTGTTCATTTAAGTCTATTTGCCAATTTGATGAATCGATAGAAGATAATGAATTTAGAGTGCTTTCATCTGA
AAAGGATGATGTTGTGATAGACCGGATCAAAAAAGAAGGGGATCAGTATGCAAATACCAAAACCGAATAA

Domains

Predicted by InterproScan.

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(790-1131)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addB Bacillus subtilis subsp. subtilis str. 168

65.978

99.316

0.655