Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   QR297_RS02550 Genome accession   NZ_CP127845
Coordinates   552713..553123 (+) Length   136 a.a.
NCBI ID   WP_047934243.1    Uniprot ID   -
Organism   Pseudomonas shirazica strain HY376     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 547713..558123
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QR297_RS02520 (QR297_02520) - 548136..548567 (-) 432 WP_015268860.1 hypothetical protein -
  QR297_RS02525 (QR297_02525) - 548654..549361 (+) 708 WP_309672388.1 energy-coupling factor ABC transporter permease -
  QR297_RS02530 (QR297_02530) yacG 549599..549799 (-) 201 WP_003260624.1 DNA gyrase inhibitor YacG -
  QR297_RS02535 (QR297_02535) coaE 549796..550419 (-) 624 WP_100413056.1 dephospho-CoA kinase -
  QR297_RS02540 (QR297_02540) pilD 550419..551285 (-) 867 WP_102084225.1 A24 family peptidase Machinery gene
  QR297_RS02545 (QR297_02545) - 551288..552496 (-) 1209 WP_309672393.1 type II secretion system F family protein -
  QR297_RS02550 (QR297_02550) pilA 552713..553123 (+) 411 WP_047934243.1 pilin Machinery gene
  QR297_RS02560 (QR297_02560) - 553931..554224 (+) 294 WP_309672395.1 hypothetical protein -
  QR297_RS02565 (QR297_02565) - 554507..555487 (+) 981 WP_309673826.1 integrase domain-containing protein -
  QR297_RS02570 (QR297_02570) - 555484..555771 (+) 288 WP_309672397.1 hypothetical protein -
  QR297_RS02575 (QR297_02575) - 556232..556948 (+) 717 WP_309672399.1 SLATT domain-containing protein -

Sequence

Protein


Download         Length: 136 a.a.        Molecular weight: 13783.04 Da        Isoelectric Point: 7.0812

>NTDB_id=844650 QR297_RS02550 WP_047934243.1 552713..553123(+) (pilA) [Pseudomonas shirazica strain HY376]
MKGQRGITLIELMIVVAIIGILATIALPMYTNHQSRSKAAAGLLEISALKTAMDLRLNDGKDVPDVAALGGQPATAHCAI
TASGKAADGTGSIVCTLVDAPANALGKALTLTRSASGWACTTDIEEDLAPKGCKAP

Nucleotide


Download         Length: 411 bp        

>NTDB_id=844650 QR297_RS02550 WP_047934243.1 552713..553123(+) (pilA) [Pseudomonas shirazica strain HY376]
ATGAAAGGGCAACGCGGTATCACCCTGATCGAACTGATGATCGTCGTCGCGATCATCGGCATTCTGGCGACCATCGCCTT
ACCGATGTACACCAACCACCAGTCACGCTCCAAGGCCGCGGCCGGGTTGCTGGAGATCAGCGCGCTGAAGACCGCGATGG
ACCTGCGGTTGAACGACGGGAAGGATGTGCCGGATGTGGCCGCGTTGGGTGGCCAGCCGGCGACGGCGCACTGTGCGATC
ACGGCTAGCGGCAAGGCGGCGGATGGCACTGGGAGCATCGTTTGCACGCTGGTGGATGCGCCGGCCAATGCGCTGGGCAA
GGCATTGACCCTGACGCGTTCGGCTAGTGGGTGGGCGTGTACGACCGACATCGAGGAAGACCTGGCGCCGAAGGGGTGCA
AGGCGCCTTGA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-27)

Pilin (bacterial filament) (Pilin)

(35-134)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Pseudomonas aeruginosa PAK

43.137

100

0.485
  pilA Acinetobacter baumannii strain A118

44.604

100

0.456
  pilA/pilA1 Eikenella corrodens VA1

37.58

100

0.434
  comP Acinetobacter baylyi ADP1

39.456

100

0.426
  pilA/pilAII Pseudomonas stutzeri DSM 10701

42.963

99.265

0.426
  pilA/pilAI Pseudomonas stutzeri DSM 10701

42.647

100

0.426
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

37.063

100

0.39
  pilA Vibrio cholerae strain A1552

37.063

100

0.39
  pilA Vibrio cholerae C6706

37.063

100

0.39
  pilA Vibrio parahaemolyticus RIMD 2210633

38.462

95.588

0.368