Detailed information NTG0844335

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: QQ412_RS22225
• Genome accession: NZ_CP127514
• Coordinates: 4458247..4459047 (+)
• Length: 266 a.a.
• NCBI ID: WP_004191960.1
• Uniprot ID: A0AAJ5B1F9
• Organism: Klebsiella pneumoniae strain ER22701.4
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4453247..4464047

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
QQ412_RS22190 (QQ412_22165)	gspH	4453403..4453918 (+)	516	WP_166689880.1	type II secretion system minor pseudopilin GspH	-
QQ412_RS22195 (QQ412_22170)	gspI	4453915..4454280 (+)	366	WP_002889025.1	type II secretion system minor pseudopilin GspI	-
QQ412_RS22200 (QQ412_22175)	gspJ	4454277..4454873 (+)	597	WP_032433846.1	type II secretion system minor pseudopilin GspJ	-
QQ412_RS22205 (QQ412_22180)	gspK	4454870..4455856 (+)	987	WP_077255453.1	type II secretion system minor pseudopilin GspK	-
QQ412_RS22210 (QQ412_22185)	gspL	4455843..4457036 (+)	1194	WP_032416685.1	type II secretion system protein GspL	-
QQ412_RS22215 (QQ412_22190)	-	4457029..4457514 (+)	486	WP_004145896.1	type II secretion system protein M	-
QQ412_RS22220 (QQ412_22195)	-	4457504..4458250 (+)	747	WP_004145897.1	type II secretion system protein N	-
QQ412_RS22225 (QQ412_22200)	pilD	4458247..4459047 (+)	801	WP_004191960.1	prepilin peptidase	Machinery gene
QQ412_RS22230 (QQ412_22205)	hrpB	4459095..4461524 (-)	2430	WP_032433845.1	ATP-dependent helicase HrpB	-
QQ412_RS22235 (QQ412_22210)	thpR	4461597..4462133 (+)	537	WP_004145901.1	RNA 2',3'-cyclic phosphodiesterase	-
QQ412_RS22240 (QQ412_22215)	sfsA	4462133..4462849 (+)	717	WP_002888848.1	DNA/RNA nuclease SfsA	-
QQ412_RS22245 (QQ412_22220)	dksA	4463012..4463467 (+)	456	WP_002888845.1	RNA polymerase-binding protein DksA	-

Sequence

Protein

• Length: 266 a.a.
• Molecular weight: 29403.04 Da
• Isoelectric Point: 8.8894

>NTDB_id=844335 QQ412_RS22225 WP_004191960.1 4458247..4459047(+) (pilD) [Klebsiella pneumoniae strain ER22701.4]
MTTLAALSLHFPFVWYGFLLLFGLALGSFYNVVIYRLPRMLTQTADDERITLSTPGSSCPQCRQPIAWRDNIPLLSFLWL
GRRARCCQAPIAWSYPLTELATGLLFILAGALLAPGLPLAGGLVLLSFLLILARIDARTQLLPDRLTLPLLWAGLLFNLN
EVYIALPDAVAGAMAGYLALWSVYWLFRLLTGKEALGYGDFKLLAALGAWCGWQVLPQVLLLASASGLVWTLLQRLWTRQ
SLQQPLAFGPWLALAGGGIFLWQQMI

Nucleotide

• Length: 801 bp

>NTDB_id=844335 QQ412_RS22225 WP_004191960.1 4458247..4459047(+) (pilD) [Klebsiella pneumoniae strain ER22701.4]
ATGACGACACTAGCCGCGTTATCGCTGCACTTTCCCTTTGTCTGGTATGGCTTTCTGCTGCTGTTCGGCCTGGCGCTGGG
CAGCTTTTACAATGTGGTGATCTATCGTCTGCCGCGCATGTTGACCCAGACCGCGGACGACGAGCGGATAACCCTCAGCA
CCCCGGGATCTTCCTGCCCGCAGTGCCGCCAGCCGATCGCCTGGCGGGACAATATCCCGCTGCTCAGTTTCCTCTGGCTG
GGCCGTCGCGCCCGCTGCTGTCAGGCGCCCATCGCGTGGAGCTATCCGCTGACCGAGCTGGCCACCGGCCTGCTGTTTAT
CCTCGCCGGAGCTTTGCTGGCGCCGGGCCTGCCGCTGGCGGGCGGCCTGGTGCTGCTCTCGTTCCTGCTGATACTGGCGC
GGATTGACGCCCGGACCCAGCTGCTGCCCGATCGCCTGACTCTGCCCCTGCTGTGGGCCGGGCTGCTGTTCAATCTTAAC
GAGGTGTACATTGCCCTGCCGGATGCGGTCGCCGGGGCGATGGCCGGTTATCTGGCGCTGTGGTCGGTCTACTGGCTGTT
CCGCCTGCTGACCGGGAAAGAGGCGCTGGGCTATGGCGATTTTAAGCTGCTGGCGGCGCTGGGCGCCTGGTGCGGCTGGC
AGGTTCTGCCGCAGGTGCTGTTGCTCGCTTCGGCCAGCGGGCTGGTGTGGACCCTACTGCAACGCCTGTGGACCCGGCAG
TCGCTGCAGCAGCCGCTGGCCTTCGGCCCCTGGCTGGCGCTGGCCGGCGGGGGTATTTTTCTCTGGCAGCAGATGATCTA
G

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-109)

• Type IV leader peptidase family (Peptidase_A24) (123-231)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	49.057	99.624	0.489
pilD	Vibrio campbellii strain DS40M4	46.545	100	0.481
pilD	Acinetobacter nosocomialis M2	46.388	98.872	0.459
pilD	Acinetobacter baumannii D1279779	45.627	98.872	0.451
pilD	Neisseria gonorrhoeae MS11	43.922	95.865	0.421