Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilS   Type   Regulator
Locus tag   QMK58_RS25640 Genome accession   NZ_CP125377
Coordinates   5717946..5719532 (+) Length   528 a.a.
NCBI ID   WP_053162512.1    Uniprot ID   -
Organism   Pseudomonas sp. P8_241     
Function   regulate pilin expression (predicted from homology)   
Competence regulation

Genomic Context

Location: 5712946..5724532
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QMK58_RS25620 (QMK58_25620) pgeF 5714740..5715465 (-) 726 WP_053162505.1 peptidoglycan editing factor PgeF -
  QMK58_RS25625 (QMK58_25625) rluD 5715462..5716424 (-) 963 WP_053162507.1 23S rRNA pseudouridine(1911/1915/1917) synthase RluD -
  QMK58_RS25630 (QMK58_25630) - 5716576..5717598 (+) 1023 WP_320395600.1 outer membrane protein assembly factor BamD -
  QMK58_RS25635 (QMK58_25635) - 5717723..5717956 (+) 234 WP_082344551.1 PP0621 family protein -
  QMK58_RS25640 (QMK58_25640) pilS 5717946..5719532 (+) 1587 WP_053162512.1 sensor histidine kinase Regulator
  QMK58_RS25645 (QMK58_25645) pilR 5719529..5720866 (+) 1338 WP_053162514.1 sigma-54-dependent transcriptional regulator Regulator
  QMK58_RS25650 (QMK58_25650) thiO 5720957..5722057 (-) 1101 WP_320395601.1 glycine oxidase ThiO -
  QMK58_RS25655 (QMK58_25655) - 5722115..5722516 (-) 402 WP_053162518.1 type IV pilin protein -
  QMK58_RS25660 (QMK58_25660) - 5722531..5722818 (-) 288 WP_256220755.1 hypothetical protein -
  QMK58_RS25665 (QMK58_25665) - 5722934..5723398 (-) 465 WP_053162522.1 PilX N-terminal domain-containing pilus assembly protein -
  QMK58_RS25670 (QMK58_25670) - 5723409..5724116 (-) 708 WP_053162524.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -

Sequence

Protein


Download         Length: 528 a.a.        Molecular weight: 58500.43 Da        Isoelectric Point: 8.3692

>NTDB_id=831598 QMK58_RS25640 WP_053162512.1 5717946..5719532(+) (pilS) [Pseudomonas sp. P8_241]
MTAERISPGGKQARRLLRLYHLYRLTIGITLVLVSSNIDTPLLTSANDALLRRGSWLYLVLNILLVALPGNTRRHGQLFS
LALVDVLLLSGLFYAAGGVASAIGNLLVVSVAISNTLLRRRIGLLIAAVGALGIVGFSFLLSLTHAASANDYLQAGTLGT
LCFVAALLVQGLTRRVEVSESLAEQRASEVAGLETLNALILQRMRTGILVLDEQRYVQLANHSALTLLGCALIQSRRIDD
CAPALVERLQRWLDNPVRRPQSLKIESNGLELQPSFIALDQSPQHQTLVFLEDLAHIAQQAQQLKLAGLGRLTASISHEI
RNPLGAISHAAQLLRESEELNAADRRLTQIIQDHSQRMNRVIENVLQLSRREKTVPQRLDLKPWLEQFVADAREEIAQNQ
KIHLNIGAGNFKTLMDPNQLNQILGNLLNNAWRHNTRDQENAKVWLDLFIDRDTQLPTLDILDNGPGVAPELQTHLFEPF
FTSSPQGTGLGLYLSRELCESNQAGLDFKARQEGGCFRIIFAHERKQI

Nucleotide


Download         Length: 1587 bp        

>NTDB_id=831598 QMK58_RS25640 WP_053162512.1 5717946..5719532(+) (pilS) [Pseudomonas sp. P8_241]
GTGACCGCTGAGAGAATCAGTCCCGGCGGCAAACAGGCCCGCCGCCTGCTGCGCCTCTATCATCTCTACCGCTTGACCAT
CGGCATTACCCTGGTGCTGGTCTCCAGTAACATTGATACCCCGCTGCTGACATCTGCCAACGATGCTTTATTGCGCCGTG
GCAGTTGGCTGTATCTGGTCCTGAATATTTTGCTGGTGGCGCTTCCTGGAAACACGCGCCGGCACGGGCAATTGTTCAGT
CTGGCGCTGGTCGACGTCCTGTTGCTGAGCGGCCTGTTCTACGCCGCCGGTGGTGTGGCCAGCGCCATCGGCAATCTTCT
GGTCGTATCGGTGGCCATCAGCAATACCCTGTTGCGAAGGCGCATCGGCCTATTGATTGCAGCTGTCGGTGCGCTTGGTA
TCGTCGGCTTCAGCTTTCTCCTGAGCCTCACCCATGCGGCCAGCGCCAATGATTACCTGCAAGCGGGCACGCTCGGCACC
CTGTGCTTTGTAGCAGCACTGTTGGTGCAAGGCCTGACCCGGCGCGTCGAAGTCAGCGAAAGCCTGGCCGAACAGCGGGC
CAGCGAAGTGGCTGGCCTCGAAACCCTCAACGCCCTGATCCTGCAACGCATGCGCACCGGCATTCTCGTGCTTGATGAAC
AGCGATACGTGCAACTGGCCAATCACAGCGCCCTGACCTTGCTCGGTTGTGCCCTCATCCAAAGCCGCCGAATCGACGAC
TGCGCTCCTGCCTTGGTCGAGCGTTTGCAACGCTGGCTCGACAATCCGGTTCGCCGCCCCCAGAGCCTTAAGATCGAAAG
TAACGGCCTGGAATTGCAACCTAGCTTCATTGCACTGGACCAGAGCCCACAACACCAGACACTGGTCTTTCTCGAAGACC
TTGCCCACATCGCGCAACAGGCTCAGCAACTGAAACTCGCAGGGTTGGGTCGCCTGACCGCGAGCATCTCCCATGAAATC
CGCAATCCGCTGGGTGCCATCAGTCATGCTGCACAACTGTTACGTGAATCAGAGGAACTGAACGCTGCCGATCGACGTCT
GACGCAGATTATTCAAGATCACTCCCAACGCATGAATCGAGTCATCGAAAACGTCCTGCAACTGTCTCGCCGCGAAAAAA
CTGTGCCGCAACGGCTCGACTTGAAGCCATGGCTGGAACAATTCGTGGCCGATGCCCGCGAAGAAATAGCCCAAAACCAG
AAGATTCACTTGAACATCGGTGCCGGCAATTTCAAAACCCTGATGGACCCGAATCAGTTGAACCAGATTCTCGGTAATTT
GTTAAACAATGCGTGGCGCCATAACACCCGTGACCAGGAAAACGCGAAAGTCTGGCTTGATCTGTTCATCGACCGCGACA
CCCAACTGCCAACCCTCGATATCCTCGACAACGGCCCCGGCGTAGCGCCGGAGCTTCAAACGCATCTGTTCGAACCTTTC
TTCACTTCCAGCCCACAAGGCACAGGCCTGGGGCTTTATCTGTCCCGTGAGCTGTGCGAAAGCAATCAGGCCGGCCTGGA
CTTCAAGGCACGCCAAGAGGGCGGCTGCTTTCGCATCATCTTTGCTCACGAACGGAAACAAATTTGA

Domains

Predicted by InterProScan.

Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase (HATPase_c)

(416-519)

His Kinase A (phospho-acceptor) domain (HisKA)

(309-373)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilS Pseudomonas aeruginosa PAK

56.629

100

0.566