Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   R5024_RS24980 Genome accession   NZ_CP137510
Coordinates   5338727..5340451 (+) Length   574 a.a.
NCBI ID   WP_317939711.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain HPA0310     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5333727..5345451
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  R5024_RS24960 (R5024_24960) nadC 5335542..5336390 (+) 849 WP_003122081.1 carboxylating nicotinate-nucleotide diphosphorylase -
  R5024_RS24970 (R5024_24970) - 5336571..5337956 (-) 1386 WP_003122080.1 O-antigen ligase family protein -
  R5024_RS24975 (R5024_24975) pilA 5338032..5338496 (-) 465 WP_003122079.1 pilin Machinery gene
  R5024_RS24980 (R5024_24980) pilB 5338727..5340451 (+) 1725 WP_317939711.1 type IV-A pilus assembly ATPase PilB Machinery gene
  R5024_RS24985 (R5024_24985) - 5340451..5341613 (+) 1163 WP_317939660.1 IS3-like element IS222 family transposase -
  R5024_RS24990 (R5024_24990) pilC 5341670..5342887 (+) 1218 WP_003107299.1 type II secretion system F family protein Machinery gene
  R5024_RS24995 (R5024_24995) pilD 5342888..5343760 (+) 873 WP_003107301.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  R5024_RS25000 (R5024_25000) coaE 5343757..5344368 (+) 612 WP_003094654.1 dephospho-CoA kinase -
  R5024_RS25005 (R5024_25005) yacG 5344365..5344565 (+) 201 WP_003094656.1 DNA gyrase inhibitor YacG -
  R5024_RS25010 (R5024_25010) - 5344602..5344811 (-) 210 WP_003094660.1 hypothetical protein -

Sequence

Protein


Download         Length: 574 a.a.        Molecular weight: 63093.11 Da        Isoelectric Point: 5.4503

>NTDB_id=821687 R5024_RS24980 WP_317939711.1 5338727..5340451(+) (pilB) [Pseudomonas aeruginosa strain HPA0310]
MNDSTPLSGLAKQLVLNELLDEKTARQAQVQAARNKQSLVSHLVQAKLVQSRALVEVAAEQFGIAYCDLNSLDRESIPKD
VISERLVRQHRVLPLWKRGNKLFIGLSDPTNHQAVTDIQFSTGLTTEALLVEDDKLGVAIEKLYEGAMDALGDLGDIDLD
GVDVGKAESASQDDGGDDAEDAPVVRFVNKMLLDAIRGGSSDLHFEPYEKTYRVRFRTDGMLHEVAKPPIQLASRISARL
KVMAGLDISERRKPQDGRIKMRVSKTKSIDFRVNTLPTLWGEKIVMRILDSSSAQMGIDALGYEEDQKELYLAALKQPQG
MILVTGPTGSGKTVSLYTGLNILNTTDINISTAEDPVEINLEGINQVNVNPRQGMDFSQALRAFLRQDPDVIMVGEIRDL
ETAEIAIKAAQTGHMVMSTLHTNSAAETLTRLLNMGVPAFNLATSVNLIIAQRLARKLCSHCKKEHDVPKETLLHEGFPE
ELIGTFKLYSPVGCENCKGGYKGRVGIYEVVKNTPALQRIIMEEGNSIEIAEQARKEGFNDLRTSGLLKAMQGCSGLLIP
DTDLGENPRRERGV

Nucleotide


Download         Length: 1725 bp        

>NTDB_id=821687 R5024_RS24980 WP_317939711.1 5338727..5340451(+) (pilB) [Pseudomonas aeruginosa strain HPA0310]
ATGAACGATTCCACGCCTCTCAGCGGCCTTGCCAAGCAACTGGTCCTGAACGAACTCCTCGACGAAAAGACCGCCCGCCA
GGCCCAGGTGCAGGCCGCGCGCAACAAGCAGTCGCTGGTCAGCCACCTGGTCCAGGCCAAGCTGGTGCAGAGCCGGGCGT
TGGTCGAGGTGGCCGCCGAGCAGTTCGGCATCGCCTATTGCGACCTGAACAGCCTGGATCGGGAAAGCATTCCGAAGGAT
GTCATCAGCGAGCGCCTGGTCCGCCAGCACCGTGTGTTGCCGCTGTGGAAACGCGGCAACAAACTGTTCATCGGTCTCTC
CGACCCGACCAACCACCAGGCGGTCACCGATATCCAGTTCAGCACCGGTCTCACCACCGAAGCCCTGCTGGTGGAGGACG
ACAAGCTCGGCGTAGCCATCGAGAAGCTCTACGAGGGCGCTATGGACGCTCTCGGCGACCTCGGTGACATCGATCTCGAC
GGGGTCGATGTCGGCAAGGCGGAAAGCGCCAGCCAGGACGACGGCGGCGACGATGCCGAAGATGCCCCGGTGGTGCGCTT
CGTCAACAAGATGCTGCTCGACGCCATCCGCGGCGGCTCTTCCGACCTGCACTTCGAGCCCTACGAAAAGACCTACCGGG
TGCGCTTCCGTACCGACGGCATGCTCCACGAAGTGGCCAAGCCGCCGATCCAGTTGGCCAGCCGCATTTCCGCGCGACTC
AAGGTAATGGCCGGCCTGGATATCTCCGAACGGCGCAAGCCGCAGGACGGGCGGATCAAGATGCGCGTGTCGAAGACCAA
GTCGATCGACTTCCGCGTCAACACCCTGCCGACCCTGTGGGGCGAGAAGATCGTGATGCGGATCCTCGACTCCTCCAGCG
CGCAGATGGGCATCGACGCACTGGGCTACGAAGAGGACCAGAAGGAACTCTACCTGGCCGCGCTCAAGCAGCCACAGGGC
ATGATCCTGGTCACCGGCCCCACCGGCTCGGGCAAGACGGTTTCGCTGTACACCGGTCTGAACATCCTCAATACCACCGA
CATCAACATCTCCACCGCTGAAGACCCGGTGGAGATCAACCTGGAAGGCATCAACCAGGTCAACGTCAACCCGCGTCAGG
GCATGGACTTCTCCCAGGCGCTGCGCGCCTTCCTGCGCCAGGACCCGGACGTGATCATGGTCGGCGAGATCCGCGATCTG
GAGACTGCCGAGATCGCCATCAAGGCGGCGCAGACCGGGCATATGGTGATGTCCACCCTGCACACCAACAGCGCCGCCGA
GACCCTGACCCGCCTGCTGAACATGGGCGTGCCGGCGTTCAACCTGGCGACCTCGGTGAACCTGATCATCGCCCAGCGCC
TTGCGCGAAAACTCTGTTCGCACTGCAAGAAAGAACACGACGTGCCGAAGGAAACCCTGCTTCACGAAGGTTTCCCGGAA
GAGCTGATCGGCACCTTCAAGCTGTATTCGCCTGTAGGCTGCGAAAACTGCAAAGGCGGTTACAAAGGTCGCGTCGGTAT
TTATGAAGTGGTTAAAAACACCCCCGCCCTGCAGCGGATTATCATGGAAGAAGGCAACTCCATCGAGATCGCCGAGCAAG
CCCGCAAAGAAGGCTTCAACGATCTGCGCACCTCAGGCCTGCTGAAAGCCATGCAGGGATGTAGTGGTCTACTGATCCCG
GACACCGATTTAGGCGAAAATCCTCGCCGTGAGAGAGGTGTCTGA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(190-457)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-146)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

57.041

97.735

0.557
  pilB Acinetobacter baylyi ADP1

56.452

97.213

0.549
  pilB Legionella pneumophila strain ERS1305867

53.584

97.213

0.521
  pilB Vibrio cholerae strain A1552

50.995

96.341

0.491
  pilF Neisseria gonorrhoeae MS11

49.55

96.69

0.479
  pilB Vibrio parahaemolyticus RIMD 2210633

51.613

91.812

0.474
  pilB Vibrio campbellii strain DS40M4

50.664

91.812

0.465
  pilB/pilB1 Synechocystis sp. PCC 6803

36.765

100

0.392
  pilF Thermus thermophilus HB27

37.986

98.606

0.375
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.122

94.599

0.361