Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   QBS25_RS20765 Genome accession   NZ_CP121859
Coordinates   4381528..4382667 (+) Length   379 a.a.
NCBI ID   WP_019446654.1    Uniprot ID   -
Organism   Aeromonas veronii strain LOID 15995     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4376528..4387667
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QBS25_RS20735 - 4376598..4376978 (-) 381 WP_005337641.1 diacylglycerol kinase -
  QBS25_RS20740 - 4377123..4378184 (+) 1062 WP_064336455.1 3-deoxy-7-phosphoheptulonate synthase -
  QBS25_RS20745 - 4378301..4379098 (-) 798 WP_274160364.1 ATP-binding cassette domain-containing protein -
  QBS25_RS20750 btuC 4379164..4380171 (-) 1008 WP_043825636.1 vitamin B12 ABC transporter permease BtuC -
  QBS25_RS20755 - 4380269..4381000 (-) 732 WP_043825634.1 transporter substrate-binding domain-containing protein -
  QBS25_RS20760 - 4381113..4381469 (+) 357 WP_005337656.1 DMT family protein -
  QBS25_RS20765 pilU 4381528..4382667 (+) 1140 WP_019446654.1 type IVa pilus ATPase TapW Machinery gene
  QBS25_RS20770 - 4382762..4383211 (-) 450 WP_149152581.1 isoprenylcysteine carboxylmethyltransferase family protein -
  QBS25_RS20775 alr 4383385..4384611 (+) 1227 WP_274160279.1 alanine racemase -
  QBS25_RS20780 - 4384677..4385621 (-) 945 WP_005353134.1 TerC family protein -
  QBS25_RS20785 oppF 4385924..4386919 (-) 996 WP_005337666.1 murein tripeptide/oligopeptide ABC transporter ATP binding protein OppF -

Sequence

Protein


Download         Length: 379 a.a.        Molecular weight: 42238.37 Da        Isoelectric Point: 6.6011

>NTDB_id=814868 QBS25_RS20765 WP_019446654.1 4381528..4382667(+) (pilU) [Aeromonas veronii strain LOID 15995]
MELRDMLQILAKQDGSDLYLSTGAPPCAKFNGGLRPLNETPLEPGEVARIAAEIMDGEQKLQFDKELEMNLAISLPQIGR
FRVNIFKQRNEVSLVARNIKTEIPRFEDLKLPPVLLDTIMEKRGLVLFVGGTGSGKSTSLAALIDHRNRNSGGHIITIED
PVEFVHRHRKSIINQREVGVDTRSFHAALKNTLRQAPDVILIGEIRDRETMEHALAFSETGHLAISTLHANNANQALDRI
INFFPEERRPQLLNDLGNNLKAFVSQRLVKTTDGGRRAAVEIMLGTHTIRDMIKRGEFGGLKEVMEKSKALGMVTFDSAL
FDLVVEGVIEEEEAVKNADSANNLRLKIKLWKEKGQIASSSDATGWSLEPTKDEKGDFF

Nucleotide


Download         Length: 1140 bp        

>NTDB_id=814868 QBS25_RS20765 WP_019446654.1 4381528..4382667(+) (pilU) [Aeromonas veronii strain LOID 15995]
ATGGAATTGCGAGACATGTTACAGATCCTGGCCAAGCAGGATGGCTCAGACCTCTATCTGTCGACCGGCGCGCCCCCCTG
CGCCAAGTTTAACGGCGGCCTGCGCCCCCTCAACGAAACGCCGCTGGAGCCGGGTGAAGTGGCGCGGATCGCCGCCGAGA
TCATGGATGGCGAGCAGAAACTGCAATTTGACAAGGAGCTGGAGATGAACCTTGCCATCTCCCTGCCCCAGATCGGCCGC
TTTCGGGTCAACATCTTCAAGCAGCGCAACGAAGTCTCTCTGGTGGCCCGCAACATCAAGACCGAAATCCCCCGCTTTGA
AGATCTCAAACTCCCGCCTGTGCTGCTCGACACCATCATGGAGAAACGCGGTCTGGTACTGTTTGTCGGCGGCACCGGCT
CGGGTAAATCTACCTCGCTGGCGGCGCTTATCGACCACCGCAACCGCAACAGCGGCGGCCACATCATTACCATCGAGGAT
CCGGTGGAGTTCGTTCATCGCCACCGCAAGAGCATCATCAACCAGCGGGAGGTTGGCGTGGATACCCGCAGCTTCCACGC
CGCCCTGAAAAACACCCTGCGTCAGGCGCCGGACGTCATTCTTATCGGCGAGATCCGCGATCGCGAGACCATGGAGCATG
CGCTGGCCTTCTCCGAAACCGGCCACCTTGCCATCTCCACCCTGCACGCCAACAACGCCAACCAGGCGCTGGATCGCATC
ATCAACTTCTTCCCCGAGGAGCGCCGCCCCCAGCTGCTCAACGATCTGGGCAACAACCTCAAGGCTTTTGTCTCCCAGCG
GCTGGTGAAAACCACCGATGGCGGCCGCCGGGCCGCAGTGGAGATCATGCTTGGCACCCACACCATCCGCGACATGATCA
AGCGCGGCGAATTTGGTGGTCTCAAAGAGGTGATGGAAAAATCCAAGGCCCTCGGCATGGTCACCTTCGACAGTGCCCTG
TTTGATCTGGTAGTGGAAGGGGTAATTGAGGAAGAAGAGGCGGTGAAAAACGCCGACTCGGCCAACAACCTGCGCCTCAA
GATCAAGCTGTGGAAGGAGAAGGGGCAGATTGCTAGCAGCAGCGATGCCACCGGCTGGAGTCTCGAACCCACCAAGGACG
AGAAGGGCGATTTTTTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(113-248)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

54.474

100

0.546
  pilU Acinetobacter baylyi ADP1

52.958

93.668

0.496
  pilU Vibrio cholerae strain A1552

50.286

92.348

0.464
  pilT Legionella pneumophila strain Lp02

42.216

88.127

0.372
  pilT Legionella pneumophila strain ERS1305867

42.216

88.127

0.372
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.593

89.446

0.372
  pilT Pseudomonas aeruginosa PAK

40.988

90.765

0.372
  pilT Pseudomonas stutzeri DSM 10701

40.407

90.765

0.367
  pilT Acinetobacter baumannii D1279779

41.317

88.127

0.364
  pilT Acinetobacter baumannii strain A118

41.317

88.127

0.364
  pilT Acinetobacter nosocomialis M2

41.317

88.127

0.364
  pilT Vibrio cholerae strain A1552

41.441

87.863

0.364
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.441

87.863

0.364