Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   QBS06_RS20335 Genome accession   NZ_CP121798
Coordinates   4225592..4226731 (+) Length   379 a.a.
NCBI ID   WP_011706550.1    Uniprot ID   A0AAI9Q2R8
Organism   Aeromonas hydrophila strain YURI 17     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4220592..4231731
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QBS06_RS20305 - 4220899..4221963 (+) 1065 WP_011706545.1 3-deoxy-7-phosphoheptulonate synthase -
  QBS06_RS20310 aac(6') 4221970..4222428 (+) 459 WP_041217812.1 aminoglycoside 6'-N-acetyltransferase -
  QBS06_RS20315 - 4222450..4223235 (-) 786 WP_052143537.1 ATP-binding cassette domain-containing protein -
  QBS06_RS20320 btuC 4223222..4224229 (-) 1008 WP_024946382.1 vitamin B12 ABC transporter permease BtuC -
  QBS06_RS20325 - 4224330..4225061 (-) 732 WP_029300033.1 transporter substrate-binding domain-containing protein -
  QBS06_RS20330 - 4225175..4225531 (+) 357 WP_005302630.1 DMT family protein -
  QBS06_RS20335 pilU 4225592..4226731 (+) 1140 WP_011706550.1 type IVa pilus ATPase TapW Machinery gene
  QBS06_RS20340 glgC 4227014..4228285 (+) 1272 WP_279451078.1 glucose-1-phosphate adenylyltransferase -
  QBS06_RS20345 - 4228421..4228900 (+) 480 WP_279451079.1 WbuC family cupin fold metalloprotein -
  QBS06_RS20350 - 4229025..4229267 (-) 243 WP_011706553.1 FeoC-like transcriptional regulator -
  QBS06_RS20355 feoB 4229264..4231537 (-) 2274 WP_279451081.1 Fe(2+) transporter permease subunit FeoB -

Sequence

Protein


Download         Length: 379 a.a.        Molecular weight: 42264.32 Da        Isoelectric Point: 6.4290

>NTDB_id=814293 QBS06_RS20335 WP_011706550.1 4225592..4226731(+) (pilU) [Aeromonas hydrophila strain YURI 17]
MELRDMLQILAKQDGSDLYLSTGAPPCAKFNGGLRPLSETPLEPGEVARIADAIMDGEQKQQFERELEMNLAISLPQIGR
FRINIFKQRNEVSLVARNIKTEIPRFEDLKLPPVLLDTIMEKRGLVLFVGGTGSGKSTSLAALIDHRNRNSGGHIITIED
PVEFVHRHRKSIINQREVGVDTRSFHAALKNTLRQAPDVILIGEIRDRETMEHALAFSETGHLAISTLHANNANQALDRI
INFFPEERRPQLLNDLGNNLKAFVSQRLVKTSDGGRRAAVEIMLGTHTIRDMIKRGEFGGLKEVMEKSKALGMVTFDSAL
FDLVVEGVIDEEEAVKNADSANNLRLKIKLWKEKGQIASSSDATGWSLEPTKDEKDDLF

Nucleotide


Download         Length: 1140 bp        

>NTDB_id=814293 QBS06_RS20335 WP_011706550.1 4225592..4226731(+) (pilU) [Aeromonas hydrophila strain YURI 17]
ATGGAATTGCGAGACATGCTACAGATCCTGGCCAAGCAGGATGGCTCCGACCTCTATCTGTCGACCGGCGCCCCCCCTTG
CGCCAAGTTCAACGGCGGCCTGCGTCCGCTCAGTGAAACACCGCTCGAGCCCGGCGAGGTGGCCAGAATAGCCGACGCCA
TCATGGACGGCGAGCAAAAGCAGCAGTTCGAGCGGGAGCTGGAGATGAACCTCGCCATCTCGCTGCCCCAGATCGGCCGC
TTTCGGATCAACATCTTCAAGCAGCGCAACGAAGTGTCGCTGGTGGCGCGCAACATCAAGACCGAGATCCCCCGCTTCGA
GGATCTGAAACTGCCCCCCGTACTGCTCGACACCATCATGGAGAAGCGCGGCCTGGTGCTGTTCGTCGGCGGTACCGGCT
CGGGCAAATCCACTTCGCTCGCGGCCCTCATCGACCATCGCAACCGCAACAGCGGCGGCCACATCATCACCATCGAGGAT
CCGGTGGAGTTCGTGCACCGCCACCGCAAGAGCATCATCAACCAGCGCGAGGTGGGGGTGGATACCCGCAGCTTCCACGC
CGCCCTCAAGAACACCCTGCGCCAGGCGCCGGACGTGATCCTCATCGGTGAAATTCGCGACCGCGAGACCATGGAGCATG
CGCTGGCCTTCTCCGAGACCGGCCACCTGGCGATTTCCACCCTGCACGCCAACAACGCCAACCAGGCGCTGGACCGCATC
ATCAACTTCTTCCCGGAAGAGCGCCGACCCCAGCTGCTCAACGACCTTGGCAACAACCTCAAGGCGTTTGTCTCCCAGCG
GCTGGTCAAAACCAGTGACGGTGGCCGCCGGGCGGCGGTGGAGATCATGCTGGGCACCCACACCATCCGCGACATGATCA
AACGCGGCGAATTTGGCGGCCTCAAGGAGGTGATGGAGAAATCCAAGGCCCTCGGCATGGTCACCTTCGACAGCGCCCTG
TTCGATCTGGTGGTCGAGGGGGTGATCGACGAGGAAGAGGCGGTGAAGAACGCCGACTCGGCCAACAACCTGCGCCTCAA
GATCAAGCTGTGGAAGGAGAAGGGCCAGATCGCCAGCAGCAGCGATGCCACCGGCTGGAGCCTCGAGCCCACCAAGGATG
AAAAAGACGATCTGTTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(114-248)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

54.133

98.945

0.536
  pilU Acinetobacter baylyi ADP1

53.521

93.668

0.501
  pilU Vibrio cholerae strain A1552

50.286

92.348

0.464
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.076

93.14

0.383
  pilT Pseudomonas aeruginosa PAK

40.698

90.765

0.369
  pilT Legionella pneumophila strain ERS1305867

41.916

88.127

0.369
  pilT Legionella pneumophila strain Lp02

41.916

88.127

0.369
  pilT Pseudomonas stutzeri DSM 10701

40.116

90.765

0.364
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.441

87.863

0.364
  pilT Vibrio cholerae strain A1552

41.441

87.863

0.364
  pilT Acinetobacter baumannii D1279779

41.018

88.127

0.361
  pilT Acinetobacter baumannii strain A118

41.018

88.127

0.361
  pilT Acinetobacter nosocomialis M2

41.018

88.127

0.361