Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   RTE57_RS04615 Genome accession   NZ_CP135957
Coordinates   928392..929372 (+) Length   326 a.a.
NCBI ID   WP_001527643.1    Uniprot ID   -
Organism   Escherichia coli strain MS1657     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 923392..934372
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RTE57_RS04585 (RTE57_04585) yggM 923993..925000 (+) 1008 WP_107131032.1 DUF1202 family protein -
  RTE57_RS04590 (RTE57_04590) hemW 925069..926205 (-) 1137 WP_317728968.1 radical SAM family heme chaperone HemW -
  RTE57_RS04595 (RTE57_04595) rdgB 926198..926791 (-) 594 WP_001174738.1 XTP/dITP diphosphatase -
  RTE57_RS04600 (RTE57_04600) yggU 926799..927089 (-) 291 WP_001277214.1 DUF167 family protein YggU -
  RTE57_RS04605 (RTE57_04605) yggT 927086..927652 (-) 567 WP_001094831.1 osmotic shock tolerance protein YggT -
  RTE57_RS04610 (RTE57_04610) yggS 927670..928374 (-) 705 WP_000997795.1 pyridoxal phosphate homeostasis protein -
  RTE57_RS04615 (RTE57_04615) pilT 928392..929372 (+) 981 WP_001527643.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  RTE57_RS04620 (RTE57_04620) ruvX 929546..929962 (-) 417 WP_000017106.1 Holliday junction resolvase RuvX -
  RTE57_RS04625 (RTE57_04625) yqgE 929962..930525 (-) 564 WP_001053178.1 YqgE/AlgH family protein -
  RTE57_RS04630 (RTE57_04630) gshB 930634..931584 (-) 951 WP_000593260.1 glutathione synthase -
  RTE57_RS04635 (RTE57_04635) rsmE 931597..932328 (-) 732 WP_001222509.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  RTE57_RS04640 (RTE57_04640) endA 932408..933115 (-) 708 WP_001305312.1 deoxyribonuclease I -
  RTE57_RS04645 (RTE57_04645) yggI 933210..933707 (-) 498 WP_000858396.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 36002.17 Da        Isoelectric Point: 5.7995

>NTDB_id=812381 RTE57_RS04615 WP_001527643.1 928392..929372(+) (pilT) [Escherichia coli strain MS1657]
MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFEAPDVEELLREWLDDDQRAILLENGQLDFAVSLAENQRLRG
SAFAQRQGISLALRLLPSHCPQLEQLGAPPVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
YLYTSQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQSVERLVDS
FPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVALFELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMITFQQSYQQR
VGEGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=812381 RTE57_RS04615 WP_001527643.1 928392..929372(+) (pilT) [Escherichia coli strain MS1657]
ATGAATATGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGCAGCGCCTGGCCCGCACG
ATGGCGTATTCGCGGGAGAATGGAAGCTGCGCCGTTTGAGGCGCCGGACGTCGAAGAGCTACTGCGGGAGTGGCTGGATG
ACGATCAGCGGGCAATATTGCTGGAGAATGGTCAGCTGGATTTTGCTGTGTCGCTGGCGGAAAACCAGCGATTGCGCGGC
AGTGCGTTCGCACAACGGCAAGGTATTTCTCTGGCGTTACGGCTGTTACCTTCGCACTGCCCGCAGCTCGAACAGCTTGG
CGCACCACCGGTATTGCCGGAATTACTCAAGAGCGAGAATGGCCTGATTCTGGTGACGGGGGCGACGGGGAGTGGCAAAT
CTACCACGCTGGCGGCGATGGTTGGCTATCTCAATCAACATGCCGATGCGCATATTCTGACGCTGGAAGATCCTGTGGAA
TATCTCTATACCAGTCAGCGATGTTTGATCCAGCAGCGGGAAATTGGTTTGCACTGTATGACGTTCGCATCGGGATTGCG
GGCCGCATTGCGGGAAGATCCTGATGTGATTTTGCTCGGAGAGCTGCGTGATAGCGAGACAATCCGTCTGGCGCTGACGG
CGGCAGAAACCGGGCATCTGGTGCTGGCAACATTACATACGCGTGGTGCGGCGCAGTCAGTTGAGCGACTGGTGGATTCA
TTTCCTGCGCAGGAAAAAGATCCCGTGCGTAATCAACTGGCAGGTAGTTTACGGGCAGTGTTGTCACAAAAACTGGAAGT
GGATAAACAGGAAGGACGCGTGGCGCTGTTTGAATTACTGATTAACACACCCGCGGTGGGGAATTTGATTCGTGAAGGAA
AAACCCACCAGTTGCCGCATGTTATTCAAACCGGGCAGCAGGTGGGGATGATAACGTTTCAGCAGAGTTATCAGCAGCGG
GTGGGGGAAGGGCGTTTGTGA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(62-239)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.541

100

0.497
  pilT Vibrio cholerae strain A1552

49.541

100

0.497
  pilT Neisseria meningitidis 8013

48.78

100

0.491
  pilT Neisseria gonorrhoeae MS11

48.476

100

0.488
  pilT Acinetobacter baumannii strain A118

46.789

100

0.469
  pilT Acinetobacter baumannii D1279779

46.789

100

0.469
  pilT Acinetobacter nosocomialis M2

46.789

100

0.469
  pilT Pseudomonas aeruginosa PAK

46.483

100

0.466
  pilT Acinetobacter baylyi ADP1

46.483

100

0.466
  pilT Pseudomonas stutzeri DSM 10701

46.177

100

0.463
  pilT Legionella pneumophila strain ERS1305867

45.566

100

0.457
  pilT Legionella pneumophila strain Lp02

45.566

100

0.457
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.767

97.546

0.417
  pilU Vibrio cholerae strain A1552

39.21

100

0.396
  pilU Pseudomonas stutzeri DSM 10701

37.273

100

0.377
  pilU Acinetobacter baylyi ADP1

36.646

98.773

0.362