Detailed information NTG0810444

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: P8T43_RS04540
• Genome accession: NZ_CP121145
• Coordinates: 971283..972083 (-)
• Length: 266 a.a.
• NCBI ID: WP_032414527.1
• Uniprot ID: -
• Organism: Klebsiella pneumoniae strain UHD-56_PH
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 966283..977083

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
P8T43_RS04520 (P8T43_04520)	dksA	966863..967318 (-)	456	WP_002888845.1	RNA polymerase-binding protein DksA	-
P8T43_RS04525 (P8T43_04525)	sfsA	967481..968197 (-)	717	WP_004177390.1	DNA/RNA nuclease SfsA	-
P8T43_RS04530 (P8T43_04530)	thpR	968197..968733 (-)	537	WP_032414524.1	RNA 2',3'-cyclic phosphodiesterase	-
P8T43_RS04535 (P8T43_04535)	hrpB	968806..971235 (+)	2430	WP_032414526.1	ATP-dependent helicase HrpB	-
P8T43_RS04540 (P8T43_04540)	pilD	971283..972083 (-)	801	WP_032414527.1	A24 family peptidase	Machinery gene
P8T43_RS04545 (P8T43_04545)	-	972080..972826 (-)	747	WP_009309263.1	type II secretion system protein N	-
P8T43_RS04550 (P8T43_04550)	-	972816..973301 (-)	486	WP_004145896.1	type II secretion system protein M	-
P8T43_RS04555 (P8T43_04555)	gspL	973294..974487 (-)	1194	WP_004191957.1	type II secretion system protein GspL	-
P8T43_RS04560 (P8T43_04560)	gspK	974474..975460 (-)	987	WP_077254427.1	type II secretion system minor pseudopilin GspK	-
P8T43_RS04565 (P8T43_04565)	gspJ	975457..976053 (-)	597	WP_004145893.1	type II secretion system minor pseudopilin GspJ	-
P8T43_RS04570 (P8T43_04570)	gspI	976050..976415 (-)	366	WP_002889025.1	type II secretion system minor pseudopilin GspI	-
P8T43_RS04575 (P8T43_04575)	gspH	976412..976927 (-)	516	WP_032414529.1	type II secretion system minor pseudopilin GspH	-

Sequence

Protein

• Length: 266 a.a.
• Molecular weight: 29419.04 Da
• Isoelectric Point: 8.8894

>NTDB_id=810444 P8T43_RS04540 WP_032414527.1 971283..972083(-) (pilD) [Klebsiella pneumoniae strain UHD-56_PH]
MTTLAALSLHFPFVWYGFLLLFGLALGSFYNVVIYRLPRMLTQTADDERITLSTPGSSCPQCRQPISWRDNIPLLSFLWL
GRRARCCQAPIAWSYPLTELATGLLFILAGALLAPGLPLAGGLVLLSFLLILARIDARTQLLPDRLTLPLLWAGLLFNLN
EVYIALPDAVAGAMAGYLALWSVYWLFRLLTGKEALGYGDFKLLAALGAWCGWQVLPQVLLLASASGLVWTLLQRLWTRQ
SLQQPLAFGPWLALAGGGIFLWQQMI

Nucleotide

• Length: 801 bp

>NTDB_id=810444 P8T43_RS04540 WP_032414527.1 971283..972083(-) (pilD) [Klebsiella pneumoniae strain UHD-56_PH]
ATGACGACACTAGCCGCGTTATCGCTGCACTTTCCCTTTGTCTGGTATGGCTTTCTGCTGCTGTTCGGCCTGGCGCTGGG
CAGCTTTTACAATGTGGTGATCTATCGTCTGCCGCGCATGTTGACCCAGACCGCGGACGACGAGCGGATAACCCTCAGCA
CCCCGGGCTCTTCCTGCCCGCAGTGCCGCCAGCCGATCTCCTGGCGGGACAATATCCCGCTGCTCAGTTTCCTCTGGCTG
GGCCGTCGCGCCCGCTGCTGTCAGGCGCCCATCGCGTGGAGCTATCCGCTGACCGAGCTGGCCACCGGCCTGCTGTTTAT
CCTCGCCGGAGCTTTGCTGGCGCCGGGCCTGCCGCTGGCGGGCGGCCTGGTGCTGCTCTCGTTCCTGCTGATACTGGCGC
GGATTGACGCCCGGACCCAACTGCTGCCCGATCGCCTGACTCTGCCCCTGCTGTGGGCCGGGCTGCTGTTCAATCTTAAC
GAGGTGTACATTGCCCTGCCGGATGCGGTCGCCGGGGCGATGGCCGGTTATCTGGCGCTGTGGTCGGTCTACTGGCTGTT
CCGCCTGCTGACCGGGAAAGAGGCGCTGGGCTATGGCGATTTTAAGCTGCTGGCGGCGCTGGGCGCCTGGTGCGGCTGGC
AGGTTCTGCCGCAGGTGCTGTTGCTCGCTTCGGCCAGCGGGCTGGTGTGGACCCTACTGCAACGCCTGTGGACCCGGCAG
TCGCTGCAGCAGCCGCTGGCCTTCGGCCCCTGGCTGGCGCTGGCCGGCGGGGGTATTTTTCTCTGGCAGCAGATGATCTA
G

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-109)

• Type IV leader peptidase family (Peptidase_A24) (123-231)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	49.057	99.624	0.489
pilD	Vibrio campbellii strain DS40M4	46.545	100	0.481
pilD	Acinetobacter nosocomialis M2	46.388	98.872	0.459
pilD	Acinetobacter baumannii D1279779	45.627	98.872	0.451
pilD	Neisseria gonorrhoeae MS11	43.922	95.865	0.421