Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   RPM47_RS09515 Genome accession   NZ_CP135098
Coordinates   2039870..2040493 (+) Length   207 a.a.
NCBI ID   WP_003103268.1    Uniprot ID   G3XD51
Organism   Pseudomonas aeruginosa strain Paer2090     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2034870..2045493
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RPM47_RS09500 (RPM47_09500) - 2035560..2038028 (-) 2469 WP_003114576.1 penicillin-binding protein 1A -
  RPM47_RS09505 (RPM47_09505) pilM 2038213..2039277 (+) 1065 WP_003110888.1 type IV pilus assembly protein PilM Machinery gene
  RPM47_RS09510 (RPM47_09510) pilN 2039277..2039873 (+) 597 WP_003095839.1 type 4a pilus biogenesis protein PilN Machinery gene
  RPM47_RS09515 (RPM47_09515) pilO 2039870..2040493 (+) 624 WP_003103268.1 type 4a pilus biogenesis protein PilO Machinery gene
  RPM47_RS09520 (RPM47_09520) comL/pilP 2040490..2041014 (+) 525 WP_003095836.1 type 4a pilus biogenesis lipoprotein PilP Machinery gene
  RPM47_RS09525 (RPM47_09525) pilQ 2041068..2043212 (+) 2145 WP_003146401.1 type 4a pilus secretin PilQ Machinery gene
  RPM47_RS09530 (RPM47_09530) aroK 2043224..2043742 (+) 519 WP_003095833.1 shikimate kinase AroK -
  RPM47_RS09535 (RPM47_09535) aroB 2043792..2044898 (+) 1107 WP_003162482.1 3-dehydroquinate synthase -

Sequence

Protein


Download         Length: 207 a.a.        Molecular weight: 22819.26 Da        Isoelectric Point: 4.7787

>NTDB_id=809525 RPM47_RS09515 WP_003103268.1 2039870..2040493(+) (pilO) [Pseudomonas aeruginosa strain Paer2090]
MSLASSLESLRKIDINDLDLNNIGSWPAAVKVIVCVLLTAAVLALGYNFHLSDMQAQLEQQAAEEETLKQQFSTKAFQAA
NLEAYKAQMKEMEESFGALLRQLPSDTEVPGLLEDITRTGLGSGLEFEEIKLLPEVAQQFYIELPIQISVVGGYHDLATF
VSGVSSLPRIVTLHDFEIKPVAPGSTSKLRMSILAKTYRYNDKGLKK

Nucleotide


Download         Length: 624 bp        

>NTDB_id=809525 RPM47_RS09515 WP_003103268.1 2039870..2040493(+) (pilO) [Pseudomonas aeruginosa strain Paer2090]
ATGAGTCTGGCCAGTTCCCTGGAAAGTCTGCGCAAGATCGATATCAACGATCTCGACCTGAACAACATCGGTTCCTGGCC
GGCGGCGGTCAAGGTCATCGTCTGCGTGCTGCTGACCGCGGCGGTCCTGGCGCTGGGCTACAACTTCCATCTGAGTGACA
TGCAGGCTCAGCTCGAACAGCAGGCCGCGGAAGAGGAGACGCTCAAGCAGCAGTTCTCCACCAAGGCCTTCCAGGCCGCG
AACCTGGAAGCCTACAAGGCACAGATGAAGGAGATGGAAGAGTCCTTTGGCGCCTTGCTGCGGCAGTTGCCCAGCGACAC
CGAGGTACCCGGGCTGCTCGAGGACATCACTCGTACCGGCCTGGGCAGCGGCCTGGAGTTCGAGGAAATCAAGCTGCTTC
CCGAGGTTGCCCAGCAGTTCTACATCGAGCTGCCGATCCAGATCAGCGTGGTCGGCGGCTACCACGACCTGGCGACCTTC
GTCAGCGGCGTGTCCAGCCTGCCGCGGATCGTCACCCTGCATGACTTCGAGATCAAGCCGGTCGCGCCCGGCAGCACGTC
CAAGCTGCGCATGAGCATCCTGGCCAAGACCTATCGCTACAACGACAAGGGGCTGAAGAAATGA

Domains

Predicted by InterProScan.

Pilus assembly protein, PilO (PilO)

(58-202)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB G3XD51

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Legionella pneumophila strain ERS1305867

46.114

93.237

0.43
  pilO Acinetobacter baumannii D1279779

43.979

92.271

0.406
  comO/pilO Acinetobacter baylyi ADP1

43.455

92.271

0.401