Detailed information NTG0809216

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: P6N29_RS16905
• Genome accession: NZ_CP120901
• Coordinates: 3575911..3576708 (-)
• Length: 265 a.a.
• NCBI ID: WP_015585126.1
• Uniprot ID: A0A855FAW5
• Organism: Raoultella ornithinolytica strain GY22PK022
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3570911..3581708

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
P6N29_RS16885 (P6N29_16855)	dksA	3571516..3571971 (-)	456	WP_004858018.1	RNA polymerase-binding protein DksA	-
P6N29_RS16890 (P6N29_16860)	sfsA	3572136..3572852 (-)	717	WP_004858020.1	DNA/RNA nuclease SfsA	-
P6N29_RS16895 (P6N29_16865)	thpR	3572852..3573388 (-)	537	WP_004858022.1	RNA 2',3'-cyclic phosphodiesterase	-
P6N29_RS16900 (P6N29_16870)	hrpB	3573461..3575890 (+)	2430	WP_044348317.1	ATP-dependent helicase HrpB	-
P6N29_RS16905 (P6N29_16875)	pilD	3575911..3576708 (-)	798	WP_015585126.1	A24 family peptidase	Machinery gene
P6N29_RS16910 (P6N29_16880)	-	3576723..3577463 (-)	741	WP_015585125.1	type II secretion system protein N	-
P6N29_RS16915 (P6N29_16885)	gspM	3577453..3577947 (-)	495	WP_064357507.1	type II secretion system protein GspM	-
P6N29_RS16920 (P6N29_16890)	gspL	3577940..3579142 (-)	1203	WP_204111659.1	type II secretion system protein GspL	-
P6N29_RS16925 (P6N29_16895)	gspK	3579129..3580115 (-)	987	WP_015585122.1	type II secretion system minor pseudopilin GspK	-
P6N29_RS16930 (P6N29_16900)	gspJ	3580112..3580696 (-)	585	WP_015585121.1	type II secretion system minor pseudopilin GspJ	-
P6N29_RS16935 (P6N29_16905)	gspI	3580708..3581073 (-)	366	WP_004858044.1	type II secretion system minor pseudopilin GspI	-
P6N29_RS16940 (P6N29_16910)	gspH	3581070..3581588 (-)	519	WP_015585120.1	type II secretion system minor pseudopilin GspH	-

Sequence

Protein

• Length: 265 a.a.
• Molecular weight: 29285.93 Da
• Isoelectric Point: 8.4762

>NTDB_id=809216 P6N29_RS16905 WP_015585126.1 3575911..3576708(-) (pilD) [Raoultella ornithinolytica strain GY22PK022]
MTLLSAFSLQFPLLWGGLLFIFGLTFGSFFNVVIYRLPLMMQQEESARFNLCVPASFCPQCQRPIIWRDNIPLLSYLSLK
GRARCCQAPISQRYPLTELASGLLFVLAGYLLTPGLPLLGGLILLSTLLVLAIIDGQTQLLPDRLTLPLLWAGLLFNLNG
TFVPLSEAVIGAMTGYLSLWTVYWLFRLLTGKEALGYGDFKLLAALGAWSGWQILPQTLLCASASGLIWTLLQRRITLQS
LDQPLAFGPWLALAGSGLFLWTQTL

Nucleotide

• Length: 798 bp

>NTDB_id=809216 P6N29_RS16905 WP_015585126.1 3575911..3576708(-) (pilD) [Raoultella ornithinolytica strain GY22PK022]
ATGACGCTACTGAGCGCATTTTCGTTGCAGTTCCCCCTGTTATGGGGCGGATTGTTGTTTATTTTCGGCCTGACGTTTGG
CAGCTTTTTTAACGTGGTTATCTACCGTTTGCCGCTGATGATGCAGCAGGAAGAGTCCGCACGATTCAACCTGTGCGTTC
CCGCCTCCTTCTGCCCCCAGTGCCAGCGGCCCATCATCTGGCGGGATAATATTCCGCTGCTCAGCTATCTCTCACTCAAA
GGGCGCGCCCGCTGCTGCCAGGCGCCCATTTCTCAGCGCTATCCGCTGACGGAACTGGCCAGCGGACTGCTCTTTGTGTT
GGCCGGCTATCTGCTGACGCCGGGATTACCGCTGCTGGGGGGCCTGATCCTGCTCTCCACGCTGCTGGTACTGGCGATCA
TCGACGGCCAGACTCAGCTCCTGCCGGATCGGCTGACTCTGCCGCTGCTGTGGGCCGGTCTGCTGTTTAATCTCAACGGC
ACGTTTGTCCCGCTCAGCGAAGCGGTGATCGGCGCCATGACGGGCTATTTATCACTGTGGACGGTTTACTGGCTGTTTCG
CCTGCTGACCGGCAAAGAGGCGCTAGGATACGGTGATTTTAAGCTGCTGGCGGCACTCGGCGCATGGTCCGGCTGGCAGA
TCCTGCCGCAGACGCTGCTGTGCGCCTCGGCCAGCGGCCTGATCTGGACCTTGCTGCAACGGCGGATCACGCTCCAGTCG
CTGGATCAGCCGCTGGCCTTCGGCCCCTGGCTGGCGCTGGCGGGCAGCGGCCTGTTTCTGTGGACTCAGACGCTGTAA

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-109)

• Type IV leader peptidase family (Peptidase_A24) (122-230)

3D structure

• AlphaFold DB: A0A855FAW5

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	48.529	100	0.498
pilD	Vibrio campbellii strain DS40M4	45.221	100	0.464
pilD	Neisseria gonorrhoeae MS11	46.614	94.717	0.442
pilD	Acinetobacter baumannii D1279779	45.276	95.849	0.434
pilD	Acinetobacter nosocomialis M2	44.186	97.358	0.43