Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   RI132_RS00690 Genome accession   NZ_CP134783
Coordinates   142604..144103 (+) Length   499 a.a.
NCBI ID   WP_072616301.1    Uniprot ID   -
Organism   Vibrio vulnificus strain GCU-01     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 137604..149103
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RI132_RS00670 (RI132_00670) hslO 137993..138868 (-) 876 WP_011078947.1 Hsp33 family molecular chaperone HslO -
  RI132_RS00675 (RI132_00675) hslR 138900..139286 (-) 387 WP_011078946.1 ribosome-associated heat shock protein Hsp15 -
  RI132_RS00680 (RI132_00680) gspC 139671..140546 (+) 876 WP_172665345.1 type II secretion system protein GspC -
  RI132_RS00685 (RI132_00685) gspD 140583..142604 (+) 2022 WP_311888325.1 type II secretion system secretin GspD -
  RI132_RS00690 (RI132_00690) pilB/pilB1 142604..144103 (+) 1500 WP_072616301.1 type II secretion system ATPase GspE Machinery gene
  RI132_RS00695 (RI132_00695) gspF 144103..145320 (+) 1218 WP_011078942.1 type II secretion system inner membrane protein GspF -
  RI132_RS00700 (RI132_00700) gspG 145374..145817 (+) 444 WP_011078941.1 type II secretion system major pseudopilin GspG -
  RI132_RS00705 (RI132_00705) gspH 145744..146448 (+) 705 WP_081372415.1 type II secretion system minor pseudopilin GspH -
  RI132_RS00710 (RI132_00710) gspI 146441..146803 (+) 363 WP_011078939.1 type II secretion system minor pseudopilin GspI -
  RI132_RS00715 (RI132_00715) gspJ 146790..147491 (+) 702 WP_072616303.1 type II secretion system minor pseudopilin GspJ -
  RI132_RS00720 (RI132_00720) gspK 147484..148506 (+) 1023 WP_165386729.1 type II secretion system minor pseudopilin GspK -

Sequence

Protein


Download         Length: 499 a.a.        Molecular weight: 55844.17 Da        Isoelectric Point: 6.0826

>NTDB_id=807856 RI132_RS00690 WP_072616301.1 142604..144103(+) (pilB/pilB1) [Vibrio vulnificus strain GCU-01]
MVDILDTAPSYRRLPFSFANRFKMVLEVEHPERPPVLYYVEPLNAQALVEVRRVLKQTFVPQAIAAEAFEKKLTEAYQRD
SSEARQLMEDIGSDDDFFSLAEELPQDEDLLEAEDDAPIIKLINAMLGEAIKEGASDIHIETFEKILSIRFRVDGVLRDV
LSPSRKLAPLLVSRVKVMAKLDIAEKRVPQDGRISLRIGGRAVDVRVSTMPSSHGERVVMRLLDKNATRLDLHSLGMTPV
NHDNFRHLISRPHGIILVTGPTGSGKSTTLYAGLQELNSNERNILTVEDPIEFDIDGIGQTQVNPKVDMTFARGLRAILR
QDPDVVMVGEIRDLETAQIAVQASLTGHLVMSTLHTNTAVGAITRLRDMGIEPFLISSSLLGVLAQRLVRTLCQDCKEPY
EADKEQRKLFGVKKKDALTLYRAKGCEKCNHKGYRGRTGIHELLMVDDKVQELIHAEAGEQAMDKYIREHTPSIRSDGLD
KVLQGVTSLEEVMRVTKES

Nucleotide


Download         Length: 1500 bp        

>NTDB_id=807856 RI132_RS00690 WP_072616301.1 142604..144103(+) (pilB/pilB1) [Vibrio vulnificus strain GCU-01]
ATGGTAGATATACTGGATACGGCGCCATCGTATCGCCGTTTACCGTTTAGTTTTGCTAACCGCTTTAAAATGGTGCTGGA
AGTCGAGCATCCAGAAAGGCCGCCGGTACTTTACTACGTTGAGCCTCTCAATGCGCAGGCGTTAGTTGAAGTCCGTCGTG
TGCTCAAACAGACGTTTGTCCCTCAAGCCATTGCCGCTGAAGCGTTTGAAAAAAAACTGACCGAAGCGTACCAACGCGAC
TCGTCAGAAGCGCGCCAGTTGATGGAAGACATTGGCTCGGATGATGACTTCTTCTCTTTGGCTGAAGAGCTGCCGCAAGA
TGAAGATTTGCTGGAAGCGGAAGACGATGCGCCGATCATCAAATTGATCAACGCCATGTTGGGCGAAGCGATCAAAGAAG
GTGCGTCAGATATTCACATCGAAACGTTCGAGAAAATTCTGTCGATTCGTTTCCGCGTCGATGGCGTTTTGCGCGATGTG
TTGTCTCCGAGCCGCAAACTGGCGCCGCTGTTGGTGTCACGTGTTAAAGTCATGGCCAAACTGGATATTGCAGAAAAACG
CGTGCCACAAGATGGCCGTATTTCACTGCGTATCGGTGGTCGCGCGGTCGATGTGCGTGTTTCGACCATGCCTTCATCAC
ACGGAGAGCGTGTGGTGATGCGTTTGCTGGATAAAAACGCCACTCGTCTTGACCTGCATAGCTTGGGCATGACGCCGGTG
AACCACGACAATTTCCGTCATCTTATCTCGCGCCCTCACGGCATTATCTTGGTCACAGGTCCAACCGGCTCAGGTAAATC
CACCACCTTGTACGCTGGCCTGCAAGAGCTGAACAGCAACGAGCGCAACATCTTAACGGTGGAAGACCCGATCGAATTTG
ATATCGATGGTATCGGCCAAACCCAAGTGAACCCAAAAGTCGACATGACCTTTGCTCGTGGTCTGCGAGCCATTTTGCGT
CAAGACCCAGACGTGGTGATGGTCGGGGAGATTCGTGACTTGGAAACCGCACAAATTGCCGTACAAGCGTCATTGACCGG
TCACTTGGTGATGTCAACCTTGCACACCAACACCGCCGTAGGTGCCATTACCCGTCTGCGTGACATGGGCATTGAGCCCT
TTTTGATCTCTTCTTCACTGCTGGGTGTGTTGGCTCAGCGCCTGGTGCGGACCTTATGCCAAGACTGTAAAGAACCGTAC
GAGGCCGACAAAGAGCAGCGCAAACTGTTTGGCGTGAAGAAGAAAGATGCGCTGACCTTATATCGTGCCAAGGGATGTGA
AAAATGTAACCACAAAGGTTATCGTGGCCGTACGGGTATCCATGAGCTGTTGATGGTAGACGATAAAGTCCAAGAGCTGA
TCCATGCTGAGGCGGGAGAGCAGGCGATGGACAAATACATTCGTGAACATACCCCAAGCATTCGCAGCGACGGTTTAGAC
AAAGTGTTGCAAGGCGTGACCTCACTGGAAGAAGTGATGCGCGTGACCAAGGAATCTTAA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(125-391)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

42.776

100

0.451
  pilF Thermus thermophilus HB27

44.915

94.589

0.425
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.713

98.998

0.423
  pilB Acinetobacter baumannii D1279779

42.292

96.192

0.407
  pilB Legionella pneumophila strain ERS1305867

46.934

84.97

0.399
  pilB Vibrio parahaemolyticus RIMD 2210633

46.348

79.559

0.369
  pilB Vibrio campbellii strain DS40M4

44.853

81.764

0.367
  pilB Vibrio cholerae strain A1552

45.96

79.359

0.365
  pilF Neisseria gonorrhoeae MS11

46.193

78.958

0.365
  pilB Acinetobacter baylyi ADP1

46.41

78.156

0.363