Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   P5638_RS13405 Genome accession   NZ_CP120599
Coordinates   2617445..2617825 (-) Length   126 a.a.
NCBI ID   WP_277722987.1    Uniprot ID   -
Organism   Bacillus safensis strain PRO114     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 2612445..2622825
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  P5638_RS13370 (P5638_13370) - 2613034..2613264 (-) 231 WP_024425132.1 DUF896 domain-containing protein -
  P5638_RS13375 (P5638_13375) - 2613334..2613981 (-) 648 WP_024425131.1 recombinase family protein -
  P5638_RS13380 (P5638_13380) yneA 2613999..2614310 (-) 312 WP_024425130.1 cell division suppressor protein YneA -
  P5638_RS13385 (P5638_13385) lexA 2614469..2615089 (+) 621 WP_024425129.1 transcriptional repressor LexA -
  P5638_RS13390 (P5638_13390) - 2615134..2615580 (-) 447 WP_024425128.1 hypothetical protein -
  P5638_RS13395 (P5638_13395) - 2615876..2616418 (+) 543 WP_024425127.1 IseA DL-endopeptidase inhibitor family protein -
  P5638_RS13400 (P5638_13400) - 2616451..2617299 (-) 849 WP_024425126.1 STAS domain-containing protein -
  P5638_RS13405 (P5638_13405) nucA/comI 2617445..2617825 (-) 381 WP_277722987.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  P5638_RS13410 (P5638_13410) - 2618084..2618992 (-) 909 WP_024425124.1 trypsin-like serine protease -
  P5638_RS13415 (P5638_13415) - 2619185..2622328 (-) 3144 WP_277721961.1 bifunctional cytochrome P450/NADPH--P450 reductase -

Sequence

Protein


Download         Length: 126 a.a.        Molecular weight: 13771.47 Da        Isoelectric Point: 8.5068

>NTDB_id=806515 P5638_RS13405 WP_277722987.1 2617445..2617825(-) (nucA/comI) [Bacillus safensis strain PRO114]
MGTLFGGFGEKQAAKGADRYDHVVQFPKERYPETGSHIQEAIRKGHSDVCTIDRNGADARRQESLKGIPTKPGFDRDEWP
MAVCLEGGKGASVQYVSPPDNRGAGSWVGHQISGFPDGKRILFIVK

Nucleotide


Download         Length: 381 bp        

>NTDB_id=806515 P5638_RS13405 WP_277722987.1 2617445..2617825(-) (nucA/comI) [Bacillus safensis strain PRO114]
ATGGGCACGTTGTTTGGCGGATTTGGAGAGAAGCAGGCAGCAAAAGGGGCTGATCGATATGATCATGTCGTTCAATTTCC
AAAGGAAAGGTACCCTGAAACAGGCAGTCATATTCAAGAAGCCATTCGAAAAGGGCATTCAGATGTGTGTACCATTGACC
GAAATGGAGCAGATGCCCGCAGGCAAGAATCATTAAAAGGAATTCCGACAAAACCTGGCTTTGACCGGGATGAATGGCCA
ATGGCGGTTTGTCTTGAGGGAGGAAAAGGCGCAAGCGTTCAATATGTCAGTCCACCTGATAATAGGGGGGCAGGCTCATG
GGTCGGGCATCAAATCAGCGGGTTTCCTGATGGGAAAAGAATATTATTCATTGTGAAATAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(29-116)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

57.983

94.444

0.548