Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   RG643_RS20755 Genome accession   NZ_CP133753
Coordinates   4429932..4430555 (-) Length   207 a.a.
NCBI ID   WP_003103268.1    Uniprot ID   G3XD51
Organism   Pseudomonas aeruginosa strain ZYPA187     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4424932..4435555
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RG643_RS20735 (RG643_20730) aroB 4425539..4426645 (-) 1107 WP_033989914.1 3-dehydroquinate synthase -
  RG643_RS20740 (RG643_20735) aroK 4426695..4427213 (-) 519 WP_003095833.1 shikimate kinase AroK -
  RG643_RS20745 (RG643_20740) pilQ 4427225..4429357 (-) 2133 WP_033989912.1 type 4a pilus secretin PilQ Machinery gene
  RG643_RS20750 (RG643_20745) comL/pilP 4429411..4429935 (-) 525 WP_003095836.1 type 4a pilus biogenesis lipoprotein PilP Machinery gene
  RG643_RS20755 (RG643_20750) pilO 4429932..4430555 (-) 624 WP_003103268.1 type 4a pilus biogenesis protein PilO Machinery gene
  RG643_RS20760 (RG643_20755) pilN 4430552..4431148 (-) 597 WP_003095839.1 type 4a pilus biogenesis protein PilN Machinery gene
  RG643_RS20765 (RG643_20760) pilM 4431148..4432212 (-) 1065 WP_003110888.1 type IV pilus assembly protein PilM Machinery gene
  RG643_RS20770 (RG643_20765) - 4432397..4434868 (+) 2472 WP_016562439.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 207 a.a.        Molecular weight: 22819.26 Da        Isoelectric Point: 4.7787

>NTDB_id=800803 RG643_RS20755 WP_003103268.1 4429932..4430555(-) (pilO) [Pseudomonas aeruginosa strain ZYPA187]
MSLASSLESLRKIDINDLDLNNIGSWPAAVKVIVCVLLTAAVLALGYNFHLSDMQAQLEQQAAEEETLKQQFSTKAFQAA
NLEAYKAQMKEMEESFGALLRQLPSDTEVPGLLEDITRTGLGSGLEFEEIKLLPEVAQQFYIELPIQISVVGGYHDLATF
VSGVSSLPRIVTLHDFEIKPVAPGSTSKLRMSILAKTYRYNDKGLKK

Nucleotide


Download         Length: 624 bp        

>NTDB_id=800803 RG643_RS20755 WP_003103268.1 4429932..4430555(-) (pilO) [Pseudomonas aeruginosa strain ZYPA187]
ATGAGTCTGGCCAGTTCCCTGGAAAGTCTGCGCAAGATCGATATCAACGATCTCGACCTGAACAACATCGGTTCCTGGCC
GGCGGCGGTCAAGGTCATCGTCTGCGTGCTGCTGACCGCGGCGGTCCTGGCGCTGGGCTACAACTTCCATCTGAGTGACA
TGCAGGCTCAGCTCGAACAGCAGGCCGCGGAAGAGGAGACGCTCAAGCAGCAGTTCTCCACCAAGGCCTTCCAGGCCGCG
AACCTGGAAGCCTACAAGGCACAGATGAAGGAGATGGAAGAGTCCTTTGGCGCCTTGCTGCGGCAGTTGCCCAGCGACAC
CGAGGTACCCGGGCTGCTCGAGGACATCACTCGTACCGGCCTGGGCAGCGGCCTGGAGTTCGAGGAAATCAAGCTGCTTC
CCGAGGTTGCCCAGCAGTTCTACATCGAGCTGCCGATCCAGATCAGCGTGGTCGGCGGCTACCACGACCTGGCGACCTTC
GTCAGCGGCGTGTCCAGCCTGCCGCGGATCGTCACCCTGCATGACTTCGAGATCAAGCCGGTCGCGCCCGGCAGCACGTC
CAAGCTGCGCATGAGCATCCTGGCCAAGACCTATCGCTACAACGACAAGGGGCTGAAGAAATGA

Domains

Predicted by InterProScan.

Pilus assembly protein, PilO (PilO)

(58-202)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB G3XD51

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Legionella pneumophila strain ERS1305867

46.114

93.237

0.43
  pilO Acinetobacter baumannii D1279779

43.979

92.271

0.406
  comO/pilO Acinetobacter baylyi ADP1

43.455

92.271

0.401