Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   P0M07_RS14405 Genome accession   NZ_CP119431
Coordinates   3046541..3047677 (+) Length   378 a.a.
NCBI ID   WP_325770860.1    Uniprot ID   -
Organism   Aeromonas caviae strain K523     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3041541..3052677
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  P0M07_RS14375 (P0M07_14385) - 3041642..3042022 (-) 381 WP_010675195.1 diacylglycerol kinase -
  P0M07_RS14380 (P0M07_14390) - 3042164..3043228 (+) 1065 WP_010675196.1 3-deoxy-7-phosphoheptulonate synthase -
  P0M07_RS14385 (P0M07_14395) - 3043441..3044214 (-) 774 WP_216994206.1 ATP-binding cassette domain-containing protein -
  P0M07_RS14390 (P0M07_14400) btuC 3044201..3045208 (-) 1008 WP_039041213.1 vitamin B12 ABC transporter permease BtuC -
  P0M07_RS14395 (P0M07_14405) - 3045284..3046015 (-) 732 WP_270825441.1 ABC transporter substrate-binding protein -
  P0M07_RS14400 (P0M07_14410) - 3046128..3046484 (+) 357 WP_201985102.1 DMT family protein -
  P0M07_RS14405 (P0M07_14415) pilU 3046541..3047677 (+) 1137 WP_325770860.1 type IVa pilus ATPase TapW Machinery gene
  P0M07_RS14410 (P0M07_14420) - 3047739..3047981 (-) 243 WP_039041210.1 FeoC-like transcriptional regulator -
  P0M07_RS14415 (P0M07_14425) feoB 3048028..3050298 (-) 2271 WP_407160954.1 Fe(2+) transporter permease subunit FeoB -
  P0M07_RS14420 (P0M07_14430) - 3050295..3050522 (-) 228 WP_010675204.1 FeoA family protein -
  P0M07_RS14425 (P0M07_14435) - 3050641..3050970 (+) 330 WP_325770863.1 stress response translation initiation inhibitor YciH -
  P0M07_RS14430 (P0M07_14440) - 3051224..3051781 (+) 558 WP_010675206.1 TMEM165/GDT1 family protein -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 42235.33 Da        Isoelectric Point: 6.6000

>NTDB_id=799287 P0M07_RS14405 WP_325770860.1 3046541..3047677(+) (pilU) [Aeromonas caviae strain K523]
MELRDMLQILAKQDGSDLYLSTGAPPCAKFNGALRPLTQEPLGPGEVARIAYDIMDLEQKQQFEKELEMNLAISLPQIGR
FRVNIFKQRNEVSLVARNIKTEIPRFEDLKLPPVLLDTIMEKRGLVLFVGGTGSGKSTSLAALIDHRNRNSGGHIITIED
PVEFVHRHQKSIINQREVGVDTRSFHAALKNTLRQAPDVILIGEIRDRETMEHALAFSETGHLAISTLHANNANQALDRI
INFFPEERRPQLLNDLGNNLKAFVSQRLVKTTDGGRRAAVEVMLGTHTMRDLIKRGEFGGLKEIMEKSRALGMVTFDSTL
FDLVVEGVIDEEEAVKNADSQNNLRLKIKLWREKGQIGNSAAASGWSLEPTKDEGDLF

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=799287 P0M07_RS14405 WP_325770860.1 3046541..3047677(+) (pilU) [Aeromonas caviae strain K523]
ATGGAATTGAGAGACATGCTGCAGATCCTGGCCAAACAGGACGGTTCCGATCTCTATCTCTCCACCGGCGCCCCCCCCTG
CGCCAAGTTCAACGGCGCCCTGCGCCCCCTCACCCAGGAGCCCCTGGGGCCGGGCGAGGTGGCCCGCATCGCCTACGACA
TCATGGATCTCGAGCAGAAACAGCAGTTCGAGAAAGAGCTGGAGATGAACCTCGCCATCTCCCTGCCCCAGATAGGCCGT
TTTCGGGTCAACATCTTCAAGCAGCGCAATGAAGTCTCCCTGGTGGCGCGCAACATCAAGACCGAGATCCCCCGCTTCGA
GGATCTCAAACTCCCTCCGGTGCTCCTCGACACCATCATGGAGAAGCGGGGTCTGGTGCTCTTCGTCGGCGGCACGGGGT
CCGGCAAATCCACGTCGCTCGCGGCCCTCATCGATCATCGCAACCGCAACAGCGGCGGCCACATCATCACCATCGAGGAT
CCGGTGGAGTTCGTCCATCGCCACCAGAAGAGCATCATCAACCAGCGGGAGGTGGGGGTGGATACCCGCAGCTTCCACGC
GGCCCTGAAGAACACCCTGCGCCAGGCGCCGGACGTCATCCTCATCGGCGAAATCCGCGATCGGGAGACCATGGAGCATG
CTCTCGCCTTCTCGGAGACCGGGCATCTGGCCATCTCCACCCTGCACGCCAACAACGCCAACCAGGCGCTGGATCGCATC
ATCAACTTCTTCCCGGAAGAGCGCAGACCCCAGCTGCTCAACGACCTTGGCAACAACCTCAAGGCCTTTGTCTCCCAGCG
GCTGGTGAAGACCACGGACGGCGGGCGCCGGGCGGCGGTGGAGGTAATGCTAGGAACCCACACGATGCGCGATCTCATCA
AGCGCGGCGAGTTTGGCGGCCTCAAGGAGATCATGGAAAAATCCAGGGCCCTTGGCATGGTGACCTTCGACAGCACTCTG
TTCGATCTGGTGGTCGAGGGGGTGATCGACGAGGAGGAGGCGGTGAAGAACGCGGACTCCCAGAACAACCTGCGCCTCAA
GATCAAACTCTGGCGGGAGAAGGGCCAGATTGGCAACAGCGCGGCGGCGAGCGGCTGGAGCCTGGAGCCCACCAAGGACG
AGGGCGACCTCTTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(114-248)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

54.4

99.206

0.54
  pilU Acinetobacter baylyi ADP1

52.514

94.709

0.497
  pilU Vibrio cholerae strain A1552

49.858

93.386

0.466
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.183

89.683

0.378
  pilT Vibrio cholerae strain A1552

40.456

92.857

0.376
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

40.456

92.857

0.376
  pilT Legionella pneumophila strain ERS1305867

42.216

88.36

0.373
  pilT Legionella pneumophila strain Lp02

42.216

88.36

0.373
  pilT Pseudomonas aeruginosa PAK

40.988

91.005

0.373
  pilT Pseudomonas stutzeri DSM 10701

40.698

91.005

0.37
  pilT Acinetobacter baumannii strain A118

41.317

88.36

0.365
  pilT Acinetobacter nosocomialis M2

41.317

88.36

0.365
  pilT Acinetobacter baumannii D1279779

41.317

88.36

0.365