Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   PX335_RS17470 Genome accession   NZ_CP118933
Coordinates   3635467..3636219 (-) Length   250 a.a.
NCBI ID   WP_017387054.1    Uniprot ID   A0A009HLK6
Organism   Acinetobacter pittii strain ML4     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3630467..3641219
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PX335_RS17450 (PX335_17445) aroB 3631009..3632091 (-) 1083 WP_005067096.1 3-dehydroquinate synthase -
  PX335_RS17455 (PX335_17450) aroK 3632120..3632689 (-) 570 WP_002116987.1 shikimate kinase AroK -
  PX335_RS17460 (PX335_17455) pilQ 3632701..3634866 (-) 2166 WP_017387053.1 type IV pilus secretin PilQ Machinery gene
  PX335_RS17465 (PX335_17460) pilP 3634929..3635456 (-) 528 WP_002116864.1 pilus assembly protein PilP Machinery gene
  PX335_RS17470 (PX335_17465) pilO 3635467..3636219 (-) 753 WP_017387054.1 type 4a pilus biogenesis protein PilO Machinery gene
  PX335_RS17475 (PX335_17470) pilN 3636216..3636857 (-) 642 WP_017387055.1 PilN domain-containing protein Machinery gene
  PX335_RS17480 (PX335_17475) pilM 3636857..3637915 (-) 1059 WP_005067078.1 pilus assembly protein PilM Machinery gene
  PX335_RS17485 (PX335_17480) ponA 3638077..3640638 (+) 2562 WP_017387056.1 penicillin-binding protein PBP1a -

Sequence

Protein


Download         Length: 250 a.a.        Molecular weight: 28227.07 Da        Isoelectric Point: 4.8782

>NTDB_id=796525 PX335_RS17470 WP_017387054.1 3635467..3636219(-) (pilO) [Acinetobacter pittii strain ML4]
MSPDELHELSLEQAPSKKKKITLEKFLQQFNTLDMNSYGSWPLSVKITCWIFIFFAVLALGYFVAIQPKLQAIDNAQAQE
SNLLNEFREKDSKLRNLQQYQLQLQEMQANFNQQLEQLPKETEIPSLVEDINLTGVNSGLKFKNIRLEDEVKQEIFIEQP
ITIEATGDYHAFGAFVSSIAALPRIVTMHDFVVEAAPVKDGKSDIPVLNYSIKAKTYRYMGAVENPEHQEKNASASSVAP
DSSTANTQPK

Nucleotide


Download         Length: 753 bp        

>NTDB_id=796525 PX335_RS17470 WP_017387054.1 3635467..3636219(-) (pilO) [Acinetobacter pittii strain ML4]
ATGAGTCCTGATGAACTTCATGAGCTGTCTTTAGAACAAGCTCCATCTAAGAAAAAAAAGATCACCTTAGAAAAATTTCT
TCAGCAATTTAACACGCTCGATATGAACAGTTATGGCAGCTGGCCATTATCTGTGAAAATTACGTGTTGGATTTTTATTT
TCTTTGCTGTATTGGCTTTAGGGTATTTTGTTGCTATTCAACCAAAGCTACAAGCAATTGATAATGCTCAAGCACAAGAA
AGTAATTTATTAAATGAGTTTCGAGAAAAAGACTCAAAATTACGCAACTTGCAGCAATACCAGCTTCAGCTTCAAGAGAT
GCAAGCCAACTTCAATCAGCAGTTAGAACAATTGCCAAAAGAAACCGAAATTCCAAGTTTAGTTGAAGATATTAACTTAA
CGGGGGTGAATTCTGGTCTGAAGTTTAAAAATATCCGCTTAGAAGATGAAGTAAAGCAGGAAATTTTTATTGAACAGCCT
ATTACTATCGAAGCAACTGGGGATTATCACGCTTTCGGTGCTTTTGTTAGCAGTATTGCGGCATTACCACGTATTGTGAC
AATGCATGATTTTGTTGTTGAAGCTGCTCCTGTTAAGGATGGTAAATCAGATATTCCTGTGCTTAATTATTCTATTAAAG
CAAAAACTTATCGCTATATGGGTGCTGTAGAAAATCCTGAACATCAGGAAAAAAATGCTTCGGCTTCCTCGGTAGCACCT
GATTCTTCTACTGCTAACACACAGCCGAAATAG

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(73-220)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A009HLK6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Acinetobacter baumannii D1279779

83.6

100

0.836
  comO Acinetobacter baylyi ADP1

70

96

0.672