Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   PWW25_RS12715 Genome accession   NZ_CP118584
Coordinates   2705069..2706820 (-) Length   583 a.a.
NCBI ID   WP_005451505.1    Uniprot ID   A0A9X3RXD0
Organism   Vibrio harveyi strain fish5     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2700069..2711820
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PWW25_RS12695 - 2700764..2701603 (-) 840 WP_005451513.1 Dam family site-specific DNA-(adenine-N6)-methyltransferase -
  PWW25_RS12700 - 2701673..2703175 (-) 1503 WP_009697564.1 AAA family ATPase -
  PWW25_RS12705 aroB 2703229..2704329 (-) 1101 WP_038898114.1 3-dehydroquinate synthase -
  PWW25_RS12710 aroK 2704361..2704879 (-) 519 WP_005451507.1 shikimate kinase AroK -
  PWW25_RS12715 pilQ 2705069..2706820 (-) 1752 WP_005451505.1 type IV pilus secretin PilQ family protein Machinery gene
  PWW25_RS12720 pilP 2706855..2707370 (-) 516 WP_005451501.1 pilus assembly protein PilP Machinery gene
  PWW25_RS12725 pilO 2707360..2707953 (-) 594 WP_005451500.1 type 4a pilus biogenesis protein PilO Machinery gene
  PWW25_RS12730 pilN 2707946..2708521 (-) 576 WP_005451499.1 PilN domain-containing protein Machinery gene
  PWW25_RS12735 pilM 2708505..2709521 (-) 1017 WP_029789968.1 type IV pilus assembly protein PilM Machinery gene

Sequence

Protein


Download         Length: 583 a.a.        Molecular weight: 63768.79 Da        Isoelectric Point: 4.5053

>NTDB_id=793667 PWW25_RS12715 WP_005451505.1 2705069..2706820(-) (pilQ) [Vibrio harveyi strain fish5]
MKMRQGLTLTPRKLTGLFSLWLFLLLAPLVAAEEVKSNSLKSIDFRTNKDKDAVIVVELASPAAIVDIKRVQEGLSIDLL
NTSVKDDQLYLLDVKDFSTVVESVEVFRDTSTTRLVAYIDEDYTHDYRLTGRYLEVKVGKLKANEKAPDKSILEKEGKLI
SINFQDIPVRNVLQLIADYNQFNLVVSDSVEGNLTLRLDGVPWQQVLDIILQVKGLDKRVDGNVILVAPKDELDLREKQQ
LEKQKLEQEMGELSSEIIKVNFAKASDIAEMINGEGNISMLSDRGSMTIDERTNSLLIRELPENIQVIREIIESLDIPVK
QVQIEARIVTINEGNMDELGVRWGFTSINGSHTVGGSIENNLATIGLYDGGGGDGDGEDGGGSVGIDDFLNVNLAATSPN
ATSIAFQVAKLGSDTLLDLELSALQQESKAEIISSPRLITTNKKPAYIEQGTEIPYLESSSSGATSVTFKKAVLSLKVTP
QITPDNRLVLDLSVTQDRPGQVVKTGTGEAVAIDTQRIGTQVLVNNGETVVLGGIFQHSITNSVDKVPLLGDLPLLGALF
RRSYENVGKSELLIFVTPKVVIQ

Nucleotide


Download         Length: 1752 bp        

>NTDB_id=793667 PWW25_RS12715 WP_005451505.1 2705069..2706820(-) (pilQ) [Vibrio harveyi strain fish5]
ATGAAAATGAGACAAGGATTAACACTGACACCAAGGAAGCTAACAGGGCTCTTCTCTTTATGGTTGTTTCTACTTTTAGC
CCCTTTAGTCGCAGCGGAAGAAGTAAAGTCTAACTCGCTTAAGAGCATCGACTTCCGTACTAATAAAGATAAAGACGCGG
TTATCGTCGTAGAGCTTGCATCGCCAGCTGCTATTGTTGATATCAAGCGTGTGCAAGAAGGTTTAAGTATCGACTTGCTC
AATACGTCAGTTAAAGACGATCAGCTTTATCTACTTGATGTAAAAGACTTCTCTACAGTGGTAGAGAGTGTTGAGGTTTT
CCGCGATACCTCTACGACGCGTTTGGTCGCTTACATTGATGAAGATTATACCCATGACTATCGCCTCACAGGACGTTACC
TTGAGGTGAAGGTTGGTAAGCTCAAAGCCAACGAAAAAGCACCAGACAAAAGTATTCTGGAAAAAGAAGGCAAGTTGATT
TCGATTAACTTCCAAGATATTCCGGTGCGAAATGTGCTGCAGCTTATTGCCGATTACAACCAGTTTAACCTTGTGGTTTC
AGACTCAGTTGAAGGGAACCTGACCTTGCGTTTGGACGGTGTACCTTGGCAACAAGTGCTGGATATCATCCTTCAAGTCA
AAGGCTTGGATAAGCGTGTAGATGGTAACGTTATTTTGGTTGCTCCGAAGGATGAGCTCGATCTGCGCGAGAAGCAACAA
CTAGAAAAACAAAAGCTTGAACAAGAAATGGGTGAACTTTCTTCTGAAATTATCAAAGTGAATTTTGCGAAAGCATCGGA
TATTGCAGAGATGATTAACGGTGAAGGTAACATTAGTATGCTTTCTGATCGTGGCAGCATGACGATTGATGAGCGTACTA
ACTCATTATTGATCCGTGAGTTGCCGGAGAATATTCAAGTAATTCGTGAAATCATTGAATCATTAGATATCCCAGTAAAA
CAAGTTCAGATTGAAGCACGTATCGTGACTATCAATGAAGGTAACATGGATGAACTTGGCGTTCGCTGGGGCTTCACATC
GATTAATGGTAGTCATACGGTTGGTGGATCGATCGAGAACAACCTTGCAACTATTGGTCTCTACGATGGCGGCGGTGGCG
ACGGCGATGGTGAAGACGGTGGCGGCAGTGTTGGTATTGATGACTTCCTGAACGTTAACCTTGCTGCAACCAGCCCTAAT
GCAACCAGTATTGCTTTCCAAGTTGCAAAACTAGGGTCTGATACTTTGTTGGATTTGGAGTTGTCTGCGTTGCAACAAGA
ATCAAAAGCAGAAATCATTTCTAGTCCGCGTTTAATTACTACCAATAAGAAGCCAGCTTATATTGAACAAGGTACTGAAA
TTCCTTACCTAGAATCCTCTTCCAGCGGTGCGACATCCGTAACGTTCAAAAAAGCGGTGCTGAGCTTAAAAGTGACACCG
CAGATCACGCCTGATAACCGTTTGGTGCTGGATTTGAGTGTTACGCAAGATAGACCGGGTCAAGTGGTAAAAACTGGTAC
CGGTGAGGCAGTTGCGATTGATACTCAGCGTATTGGCACTCAAGTATTGGTGAATAATGGTGAGACGGTTGTGCTTGGTG
GTATTTTCCAACACAGCATTACAAACTCCGTCGATAAGGTACCTTTATTAGGTGATTTACCGCTGTTAGGGGCACTTTTC
CGCCGAAGTTACGAAAATGTCGGTAAAAGTGAGTTGCTGATTTTTGTGACGCCTAAAGTGGTGATTCAGTAA

Domains

Predicted by InterproScan.

Bacterial type II and III secretion system protein (Secretin)

(423-581)

Bacterial type II/III secretion system short domain (Secretin_N)

(256-321)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Vibrio campbellii strain DS40M4

97.084

100

0.971
  pilQ Vibrio cholerae strain A1552

77.329

97.599

0.755
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

77.329

97.599

0.755
  pilQ Pseudomonas aeruginosa PAK

40.138

99.142

0.398
  pilQ Legionella pneumophila strain ERS1305867

41.273

94.34

0.389
  pilQ Legionella pneumophila strain Lp02

41.273

94.34

0.389
  pilQ Acinetobacter baumannii D1279779

36.833

100

0.379
  pilQ Acinetobacter baumannii strain A118

36.833

100

0.379
  comQ Acinetobacter baylyi ADP1

36.986

100

0.37