Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   Q5420_RS28050 Genome accession   NZ_CP130751
Coordinates   6034716..6035339 (-) Length   207 a.a.
NCBI ID   WP_003103268.1    Uniprot ID   G3XD51
Organism   Pseudomonas aeruginosa strain AU10241 isolate AU10241_L13_Control     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6029716..6040339
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  Q5420_RS28030 (Q5420_28030) aroB 6030311..6031417 (-) 1107 WP_003107839.1 3-dehydroquinate synthase -
  Q5420_RS28035 (Q5420_28035) aroK 6031467..6031985 (-) 519 WP_003095833.1 shikimate kinase AroK -
  Q5420_RS28040 (Q5420_28040) pilQ 6031997..6034141 (-) 2145 WP_003103267.1 type 4a pilus secretin PilQ Machinery gene
  Q5420_RS28045 (Q5420_28045) comL/pilP 6034195..6034719 (-) 525 WP_003095836.1 type 4a pilus biogenesis lipoprotein PilP Machinery gene
  Q5420_RS28050 (Q5420_28050) pilO 6034716..6035339 (-) 624 WP_003103268.1 type 4a pilus biogenesis protein PilO Machinery gene
  Q5420_RS28055 (Q5420_28055) pilN 6035336..6035932 (-) 597 WP_003095839.1 type 4a pilus biogenesis protein PilN Machinery gene
  Q5420_RS28060 (Q5420_28060) pilM 6035932..6036996 (-) 1065 WP_003110888.1 type IV pilus assembly protein PilM Machinery gene
  Q5420_RS28065 (Q5420_28065) - 6037181..6039652 (+) 2472 WP_061189531.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 207 a.a.        Molecular weight: 22819.26 Da        Isoelectric Point: 4.7787

>NTDB_id=787782 Q5420_RS28050 WP_003103268.1 6034716..6035339(-) (pilO) [Pseudomonas aeruginosa strain AU10241 isolate AU10241_L13_Control]
MSLASSLESLRKIDINDLDLNNIGSWPAAVKVIVCVLLTAAVLALGYNFHLSDMQAQLEQQAAEEETLKQQFSTKAFQAA
NLEAYKAQMKEMEESFGALLRQLPSDTEVPGLLEDITRTGLGSGLEFEEIKLLPEVAQQFYIELPIQISVVGGYHDLATF
VSGVSSLPRIVTLHDFEIKPVAPGSTSKLRMSILAKTYRYNDKGLKK

Nucleotide


Download         Length: 624 bp        

>NTDB_id=787782 Q5420_RS28050 WP_003103268.1 6034716..6035339(-) (pilO) [Pseudomonas aeruginosa strain AU10241 isolate AU10241_L13_Control]
ATGAGTCTGGCCAGTTCCCTGGAAAGTCTGCGCAAGATCGATATCAACGATCTCGACCTGAACAACATCGGTTCCTGGCC
GGCGGCGGTCAAGGTCATCGTCTGCGTGCTGCTGACCGCGGCGGTCCTGGCGCTGGGCTACAACTTCCATCTGAGTGACA
TGCAGGCTCAGCTCGAACAGCAGGCCGCGGAAGAGGAGACGCTCAAGCAGCAGTTCTCCACCAAGGCCTTCCAGGCCGCG
AACCTGGAAGCCTACAAGGCACAGATGAAGGAGATGGAAGAGTCCTTTGGCGCCTTGCTGCGGCAGTTGCCCAGCGACAC
CGAGGTACCCGGGCTGCTCGAGGACATCACTCGTACCGGCCTGGGCAGCGGCCTGGAGTTCGAGGAAATCAAGCTGCTTC
CCGAGGTTGCCCAGCAGTTCTACATCGAGCTGCCGATCCAGATCAGCGTGGTCGGCGGCTACCACGACTTGGCGACCTTC
GTCAGCGGCGTGTCCAGCCTGCCGCGGATCGTCACCCTGCATGACTTCGAGATCAAGCCGGTCGCGCCCGGCAGCACGTC
CAAGCTGCGCATGAGCATCCTGGCCAAGACCTATCGCTACAACGACAAGGGGCTGAAGAAATGA

Domains

Predicted by InterProScan.

Pilus assembly protein, PilO (PilO)

(58-202)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB G3XD51

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Legionella pneumophila strain ERS1305867

46.114

93.237

0.43
  pilO Acinetobacter baumannii D1279779

43.979

92.271

0.406
  comO/pilO Acinetobacter baylyi ADP1

43.455

92.271

0.401