Detailed information NTG0078573

Bioinformatically predicted

Overview

• Name: comGF
• Type: Machinery gene
• Locus tag: LLL8_RS11090
• Genome accession: NZ_AP025700
• Coordinates: 2245730..2246176 (-)
• Length: 148 a.a.
• NCBI ID: WP_029344525.1
• Uniprot ID: -
• Organism: Lactococcus lactis strain L8
• Function: dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2240730..2251176

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LLL8_RS11055 (LLL8_21660)	-	2240847..2241224 (-)	378	WP_033900715.1	pyridoxamine 5'-phosphate oxidase family protein	-
LLL8_RS11060 (LLL8_21670)	-	2241429..2242295 (+)	867	WP_251899925.1	RluA family pseudouridine synthase	-
LLL8_RS11065 (LLL8_21680)	-	2242333..2243142 (-)	810	WP_014570791.1	metal ABC transporter permease	-
LLL8_RS11070 (LLL8_21690)	-	2243135..2243872 (-)	738	WP_012898617.1	metal ABC transporter ATP-binding protein	-
LLL8_RS11075 (LLL8_21700)	-	2244049..2244891 (-)	843	WP_015427160.1	metal ABC transporter substrate-binding protein	-
LLL8_RS11080 (LLL8_21710)	-	2244888..2245325 (-)	438	WP_201248645.1	zinc-dependent MarR family transcriptional regulator	-
LLL8_RS11085 (LLL8_21720)	comGG	2245407..2245691 (-)	285	WP_025017139.1	competence type IV pilus minor pilin ComGG	Machinery gene
LLL8_RS11090 (LLL8_21730)	comGF	2245730..2246176 (-)	447	WP_029344525.1	competence type IV pilus minor pilin ComGF	Machinery gene
LLL8_RS11095 (LLL8_21740)	comGE	2246139..2246435 (-)	297	WP_010906316.1	competence type IV pilus minor pilin ComGE	Machinery gene
LLL8_RS11100 (LLL8_21750)	comGD	2246407..2246823 (-)	417	WP_032941843.1	competence type IV pilus minor pilin ComGD	Machinery gene
LLL8_RS11105 (LLL8_21760)	comGC	2246798..2247067 (-)	270	WP_021721869.1	competence type IV pilus major pilin ComGC	Machinery gene
LLL8_RS11110 (LLL8_21770)	-	2247121..2248368 (-)	1248	WP_251899926.1	Fic family protein	-
LLL8_RS11120 (LLL8_21780)	-	2249061..2249840 (-)	780	WP_350028262.1	peptidoglycan amidohydrolase family protein	-
LLL8_RS11125 (LLL8_21790)	-	2249840..2250127 (-)	288	WP_251899928.1	phage holin	-
LLL8_RS11130 (LLL8_21800)	-	2250140..2250367 (-)	228	WP_251899929.1	hemolysin XhlA family protein	-

Sequence

Protein

• Length: 148 a.a.
• Molecular weight: 17111.91 Da
• Isoelectric Point: 8.6780

>NTDB_id=78573 LLL8_RS11090 WP_029344525.1 2245730..2246176(-) (comGF) [Lactococcus lactis strain L8]
MERKLCDLNFKVKAFTLLECLVALLAISGSVLVISGLTKMLKEQVAISQSDSIKDWQIFCQQMRFELSGTKLDRVEQNFL
YVTKDKKLRFGFMSDDFRKTDDKGQGYQPMLYDIKAAKIQATKNLITIRIDFNQGGERTFIYQFPEDT

Nucleotide

• Length: 447 bp

>NTDB_id=78573 LLL8_RS11090 WP_029344525.1 2245730..2246176(-) (comGF) [Lactococcus lactis strain L8]
ATGGAAAGGAAATTATGCGACTTGAACTTCAAAGTTAAAGCATTTACCTTGTTGGAGTGTTTGGTGGCACTTCTTGCAAT
TTCTGGTTCAGTCCTTGTCATCTCAGGTTTAACAAAGATGTTGAAAGAACAAGTGGCGATTAGTCAAAGTGATAGCATAA
AAGATTGGCAAATTTTCTGTCAGCAAATGCGTTTTGAACTCTCAGGAACAAAATTAGATAGGGTAGAACAGAATTTTCTG
TATGTAACTAAAGATAAAAAACTAAGGTTTGGTTTTATGAGTGATGATTTCCGGAAGACTGATGACAAAGGTCAGGGTTA
TCAGCCAATGCTTTATGATATAAAAGCGGCTAAGATTCAAGCTACTAAAAATTTAATAACCATAAGAATTGATTTTAATC
AAGGAGGTGAAAGAACATTTATTTATCAATTTCCAGAAGATACGTAA

Domains

Predicted by InterProScan.

• Putative Competence protein ComGF (ComGF) (48-137)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comGF	Lactococcus lactis subsp. cremoris KW2	76.259	93.919	0.716
comGF	Streptococcus mutans UA140	48.227	95.27	0.459
comGF	Streptococcus mutans UA159	47.518	95.27	0.453
comGF/cglF	Streptococcus mitis SK321	43.066	92.568	0.399
comGF/cglF	Streptococcus pneumoniae Rx1	40.426	95.27	0.385
comGF/cglF	Streptococcus pneumoniae D39	40.426	95.27	0.385
comGF/cglF	Streptococcus pneumoniae R6	40.426	95.27	0.385
comGF/cglF	Streptococcus pneumoniae TIGR4	40.426	95.27	0.385
comGF/cglF	Streptococcus mitis NCTC 12261	41.606	92.568	0.385