Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   PSH58_RS24485 Genome accession   NZ_CP117434
Coordinates   5430288..5431988 (+) Length   566 a.a.
NCBI ID   WP_305385932.1    Uniprot ID   -
Organism   Pseudomonas hefeiensis strain FP53     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5425288..5436988
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PSH58_RS24465 (PSH58_24465) - 5425452..5428386 (+) 2935 Protein_4826 DUF748 domain-containing protein -
  PSH58_RS24470 (PSH58_24470) - 5428388..5428684 (+) 297 WP_305385929.1 DUF2845 domain-containing protein -
  PSH58_RS24475 (PSH58_24475) - 5429010..5429363 (-) 354 WP_305385930.1 BON domain-containing protein -
  PSH58_RS24480 (PSH58_24480) pilA 5429643..5430062 (-) 420 WP_305385931.1 pilin Machinery gene
  PSH58_RS24485 (PSH58_24485) pilB 5430288..5431988 (+) 1701 WP_305385932.1 type IV-A pilus assembly ATPase PilB Machinery gene
  PSH58_RS24490 (PSH58_24490) pilC 5431991..5433208 (+) 1218 WP_305385933.1 type II secretion system F family protein Machinery gene
  PSH58_RS24495 (PSH58_24495) - 5433210..5434081 (+) 872 Protein_4832 A24 family peptidase -
  PSH58_RS24500 (PSH58_24500) coaE 5434093..5434716 (+) 624 WP_305385934.1 dephospho-CoA kinase -
  PSH58_RS24505 (PSH58_24505) yacG 5434713..5434913 (+) 201 WP_305385935.1 DNA gyrase inhibitor YacG -
  PSH58_RS24510 (PSH58_24510) - 5435014..5435229 (-) 216 WP_305385936.1 hypothetical protein -
  PSH58_RS24515 (PSH58_24515) - 5435293..5435979 (-) 687 WP_305385937.1 energy-coupling factor ABC transporter permease -
  PSH58_RS24520 (PSH58_24520) - 5436124..5436750 (+) 627 WP_305385938.1 DUF1780 domain-containing protein -

Sequence

Protein


Download         Length: 566 a.a.        Molecular weight: 62748.74 Da        Isoelectric Point: 5.4194

>NTDB_id=785019 PSH58_RS24485 WP_305385932.1 5430288..5431988(+) (pilB) [Pseudomonas hefeiensis strain FP53]
MNDIALSGLAKQLVLAELITEQSAQQAYQQAQSSRMPLVSYLVQNKLVQSRQVAEIASEHFGVALLDLNSLDKETQPTGL
VSEKLVRQHHALPLWRRGNKLFVGISDPTNHQAINDIQFSTGLTTEAILVEDDKLSDAIEKFFESTSTGLEGMGDVDLDG
LNIESIDDHKQDSIAGQDTDDAPVVRFVNKMLLDAIKGGSSDLHFEPYEKIYRVRVRTDGILREVARPPIQLGTRIAARL
KVMASLDISERRKPQDGRLKMRLSQNKSIDFRVNTLPTLWGEKVVIRILDPSSAQMGIDALGYEPDQKELYMAALKQPQG
LILVTGPTGSGKTVSLYTGLNILNTVDINISTAEDPVEINMEGINQVNVNPRQGLDFAQALRSFLRQDPDVIMVGEIRDL
ETAEIAIKAAQTGHMVLSTLHTNSAAETLIRLHNMGIPGFNIATAVHLIIAQRLARKLCPHCKRAIEIPEETLFKEGFPR
ERIGSFTIYEPVGCEQCNNGYKGRVGVYEVVRNTPELQRLIMAEGNSLEIDLQMRKDGFNDLRASGLLKVMQGITSLEEI
NRVTKD

Nucleotide


Download         Length: 1701 bp        

>NTDB_id=785019 PSH58_RS24485 WP_305385932.1 5430288..5431988(+) (pilB) [Pseudomonas hefeiensis strain FP53]
ATGAATGACATCGCCTTAAGCGGTCTGGCCAAGCAATTGGTGCTGGCCGAACTGATCACCGAGCAAAGTGCGCAGCAAGC
GTACCAACAGGCCCAAAGCAGCCGCATGCCCCTGGTCAGTTACCTGGTGCAGAACAAACTGGTCCAGAGCCGCCAAGTGG
CGGAAATTGCCTCGGAGCATTTCGGCGTGGCCCTGCTGGACCTCAACAGCCTGGACAAGGAAACCCAACCCACCGGCCTT
GTCAGCGAAAAACTGGTGCGCCAGCATCACGCCCTGCCGCTGTGGCGGCGTGGCAACAAGCTGTTCGTGGGCATCTCCGA
CCCCACCAATCACCAGGCCATCAATGACATTCAGTTCAGCACCGGCCTGACCACTGAAGCCATCCTGGTGGAGGACGACA
AGCTCAGCGATGCCATCGAAAAGTTTTTCGAATCCACCAGCACCGGCCTGGAAGGCATGGGCGATGTCGACCTCGACGGC
CTGAACATCGAGTCGATCGATGATCACAAGCAGGACTCCATCGCTGGCCAGGACACCGACGACGCGCCGGTGGTGCGGTT
CGTCAACAAGATGCTGCTGGACGCAATCAAGGGCGGCTCCTCCGACCTGCACTTTGAACCCTATGAAAAAATCTACCGGG
TGCGGGTGCGTACCGACGGCATACTGCGGGAAGTGGCCCGCCCGCCGATCCAGCTGGGCACCCGCATCGCCGCGCGCCTG
AAGGTCATGGCCAGCCTCGATATTTCCGAGCGGCGCAAACCCCAGGACGGGCGCCTGAAAATGCGCCTGTCGCAAAACAA
ATCCATCGATTTCCGGGTCAACACACTGCCGACGCTCTGGGGTGAAAAAGTGGTGATCCGGATCCTCGACCCCTCCAGCG
CGCAGATGGGTATCGATGCCCTGGGCTACGAGCCGGACCAGAAAGAGCTGTACATGGCGGCGCTCAAACAACCACAAGGG
TTGATCCTGGTGACCGGGCCCACCGGCTCTGGCAAAACCGTGTCGCTCTATACCGGGCTGAATATCCTCAACACCGTGGA
CATCAATATTTCCACCGCTGAGGACCCCGTGGAGATCAACATGGAAGGCATTAACCAGGTCAATGTGAATCCACGCCAGG
GCCTGGATTTTGCCCAGGCCCTGCGCTCGTTTCTGCGCCAGGACCCGGACGTGATCATGGTGGGCGAGATCCGCGACCTG
GAAACGGCCGAAATCGCCATCAAGGCCGCCCAGACCGGACATATGGTGCTCTCCACCCTGCACACCAACAGCGCCGCCGA
AACCCTGATCCGTCTGCACAACATGGGCATTCCCGGTTTCAACATCGCCACCGCGGTCCACCTGATCATCGCCCAGCGAC
TGGCGCGCAAACTGTGCCCCCATTGCAAAAGGGCCATCGAGATTCCCGAGGAAACCCTGTTCAAGGAAGGCTTCCCCCGG
GAACGCATCGGCTCGTTCACGATCTATGAGCCGGTCGGTTGCGAACAATGCAACAACGGCTACAAGGGGCGCGTAGGGGT
TTACGAAGTGGTCAGGAATACGCCCGAGCTGCAACGGCTGATCATGGCTGAGGGCAATTCGCTGGAAATCGACCTGCAAA
TGCGCAAGGACGGCTTCAATGACTTGCGCGCGTCGGGACTGCTCAAAGTGATGCAAGGCATCACCAGTCTCGAAGAAATC
AACCGGGTCACCAAGGATTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(190-457)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-146)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

57.597

100

0.576
  pilB Acinetobacter baumannii D1279779

56.306

99.47

0.56
  pilB Legionella pneumophila strain ERS1305867

55.458

100

0.557
  pilB Vibrio cholerae strain A1552

50.619

99.823

0.505
  pilB Vibrio campbellii strain DS40M4

48.246

100

0.486
  pilF Neisseria gonorrhoeae MS11

48.313

99.47

0.481
  pilB Vibrio parahaemolyticus RIMD 2210633

49.453

96.82

0.479
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

36.833

99.293

0.366
  pilB/pilB1 Synechocystis sp. PCC 6803

39.382

91.519

0.36