Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   PJU73_RS06895 Genome accession   NZ_CP116766
Coordinates   1502047..1503720 (+) Length   557 a.a.
NCBI ID   WP_237091277.1    Uniprot ID   -
Organism   Neisseria lisongii strain ZJ106     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1497047..1508720
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PJU73_RS06870 (PJU73_06870) - 1497944..1498507 (-) 564 WP_237091281.1 hypothetical protein -
  PJU73_RS06880 (PJU73_06880) - 1499143..1499400 (+) 258 WP_237091280.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
  PJU73_RS06885 (PJU73_06885) - 1499400..1499825 (+) 426 WP_237091279.1 type II toxin-antitoxin system VapC family toxin -
  PJU73_RS06890 (PJU73_06890) - 1499935..1501680 (-) 1746 WP_237091278.1 potassium/proton antiporter -
  PJU73_RS06895 (PJU73_06895) pilF 1502047..1503720 (+) 1674 WP_237091277.1 type IV-A pilus assembly ATPase PilB Machinery gene
  PJU73_RS06900 (PJU73_06900) pilG 1503778..1505010 (+) 1233 WP_237091276.1 type II secretion system F family protein Machinery gene
  PJU73_RS06905 (PJU73_06905) pilD 1505081..1505956 (+) 876 WP_237091275.1 A24 family peptidase Machinery gene
  PJU73_RS06910 (PJU73_06910) coaE 1505966..1506562 (+) 597 WP_237091274.1 dephospho-CoA kinase -
  PJU73_RS06915 (PJU73_06915) - 1506962..1507906 (+) 945 WP_272607677.1 IS110 family transposase -
  PJU73_RS06920 (PJU73_06920) yacG 1508259..1508456 (+) 198 WP_237092024.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 557 a.a.        Molecular weight: 61534.72 Da        Isoelectric Point: 6.0884

>NTDB_id=780001 PJU73_RS06895 WP_237091277.1 1502047..1503720(+) (pilF) [Neisseria lisongii strain ZJ106]
MSSALLRVLTQHHILTPAQAEHYHQAAAEHQAVLPKLFADGIISPQDLAQRLAQIFHYPLLDLRDYDRSLLVKEVLSDEQ
MVQNRCVPLFRRGETVCLAVSDPTQIRHLQKIAFAAGVAIDLVVVADDQLSALLDWINQHSAALLQEIGDSPETGTQTLY
IDNEEAEDGPIPRFIHKILSNALAAGASDIHFEFYEHSARIRFRIDGTLREVVQPPLAVRHQLASCIKVMARLDIAEKRV
PQDGRIRIALQKNAAAIDFRVSTLPTVFGEKVVMRILNTDIGRLNFAELGLEPFQQTMLADAVRRPYGMVLVTGPTGSGK
TLTLYACLKLLNTEGVNIATAEDPAEIHLAGINQVNVNDRQGLTFAAALRAFLRQDPDIIMVGEIRDLETADIAIKAAQT
GHMVFSTLHTNNAPATLSRLLNMGVEPFNIAGSVSLIVAQRLVRRLCPDCKQPTERPPEAVLRSLGFTDEDLQQNWQPYR
PVGCDHCRGKGFKGRIGIFELMPVSETVQQIILNNGTETDMLNQAAREGMTDLRRAGLLKVMQGLTSLEEVCACTND

Nucleotide


Download         Length: 1674 bp        

>NTDB_id=780001 PJU73_RS06895 WP_237091277.1 1502047..1503720(+) (pilF) [Neisseria lisongii strain ZJ106]
ATGAGCAGCGCCCTTCTGCGTGTCCTCACGCAACACCATATCCTCACACCGGCACAGGCCGAGCATTATCATCAGGCGGC
GGCGGAACATCAGGCCGTTTTGCCGAAGCTATTTGCCGATGGCATCATTTCGCCGCAGGATTTGGCGCAACGGCTGGCGC
AGATTTTCCATTATCCGCTGCTGGATTTGCGGGATTACGACCGCAGTCTGTTGGTCAAAGAAGTCTTGAGCGACGAACAA
ATGGTGCAAAACCGCTGCGTGCCGCTGTTTCGGCGGGGCGAAACCGTGTGTCTGGCGGTTTCCGACCCGACTCAAATCCG
CCATTTGCAGAAAATCGCCTTTGCCGCCGGCGTTGCTATTGATTTGGTGGTGGTAGCCGACGACCAGCTCTCGGCGCTGT
TGGACTGGATCAACCAACATTCCGCCGCCCTGCTGCAGGAAATCGGCGACAGCCCCGAAACCGGCACGCAAACGCTCTAC
ATCGACAACGAAGAAGCCGAAGACGGCCCGATTCCCCGCTTTATCCACAAAATCCTCTCCAATGCGCTGGCCGCCGGCGC
ATCCGACATTCATTTCGAATTTTACGAACACAGCGCCCGAATCCGTTTCCGCATTGACGGCACATTGCGCGAAGTAGTGC
AGCCGCCGCTTGCCGTGCGACACCAGCTCGCCTCGTGCATCAAAGTGATGGCACGTTTGGACATTGCCGAAAAACGGGTG
CCGCAAGACGGGCGTATCCGCATTGCCCTGCAGAAAAACGCAGCGGCGATTGACTTTCGGGTCAGTACTTTGCCGACCGT
GTTCGGCGAAAAAGTGGTGATGCGGATTTTGAACACCGACATCGGCCGTCTGAATTTTGCCGAACTCGGCTTAGAGCCGT
TCCAACAGACCATGCTCGCCGATGCCGTACGCCGTCCCTACGGCATGGTTTTGGTTACCGGCCCGACCGGTTCGGGCAAA
ACGCTCACACTCTATGCCTGTCTGAAGCTGCTCAACACCGAAGGTGTCAATATCGCCACCGCCGAAGATCCGGCGGAAAT
CCATCTGGCGGGCATCAATCAGGTCAATGTCAATGACCGACAAGGGCTGACGTTTGCCGCCGCCCTGCGGGCATTTCTGC
GGCAAGACCCCGATATCATTATGGTCGGCGAAATCCGGGATTTGGAAACCGCCGACATCGCCATCAAAGCGGCGCAAACC
GGCCACATGGTTTTTTCCACGCTGCACACCAACAATGCCCCCGCCACCCTCTCCCGCCTGCTGAATATGGGTGTCGAACC
GTTCAACATCGCCGGTTCGGTCAGCCTGATTGTGGCGCAGCGGCTGGTGCGGCGGCTGTGTCCCGACTGCAAACAGCCCA
CCGAACGCCCGCCTGAAGCCGTCTTGCGCTCACTCGGTTTCACCGATGAAGATTTACAGCAAAACTGGCAACCCTACCGC
CCTGTCGGTTGCGATCATTGTCGGGGCAAAGGTTTCAAAGGACGGATCGGCATTTTCGAACTCATGCCCGTCAGCGAAAC
CGTGCAGCAGATTATTCTCAACAACGGCACCGAAACCGATATGCTCAATCAGGCGGCTCGGGAAGGCATGACCGACCTGC
GGCGTGCCGGGTTGCTCAAAGTGATGCAGGGTCTGACTTCCTTAGAAGAAGTCTGCGCCTGCACCAATGATTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(177-445)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(37-141)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Neisseria gonorrhoeae MS11

68.1

100

0.682
  pilB Acinetobacter baylyi ADP1

47.173

100

0.479
  pilB Acinetobacter baumannii D1279779

47.237

100

0.476
  pilB Legionella pneumophila strain ERS1305867

46.359

100

0.469
  pilB Vibrio parahaemolyticus RIMD 2210633

42.982

100

0.44
  pilB Vibrio campbellii strain DS40M4

42.531

100

0.434
  pilB Vibrio cholerae strain A1552

42.43

100

0.433
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.255

94.434

0.39
  pilF Thermus thermophilus HB27

39.434

95.153

0.375