Detailed information NTG0770646

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: PCP26_RS03085
• Genome accession: NZ_CP115260
• Coordinates: 668639..669787 (+)
• Length: 382 a.a.
• NCBI ID: WP_003110016.1
• Uniprot ID: A0A0H2ZL88
• Organism: Pseudomonas aeruginosa strain F026
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 663639..674787

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PCP26_RS03060 (PCP26_03060)	-	664790..665086 (-)	297	WP_031627661.1	DUF167 domain-containing protein	-
PCP26_RS03065 (PCP26_03065)	-	665083..665676 (-)	594	WP_003084544.1	YggT family protein	-
PCP26_RS03070 (PCP26_03070)	proC	665687..666508 (-)	822	WP_003084546.1	pyrroline-5-carboxylate reductase	-
PCP26_RS03075 (PCP26_03075)	-	666520..667212 (-)	693	WP_003084549.1	YggS family pyridoxal phosphate-dependent enzyme	-
PCP26_RS03080 (PCP26_03080)	pilT	667427..668461 (+)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
PCP26_RS03085 (PCP26_03085)	pilU	668639..669787 (+)	1149	WP_003110016.1	type IV pilus ATPase PilU	Machinery gene
PCP26_RS03090 (PCP26_03090)	czcD	669795..670694 (-)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
PCP26_RS03095 (PCP26_03095)	-	670848..671252 (+)	405	WP_003084560.1	NINE protein	-
PCP26_RS03100 (PCP26_03100)	-	671495..672868 (+)	1374	WP_003100955.1	pyridoxal-phosphate dependent enzyme	-
PCP26_RS03105 (PCP26_03105)	-	672865..674049 (+)	1185	WP_003110017.1	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42559.83 Da
• Isoelectric Point: 6.7792

>NTDB_id=770646 PCP26_RS03085 WP_003110016.1 668639..669787(+) (pilU) [Pseudomonas aeruginosa strain F026]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSNLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=770646 PCP26_RS03085 WP_003110016.1 668639..669787(+) (pilU) [Pseudomonas aeruginosa strain F026]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCTGATCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAACCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: A0A0H2ZL88

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.22	100	0.882
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382