Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   PCP14_RS04850 Genome accession   NZ_CP115251
Coordinates   1023524..1023976 (-) Length   150 a.a.
NCBI ID   WP_024947914.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain F035     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1018524..1028976
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PCP14_RS04830 (PCP14_04820) ampD 1019230..1019796 (-) 567 WP_003112844.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  PCP14_RS04835 (PCP14_04825) - 1019947..1022202 (-) 2256 WP_016264271.1 DUF1631 domain-containing protein -
  PCP14_RS04840 (PCP14_04830) nadC 1022488..1023336 (+) 849 WP_024947913.1 carboxylating nicotinate-nucleotide diphosphorylase -
  PCP14_RS04850 (PCP14_04840) pilA 1023524..1023976 (-) 453 WP_024947914.1 pilin Machinery gene
  PCP14_RS04855 (PCP14_04845) pilB 1024205..1025905 (+) 1701 WP_009875880.1 type IV-A pilus assembly ATPase PilB Machinery gene
  PCP14_RS04860 (PCP14_04850) pilC 1025909..1027129 (+) 1221 WP_016253888.1 type 4a pilus biogenesis protein PilC Machinery gene
  PCP14_RS04865 (PCP14_04855) pilD 1027133..1028005 (+) 873 WP_003125204.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  PCP14_RS04870 (PCP14_04860) coaE 1028002..1028613 (+) 612 WP_003094654.1 dephospho-CoA kinase -
  PCP14_RS04875 (PCP14_04865) yacG 1028610..1028810 (+) 201 WP_017148551.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 150 a.a.        Molecular weight: 15622.02 Da        Isoelectric Point: 8.4827

>NTDB_id=770338 PCP14_RS04850 WP_024947914.1 1023524..1023976(-) (pilA) [Pseudomonas aeruginosa strain F035]
MKAQKGFTLIELMIVVAIIGILAAIAIPQYQNYVARSEGASALASVNPLKTTVEEALSRGWSVKSGTGTEDATKKEVPLG
VAADANKLGTIALKPDPADGTADITLTFTMGGAGPKNKGKIITLTRTAADGLWKCTSDQDEQFIPKGCSK

Nucleotide


Download         Length: 453 bp        

>NTDB_id=770338 PCP14_RS04850 WP_024947914.1 1023524..1023976(-) (pilA) [Pseudomonas aeruginosa strain F035]
ATGAAAGCTCAAAAAGGCTTTACCTTGATCGAACTGATGATCGTGGTTGCGATCATCGGTATCTTGGCTGCAATTGCCAT
TCCTCAGTATCAGAATTATGTAGCTCGTTCGGAAGGCGCATCTGCTCTTGCTTCGGTCAATCCGTTGAAGACTACCGTTG
AAGAGGCGCTTTCTCGTGGTTGGAGCGTGAAGAGCGGTACAGGTACAGAGGACGCTACTAAGAAAGAGGTTCCTCTGGGG
GTGGCGGCAGATGCTAACAAACTGGGTACTATCGCACTCAAACCCGATCCTGCTGATGGTACTGCAGATATTACTTTGAC
TTTCACTATGGGCGGTGCAGGACCGAAGAATAAAGGGAAAATTATTACCCTGACTCGTACTGCAGCTGATGGTCTCTGGA
AGTGCACCAGTGATCAGGATGAGCAGTTTATTCCGAAAGGTTGCTCTAAGTAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(35-148)

Prokaryotic N-terminal methylation motif (N_methyl)

(1-26)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Pseudomonas aeruginosa PAK

100

100

1
  pilA/pilAI Pseudomonas stutzeri DSM 10701

47.682

100

0.48
  pilA Acinetobacter baumannii strain A118

45.333

100

0.453
  pilA/pilAII Pseudomonas stutzeri DSM 10701

46.575

97.333

0.453
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

42.675

100

0.447
  pilA Vibrio cholerae C6706

42.675

100

0.447
  pilA Vibrio cholerae strain A1552

42.675

100

0.447
  pilA Vibrio parahaemolyticus RIMD 2210633

48.507

89.333

0.433
  comP Acinetobacter baylyi ADP1

40.789

100

0.413
  pilA2 Legionella pneumophila strain ERS1305867

39.865

98.667

0.393
  pilA/pilA1 Eikenella corrodens VA1

39.456

98

0.387
  pilA2 Legionella pneumophila str. Paris

39.189

98.667

0.387