Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   O9K61_RS11995 Genome accession   NZ_CP115185
Coordinates   2472284..2473354 (-) Length   356 a.a.
NCBI ID   WP_012117985.1    Uniprot ID   A0A411A7V3
Organism   Bacillus velezensis strain 26.3     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2467284..2478354
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  O9K61_RS11940 (O9K61_11940) sipW 2467351..2467935 (-) 585 WP_015240205.1 signal peptidase I SipW -
  O9K61_RS11945 (O9K61_11945) tapA 2467907..2468578 (-) 672 WP_020954230.1 amyloid fiber anchoring/assembly protein TapA -
  O9K61_RS11950 (O9K61_11950) - 2468837..2469166 (+) 330 WP_020954231.1 DUF3889 domain-containing protein -
  O9K61_RS11955 (O9K61_11955) - 2469206..2469385 (-) 180 WP_003153093.1 YqzE family protein -
  O9K61_RS11960 (O9K61_11960) comGG 2469442..2469819 (-) 378 WP_032866434.1 competence type IV pilus minor pilin ComGG Machinery gene
  O9K61_RS11965 (O9K61_11965) comGF 2469820..2469987 (-) 168 WP_235183824.1 ComGF family competence protein Machinery gene
  O9K61_RS11970 (O9K61_11970) - 2469984..2470178 (-) 195 WP_225917419.1 hypothetical protein -
  O9K61_RS11975 (O9K61_11975) comGE 2470222..2470536 (-) 315 WP_007408323.1 competence type IV pilus minor pilin ComGE -
  O9K61_RS11980 (O9K61_11980) comGD 2470520..2470957 (-) 438 WP_007408322.1 competence type IV pilus minor pilin ComGD Machinery gene
  O9K61_RS11985 (O9K61_11985) comGC 2470947..2471255 (-) 309 WP_003153087.1 competence type IV pilus major pilin ComGC Machinery gene
  O9K61_RS11990 (O9K61_11990) comGB 2471260..2472297 (-) 1038 WP_032866436.1 competence type IV pilus assembly protein ComGB Machinery gene
  O9K61_RS11995 (O9K61_11995) comGA 2472284..2473354 (-) 1071 WP_012117985.1 competence type IV pilus ATPase ComGA Machinery gene
  O9K61_RS12000 (O9K61_12000) - 2473546..2474496 (-) 951 WP_015417820.1 magnesium transporter CorA family protein -
  O9K61_RS12005 (O9K61_12005) - 2474642..2475943 (+) 1302 WP_032866438.1 hemolysin family protein -
  O9K61_RS12010 (O9K61_12010) - 2475981..2476811 (-) 831 WP_015240214.1 STAS domain-containing protein -
  O9K61_RS12020 (O9K61_12020) - 2477018..2477398 (-) 381 WP_003153079.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  O9K61_RS12025 (O9K61_12025) - 2477635..2477880 (+) 246 WP_003153078.1 DUF2626 domain-containing protein -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 40557.86 Da        Isoelectric Point: 9.4784

>NTDB_id=768761 O9K61_RS11995 WP_012117985.1 2472284..2473354(-) (comGA) [Bacillus velezensis strain 26.3]
MKNIETYSRNIINEAYTARASDIHIVPRERDAFIHFRIGHALVKKRVLKKEECVRLISHFKFLSAMDIGERRKPQNGSLS
LPLPTETVHLRMSTLPTMNDESLVIRLLPKRQIPPLDKLSLFRGAGAALLSFLKHSHGLLLFTGPTGSGKTTTLYSLVNY
AKRHFNRRIVTLEDPVETRDEDVLQVQVNEKAGVTYSAGLKAILRHDPDMIILGEIRDAETAEIAVRAAMTGHLVLSSLH
TRDAKGAIYRLLEFGVNMTEIEQTVIAIAAQRLVDLTCPFCKEENCSVYCRMYRQTRRAGIYELLYGKNLTQCFQEAKGE
HANFQYQTLRRLIRKGIALGYVTTDNYDRWVYHEAD

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=768761 O9K61_RS11995 WP_012117985.1 2472284..2473354(-) (comGA) [Bacillus velezensis strain 26.3]
GTGAAAAATATAGAAACATACAGCAGGAACATCATAAACGAGGCCTATACGGCAAGGGCTTCAGATATACACATTGTGCC
GAGAGAAAGGGATGCTTTCATTCATTTTCGCATCGGCCATGCTCTTGTGAAAAAAAGAGTCTTAAAAAAAGAGGAATGCG
TAAGGCTAATTTCTCATTTTAAGTTTTTATCGGCCATGGATATAGGAGAAAGACGTAAGCCGCAAAACGGGTCATTGTCT
CTGCCGCTTCCGACGGAAACCGTTCATTTGAGAATGTCAACCCTGCCGACAATGAATGATGAAAGTCTTGTCATCAGGCT
TTTGCCTAAGCGGCAGATTCCCCCTCTGGATAAACTGTCCTTATTTCGGGGGGCGGGCGCCGCACTATTGTCCTTTCTGA
AGCATTCGCACGGCCTGCTTCTGTTTACCGGCCCCACCGGATCTGGAAAAACGACCACTCTTTACTCGCTTGTCAACTAT
GCAAAAAGGCATTTCAACCGGCGTATTGTCACGCTTGAAGACCCCGTAGAAACAAGAGATGAGGATGTGTTGCAAGTTCA
GGTGAATGAAAAAGCCGGAGTCACATATTCCGCCGGTTTAAAAGCGATTTTAAGACATGATCCCGATATGATCATCTTAG
GGGAAATCAGGGATGCCGAGACGGCTGAAATCGCAGTCCGCGCGGCCATGACGGGACACCTCGTTCTTTCAAGCCTGCAT
ACAAGAGATGCTAAGGGCGCCATATACAGGCTGCTGGAGTTCGGCGTGAACATGACAGAAATTGAACAAACCGTCATTGC
CATTGCCGCTCAGCGTCTGGTTGATTTAACATGTCCGTTCTGCAAAGAGGAAAACTGTTCCGTGTACTGCCGGATGTACA
GGCAGACACGCCGTGCGGGCATTTATGAACTGCTCTACGGGAAAAACCTCACCCAATGCTTTCAGGAAGCAAAGGGAGAG
CACGCCAATTTTCAATATCAGACGCTCCGCAGGCTGATCAGAAAAGGAATCGCGCTCGGCTATGTCACGACCGACAATTA
CGATCGGTGGGTGTATCATGAAGCGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A411A7V3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

82.303

100

0.823
  pilB Glaesserella parasuis strain SC1401

38.793

97.753

0.379
  pilF Thermus thermophilus HB27

37.892

98.596

0.374