Detailed information NTG0767993

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: OZ430_RS01490
• Genome accession: NZ_CP115039
• Coordinates: 314102..315247 (+)
• Length: 381 a.a.
• NCBI ID: WP_015275295.1
• Uniprot ID: A0A2N8SGR5
• Organism: Stutzerimonas kunmingensis strain LP1
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 309102..320247

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
OZ430_RS01470 (OZ430_01470)	-	310777..311367 (-)	591	WP_014818744.1	YggT family protein	-
OZ430_RS01475 (OZ430_01475)	proC	311418..312242 (-)	825	WP_125875977.1	pyrroline-5-carboxylate reductase	-
OZ430_RS01480 (OZ430_01480)	-	312253..312942 (-)	690	WP_102832307.1	YggS family pyridoxal phosphate-dependent enzyme	-
OZ430_RS01485 (OZ430_01485)	pilT	313007..314041 (+)	1035	WP_003303153.1	type IV pilus twitching motility protein PilT	Machinery gene
OZ430_RS01490 (OZ430_01490)	pilU	314102..315247 (+)	1146	WP_015275295.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
OZ430_RS01495 (OZ430_01495)	-	315244..316080 (-)	837	WP_312397457.1	TIGR03915 family putative DNA repair protein	-
OZ430_RS01500 (OZ430_01500)	-	316080..317291 (-)	1212	WP_312915156.1	putative DNA modification/repair radical SAM protein	-
OZ430_RS01505 (OZ430_01505)	-	317425..317820 (+)	396	WP_003282737.1	TM2 domain-containing protein	-
OZ430_RS01510 (OZ430_01510)	-	317898..319172 (-)	1275	WP_312914923.1	dihydroorotase	-
OZ430_RS01515 (OZ430_01515)	-	319172..320188 (-)	1017	WP_312914922.1	aspartate carbamoyltransferase catalytic subunit	-

Sequence

Protein

• Length: 381 a.a.
• Molecular weight: 42430.68 Da
• Isoelectric Point: 6.7256

>NTDB_id=767993 OZ430_RS01490 WP_015275295.1 314102..315247(+) (pilU) [Stutzerimonas kunmingensis strain LP1]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGKIMPVSKTAMSPEMTRETVHGVMNEQQRREFTENHECNFAISARGIGR
FRVSAFYQRNLAGMVLRRIETNIPTIEELKLPDVLKKLSMTKRGLVLFVGATGTGKSTSLASMIGYRNKNSSGHIISIED
PIEFIHQHQSCIVTQREVGIDTSSFEVALKNTLRQAPDVILIGEIRTRETMDYAVAFAETGHLCLATLHANNANQALDRI
INFFPPDRHNQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLINTPLAADLIRKGEVHELKSLMKRSTELGMQTFDQA
LYNLYVQGEITYEDALLHADSANDLRLLIKLGSETDGEHLTSVSQGLSLEVSDDDPGRSFR

Nucleotide

• Length: 1146 bp

>NTDB_id=767993 OZ430_RS01490 WP_015275295.1 314102..315247(+) (pilU) [Stutzerimonas kunmingensis strain LP1]
ATGGAATTCGAGAAACTTCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGATCTGTTCATCACGGCAGGCGTGCCGCCTTC
GATGAAGGTCAACGGAAAGATCATGCCGGTCAGCAAGACGGCGATGTCGCCGGAAATGACCCGCGAGACCGTGCACGGAG
TTATGAACGAGCAGCAGCGCCGCGAGTTCACGGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGTATCGGCCGT
TTCCGGGTCAGTGCGTTCTACCAGCGCAATCTCGCCGGCATGGTGCTGCGGCGTATCGAAACCAACATTCCGACCATCGA
AGAACTCAAGCTGCCGGACGTCCTCAAGAAGCTGTCGATGACCAAGCGCGGGCTGGTGCTGTTCGTCGGGGCCACCGGTA
CCGGCAAGTCGACCTCATTGGCTTCGATGATCGGCTACCGCAACAAGAATTCCAGCGGGCACATCATCTCCATCGAAGAC
CCGATCGAATTCATCCACCAGCATCAGAGCTGCATCGTTACCCAGCGCGAGGTCGGTATCGACACCAGCTCGTTCGAAGT
GGCGCTGAAGAACACCTTGCGCCAGGCGCCGGACGTGATCCTCATCGGCGAGATCCGTACCCGTGAAACCATGGACTACG
CCGTGGCCTTCGCCGAAACCGGTCACCTGTGCCTGGCGACCCTGCACGCCAACAACGCCAACCAGGCGCTGGACCGCATA
ATCAACTTCTTCCCGCCCGACCGTCACAACCAGGTGTGGATGGATCTGTCGCTCAACCTCAAGGCCATCGTCGCCCAGCA
GCTGGTGCCGACGCCTGACGGCAAGGGGCGTCGCGCGGTGATCGAGGTGCTGATCAATACGCCGCTGGCGGCCGACCTGA
TCCGCAAGGGCGAGGTGCATGAGCTCAAGTCGCTGATGAAGCGCTCCACCGAGCTGGGCATGCAGACCTTCGACCAGGCG
CTGTACAACCTCTATGTGCAGGGTGAAATCACCTACGAAGATGCGTTGCTGCACGCCGATTCGGCCAACGATTTGCGTCT
GCTGATCAAGCTGGGATCGGAAACCGATGGCGAGCATCTGACCAGCGTGTCCCAGGGCCTGAGCCTTGAGGTCAGTGACG
ACGATCCAGGCCGCAGCTTCCGCTAG

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (113-270)

3D structure

• AlphaFold DB: A0A2N8SGR5

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	95.013	100	0.95
pilU	Acinetobacter baylyi ADP1	66.106	93.701	0.619
pilU	Vibrio cholerae strain A1552	59.714	91.864	0.549
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.658	88.976	0.388