Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   OZ660_RS14975 Genome accession   NZ_CP113972
Coordinates   3299904..3301016 (-) Length   370 a.a.
NCBI ID   WP_202262273.1    Uniprot ID   -
Organism   Alteromonas sp. 009811495     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3294904..3306016
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OZ660_RS14945 (OZ660_14955) - 3294982..3295824 (-) 843 WP_202262268.1 mechanosensitive ion channel domain-containing protein -
  OZ660_RS14950 (OZ660_14960) - 3296668..3297396 (+) 729 WP_274741451.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  OZ660_RS14955 (OZ660_14965) gshB 3297545..3298498 (+) 954 WP_274741452.1 glutathione synthase -
  OZ660_RS14960 (OZ660_14970) - 3298548..3299114 (+) 567 WP_159626764.1 YqgE/AlgH family protein -
  OZ660_RS14965 (OZ660_14975) ruvX 3299107..3299538 (+) 432 WP_274741453.1 Holliday junction resolvase RuvX -
  OZ660_RS14970 (OZ660_14980) - 3299545..3299874 (-) 330 WP_274741454.1 DUF2007 domain-containing protein -
  OZ660_RS14975 (OZ660_14985) pilU 3299904..3301016 (-) 1113 WP_202262273.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  OZ660_RS14980 (OZ660_14990) pilT 3301026..3302069 (-) 1044 WP_159626757.1 type IV pilus twitching motility protein PilT Machinery gene
  OZ660_RS14985 (OZ660_14995) - 3302097..3302795 (+) 699 WP_274741455.1 YggS family pyridoxal phosphate-dependent enzyme -
  OZ660_RS14990 (OZ660_15000) proC 3302861..3303682 (+) 822 WP_274741456.1 pyrroline-5-carboxylate reductase -
  OZ660_RS14995 (OZ660_15005) - 3303704..3304243 (+) 540 WP_274741457.1 YggT family protein -
  OZ660_RS15000 (OZ660_15010) - 3304320..3304718 (+) 399 WP_274743465.1 DUF4426 domain-containing protein -

Sequence

Protein


Download         Length: 370 a.a.        Molecular weight: 40752.69 Da        Isoelectric Point: 6.0491

>NTDB_id=763868 OZ660_RS14975 WP_202262273.1 3299904..3301016(-) (pilU) [Alteromonas sp. 009811495]
MLDTLLQKMVDSNASDLFITAGFPVSAKINGKLTPITEGVSSEEASLALVHEAMSEKQRDEFHTSKECNFAIVREGIGRF
RCSAFWQRDQAGMVVRRIVTQIPKADDLGLPTVLKDIIMAKRGLVLFVGGTGTGKSTSLAALIGHRNENSHGHILTIEDP
IEFVHEHKNCVITQREVGIDTQSFDDALKSSLRQAPDVILIGEIRSMETMEYAMSFADTGHLCVATLHANNANQAIERIM
HLAPKDQHDKLRFDLSLNIRAIVAQQLVPTPDGKGRVAAIEILLNSPLVRDLIQRNEIGALKEAMKKGKEQGMQSFDMAL
YDLYKAGKIDMDQALHHADSPNDLRLMIKLDTKDGSSLGTLSNVSIDMDD

Nucleotide


Download         Length: 1113 bp        

>NTDB_id=763868 OZ660_RS14975 WP_202262273.1 3299904..3301016(-) (pilU) [Alteromonas sp. 009811495]
ATGCTAGATACCTTATTACAAAAAATGGTCGACTCGAACGCGTCCGATTTATTTATCACCGCAGGTTTTCCTGTCAGTGC
AAAAATTAACGGCAAGCTAACGCCAATTACGGAAGGTGTGTCGTCTGAAGAGGCCTCATTAGCATTGGTTCATGAAGCCA
TGAGTGAAAAACAGCGCGACGAATTTCACACGTCGAAAGAGTGTAACTTCGCTATTGTTCGAGAAGGTATTGGCCGTTTC
CGCTGCTCTGCTTTTTGGCAGCGTGATCAGGCGGGCATGGTGGTCCGTCGCATTGTGACTCAGATCCCAAAAGCAGACGA
TTTAGGTCTGCCAACTGTCCTAAAAGACATCATTATGGCCAAGCGGGGCTTGGTGCTTTTCGTGGGGGGCACGGGTACAG
GTAAATCAACGTCACTCGCGGCGCTCATTGGCCATAGAAATGAAAACTCTCACGGTCATATATTAACGATTGAAGACCCG
ATTGAATTTGTACACGAGCACAAAAATTGTGTTATCACTCAGCGAGAAGTAGGCATAGATACGCAGTCATTTGATGACGC
GCTTAAAAGCTCATTACGTCAAGCTCCAGATGTCATCCTCATTGGTGAGATTCGCTCAATGGAGACTATGGAGTACGCCA
TGTCTTTTGCTGATACTGGACACTTGTGTGTGGCAACACTTCACGCGAATAATGCCAATCAGGCTATCGAGCGCATTATG
CACCTTGCGCCAAAAGATCAGCACGACAAACTTCGTTTTGACTTAAGTCTGAATATTCGAGCAATTGTAGCGCAGCAGTT
AGTGCCGACACCTGATGGAAAAGGTCGTGTGGCGGCCATTGAGATTTTACTTAACTCGCCATTGGTACGCGACTTAATTC
AGCGCAATGAAATCGGTGCGCTAAAAGAAGCCATGAAAAAAGGCAAAGAGCAGGGCATGCAGAGTTTTGATATGGCGCTA
TACGATTTGTATAAAGCGGGCAAAATTGACATGGACCAGGCGCTTCATCATGCCGACTCGCCGAACGATTTACGCCTAAT
GATTAAGCTTGATACGAAAGACGGTTCAAGCTTGGGCACATTGTCTAATGTCTCAATTGACATGGATGACTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(6-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

60.647

100

0.608
  pilU Vibrio cholerae strain A1552

55.978

99.459

0.557
  pilU Acinetobacter baylyi ADP1

56.438

98.649

0.557
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.982

92.432

0.397
  pilT Acinetobacter nosocomialis M2

40

91.892

0.368
  pilT Acinetobacter baumannii D1279779

40

91.892

0.368
  pilT Acinetobacter baumannii strain A118

40

91.892

0.368