Detailed information NTG0763362

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: OXH74_RS18200
• Genome accession: NZ_CP113889
• Coordinates: 3889443..3890645 (+)
• Length: 400 a.a.
• NCBI ID: WP_375738217.1
• Uniprot ID: -
• Organism: Pseudomonas boanensis strain L337
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3884443..3895645

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
OXH74_RS18175 (OXH74_18145)	-	3884572..3886161 (-)	1590	WP_375738213.1	ABC-F family ATPase	-
OXH74_RS18180 (OXH74_18150)	-	3886775..3887203 (+)	429	WP_375738214.1	hydrogenase	-
OXH74_RS18185 (OXH74_18155)	-	3887296..3887553 (+)	258	WP_375738215.1	PRC-barrel domain-containing protein	-
OXH74_RS18190 (OXH74_18160)	-	3888310..3888525 (-)	216	WP_375738216.1	hypothetical protein	-
OXH74_RS18195 (OXH74_18165)	-	3889044..3889322 (-)	279	WP_215369699.1	peptidylprolyl isomerase	-
OXH74_RS18200 (OXH74_18170)	pilU	3889443..3890645 (+)	1203	WP_375738217.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
OXH74_RS18205 (OXH74_18175)	-	3890788..3891384 (+)	597	WP_375738218.1	DJ-1/PfpI family protein	-
OXH74_RS18210 (OXH74_18180)	-	3891407..3893362 (-)	1956	WP_375738219.1	acetoacetate--CoA ligase	-
OXH74_RS18215 (OXH74_18185)	-	3893417..3894331 (-)	915	WP_375738220.1	LysR family transcriptional regulator	-
OXH74_RS18220 (OXH74_18190)	-	3894457..3895155 (+)	699	WP_215370753.1	CoA transferase subunit A	-

Sequence

Protein

• Length: 400 a.a.
• Molecular weight: 44177.33 Da
• Isoelectric Point: 5.9332

>NTDB_id=763362 OXH74_RS18200 WP_375738217.1 3889443..3890645(+) (pilU) [Pseudomonas boanensis strain L337]
MDLSSMLKILATQDGSDLYLSTGAPPCAKFNGVLKPLSSEPLRAGDVAKIADSVMDAEQRAEFERELEMNLAISLPNIGR
FRINIFKQRNEVSIVARNIKLDIPKFEDLKLPEVLLKMVMEKRGLVLFVGGTGSGKSTSLAALIDYRNRNSGGHIITIED
PVEYVHRHKKSIINQREVGVDTRSFHAALKNTLRQAPDVILIGEIRDRETMEHALAFADTGHLAISTLHANNANQALDRI
INFFPEERRPQLLNDLGNNLKAFVSQRLVRTQDGKRRAAVEVLLGTSTISDLIKRGEFHSIKEIMEKSRGLGMQTFDQAL
FDLVVEGAISEDEAIKNADSANNLRLKLKLYREGPNGPAAQETTSTAAPAGGERAANWGLELKLEELEEDEPEEPGAGPR

Nucleotide

• Length: 1203 bp

>NTDB_id=763362 OXH74_RS18200 WP_375738217.1 3889443..3890645(+) (pilU) [Pseudomonas boanensis strain L337]
ATGGACCTTTCCTCGATGTTGAAAATCCTGGCCACCCAGGACGGTTCCGACCTTTACCTCTCTACTGGCGCGCCGCCCTG
CGCCAAATTCAACGGCGTGCTCAAACCGCTAAGCAGCGAGCCATTGCGCGCCGGCGACGTAGCAAAGATCGCCGATTCGG
TCATGGATGCCGAGCAGCGCGCTGAATTCGAGCGTGAGCTGGAGATGAACCTGGCCATTTCGCTGCCCAATATCGGCCGC
TTTCGCATCAATATCTTCAAACAGCGCAACGAGGTATCCATCGTCGCGCGGAACATCAAGCTCGATATCCCGAAATTCGA
GGACCTCAAACTGCCGGAAGTGCTGCTCAAGATGGTCATGGAGAAGCGCGGCCTGGTGTTGTTCGTCGGCGGCACCGGCT
CGGGCAAGTCCACCTCGCTGGCCGCACTGATCGACTACCGCAACCGCAATAGCGGCGGGCACATCATCACCATCGAAGAC
CCGGTGGAGTATGTGCATCGGCACAAGAAATCCATCATCAACCAGCGTGAAGTGGGCGTGGACACCCGCAGCTTCCATGC
AGCGTTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCCTGATCGGTGAGATCCGCGACCGCGAAACCATGGAGCACG
CCCTGGCTTTCGCCGACACCGGCCACCTGGCGATTTCCACATTGCATGCCAACAACGCCAACCAGGCGCTGGACCGTATC
ATCAACTTCTTCCCCGAGGAGCGTCGGCCGCAGCTGCTCAATGACCTGGGCAACAACCTCAAGGCCTTCGTTTCCCAGCG
TCTGGTGCGCACCCAGGATGGCAAACGCCGTGCGGCGGTTGAAGTGCTGCTCGGGACTTCGACCATCAGCGACCTGATCA
AGCGCGGTGAATTCCACTCCATCAAGGAAATCATGGAAAAGTCCCGTGGCCTGGGCATGCAGACCTTCGACCAGGCGTTG
TTCGACTTGGTGGTGGAGGGCGCCATCAGCGAGGACGAGGCGATCAAGAACGCCGACTCGGCCAACAATCTGCGCCTGAA
GCTCAAGCTTTATCGCGAGGGCCCCAATGGACCGGCTGCGCAGGAGACCACCTCGACAGCAGCGCCTGCTGGAGGCGAGA
GAGCTGCCAACTGGGGGCTCGAGCTGAAGTTGGAGGAGTTGGAGGAAGATGAGCCGGAGGAGCCGGGGGCAGGGCCGAGG
TAG

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-271)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	56.78	88.5	0.503
pilU	Acinetobacter baylyi ADP1	53.611	90	0.482
pilU	Vibrio cholerae strain A1552	52.355	90.25	0.473
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	39.323	96	0.378