Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilN   Type   Machinery gene
Locus tag   P7I81_RS09170 Genome accession   NZ_CP124648
Coordinates   1938220..1938816 (-) Length   198 a.a.
NCBI ID   WP_003095839.1    Uniprot ID   G3XD30
Organism   Pseudomonas aeruginosa strain 2020CK-00220     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1933220..1943816
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  P7I81_RS09150 (P7I81_09150) aroK 1934351..1934869 (-) 519 WP_003095833.1 shikimate kinase AroK -
  P7I81_RS09155 (P7I81_09155) pilQ 1934881..1937025 (-) 2145 WP_003095835.1 type 4a pilus secretin PilQ Machinery gene
  P7I81_RS09160 (P7I81_09160) comL/pilP 1937079..1937603 (-) 525 WP_003095836.1 type 4a pilus biogenesis lipoprotein PilP Machinery gene
  P7I81_RS09165 (P7I81_09165) pilO 1937600..1938223 (-) 624 WP_003103268.1 type 4a pilus biogenesis protein PilO Machinery gene
  P7I81_RS09170 (P7I81_09170) pilN 1938220..1938816 (-) 597 WP_003095839.1 type 4a pilus biogenesis protein PilN Machinery gene
  P7I81_RS09175 (P7I81_09175) pilM 1938816..1939880 (-) 1065 WP_003110888.1 type IV pilus assembly protein PilM Machinery gene
  P7I81_RS09180 (P7I81_09180) - 1940065..1942536 (+) 2472 WP_003095841.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 198 a.a.        Molecular weight: 22213.55 Da        Isoelectric Point: 10.0895

>NTDB_id=759449 P7I81_RS09170 WP_003095839.1 1938220..1938816(-) (pilN) [Pseudomonas aeruginosa strain 2020CK-00220]
MARINLLPWREELREQRKQQFLVILGGVLVASAALVFLGDQYFTAAIENQNARNDFLRKEIVVLDARIKEISELKSRRQQ
LLERMKIIQDLQGNRPIIGRVFDQLVRTLPDGVYFTDLKMTGKNIAIAGAAESNNRVSNLMRNMDASEWLTAPTLNEVKA
VTQGAVDQANVFQLTVQQTQPGEEDAKAKHGVAQGAKK

Nucleotide


Download         Length: 597 bp        

>NTDB_id=759449 P7I81_RS09170 WP_003095839.1 1938220..1938816(-) (pilN) [Pseudomonas aeruginosa strain 2020CK-00220]
ATGGCACGGATCAACCTTCTACCCTGGCGCGAAGAGCTGCGCGAGCAGCGCAAGCAGCAGTTCCTGGTGATTCTTGGCGG
CGTCCTGGTGGCATCGGCGGCGCTGGTATTCCTTGGCGACCAGTACTTCACCGCGGCCATCGAGAACCAGAACGCCCGCA
ACGACTTCCTGCGCAAGGAAATCGTCGTACTCGACGCCCGGATCAAGGAAATCAGCGAACTGAAGTCGCGTCGCCAGCAA
TTGCTCGAGCGGATGAAGATCATCCAGGACCTGCAGGGCAACCGTCCCATCATCGGACGGGTGTTCGACCAGCTCGTGCG
GACCTTGCCCGACGGCGTGTATTTCACCGACCTGAAGATGACCGGCAAGAACATTGCCATCGCCGGCGCGGCCGAGTCCA
ACAACCGCGTTTCCAATCTCATGCGCAACATGGACGCGTCCGAGTGGTTGACCGCCCCGACCCTGAACGAGGTCAAGGCG
GTGACCCAGGGCGCGGTGGACCAGGCCAACGTGTTCCAACTGACCGTGCAGCAGACGCAGCCCGGCGAGGAAGACGCCAA
GGCGAAGCATGGGGTTGCGCAAGGAGCCAAGAAATGA

Domains

Predicted by InterProScan.

Fimbrial assembly protein (PilN) (PilN)

(100-177)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  PDB 5EOU

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilN Legionella pneumophila strain ERS1305867

41.714

88.384

0.369