Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   OOL18_RS06990 Genome accession   NZ_CP110664
Coordinates   1498632..1499180 (-) Length   182 a.a.
NCBI ID   WP_237593742.1    Uniprot ID   A0A9Q4H8T0
Organism   Actinobacillus pleuropneumoniae strain AP_123     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1493632..1504180
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OOL18_RS06970 - 1494020..1495306 (+) 1287 WP_005615681.1 NAD(P)/FAD-dependent oxidoreductase -
  OOL18_RS06975 - 1495498..1496715 (-) 1218 Protein_1367 helix-hairpin-helix domain-containing protein -
  OOL18_RS06980 - 1496756..1497409 (+) 654 WP_103034748.1 IS1595 family transposase -
  OOL18_RS06985 - 1497430..1498557 (-) 1128 Protein_1369 Tex-like N-terminal domain-containing protein -
  OOL18_RS06990 pilF 1498632..1499180 (-) 549 WP_237593742.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  OOL18_RS06995 - 1499234..1500415 (-) 1182 WP_005608495.1 bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN -
  OOL18_RS07020 gltX 1501319..1502758 (+) 1440 WP_005608501.1 glutamate--tRNA ligase -
  OOL18_RS07030 yefM 1503096..1503347 (+) 252 WP_005598318.1 YoeB-YefM toxin-antitoxin system antitoxin YefM -
  OOL18_RS07035 - 1503344..1503604 (+) 261 WP_005605064.1 Txe/YoeB family addiction module toxin -
  OOL18_RS07040 - 1503763..1503930 (-) 168 WP_005598320.1 Trm112 family protein -

Sequence

Protein


Download         Length: 182 a.a.        Molecular weight: 20628.24 Da        Isoelectric Point: 6.9477

>NTDB_id=755830 OOL18_RS06990 WP_237593742.1 1498632..1499180(-) (pilF) [Actinobacillus pleuropneumoniae strain AP_123]
MNLAKFLRNLSACMLLSVLFGCSSQLPTSQTTFNRSEAVKARINLALAYLDQHDLPKAKENIDKALSHDQNDYLPHSVLA
YYYQQIGDQVHAEQAYQQALKLSNNRPDVLNNYGTFLCKQAKFDQAYQQFEQAVQSEQPYYHQADSLENIVLCAKQEPNL
AKANEALSQLEKLDKSRAAALR

Nucleotide


Download         Length: 549 bp        

>NTDB_id=755830 OOL18_RS06990 WP_237593742.1 1498632..1499180(-) (pilF) [Actinobacillus pleuropneumoniae strain AP_123]
ATGAATCTTGCAAAATTTTTGCGAAATTTGAGCGCTTGTATGCTGTTATCCGTGTTGTTCGGTTGTTCTTCTCAGCTTCC
AACCTCCCAAACTACCTTTAATCGTTCGGAAGCGGTTAAAGCCCGTATTAATTTAGCCTTAGCGTATCTCGATCAACATG
ATTTACCCAAAGCGAAAGAGAATATTGATAAAGCGCTAAGCCACGATCAAAACGATTACCTACCTCACTCCGTTTTAGCT
TATTATTATCAACAAATCGGTGATCAAGTTCACGCCGAGCAAGCTTATCAGCAAGCATTGAAGCTTAGTAATAACCGTCC
GGATGTCTTAAATAATTACGGTACTTTTCTGTGTAAACAAGCCAAATTTGATCAGGCATATCAGCAGTTTGAGCAAGCGG
TGCAAAGCGAACAACCTTATTATCATCAAGCCGATAGCCTCGAAAATATTGTGTTATGTGCCAAACAAGAGCCGAATTTA
GCTAAAGCAAATGAAGCGTTAAGCCAATTAGAAAAGCTGGATAAATCAAGAGCGGCGGCACTACGCTAG

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_8)

(79-105)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Glaesserella parasuis strain SC1401

67.553

100

0.698
  pilF/pilF2 Haemophilus influenzae Rd KW20

47.368

93.956

0.445