Detailed information    

insolico Bioinformatically predicted

Overview

Name   comE   Type   Machinery gene
Locus tag   OOL18_RS01045 Genome accession   NZ_CP110664
Coordinates   224543..225841 (+) Length   432 a.a.
NCBI ID   WP_265410929.1    Uniprot ID   -
Organism   Actinobacillus pleuropneumoniae strain AP_123     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 219543..230841
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OOL18_RS01020 - 219715..222282 (-) 2568 WP_005595965.1 penicillin-binding protein 1A -
  OOL18_RS01025 - 222421..223101 (+) 681 WP_265410928.1 pilus assembly protein PilM -
  OOL18_RS01030 - 223083..223604 (+) 522 WP_005595970.1 hypothetical protein -
  OOL18_RS01035 - 223703..224134 (+) 432 WP_005595972.1 hypothetical protein -
  OOL18_RS01040 - 224127..224531 (+) 405 WP_005595974.1 hypothetical protein -
  OOL18_RS01045 comE 224543..225841 (+) 1299 WP_265410929.1 type IV pilus secretin PilQ Machinery gene
  OOL18_RS01050 nusB 225967..226380 (+) 414 WP_005595979.1 transcription antitermination factor NusB -
  OOL18_RS01055 thiL 226445..227410 (+) 966 WP_005618774.1 thiamine-phosphate kinase -
  OOL18_RS01060 - 227423..227896 (+) 474 WP_005603437.1 phosphatidylglycerophosphatase A -
  OOL18_RS01065 - 227909..228535 (+) 627 WP_039709529.1 LysE family transporter -
  OOL18_RS01070 - 228583..229620 (-) 1038 WP_005603442.1 TrmH family RNA methyltransferase -
  OOL18_RS01075 suhB 229786..230598 (+) 813 WP_005595989.1 inositol-1-monophosphatase -

Sequence

Protein


Download         Length: 432 a.a.        Molecular weight: 47779.90 Da        Isoelectric Point: 9.4227

>NTDB_id=755819 OOL18_RS01045 WP_265410929.1 224543..225841(+) (comE) [Actinobacillus pleuropneumoniae strain AP_123]
MRKMIWLLLFILTPVMANTFSLSLKNAPTAEILSYLAEEYGKNIVLSDNIETNTTLRIENSDFDSVLKSITRANKLTSAY
EKQIYFIGHKKDEKAATAIGVNSDLLKPKLITKTIKLDYAKAAEVIESLTKGSGHFLSENGYLHFDDRSNSLIIKDSPES
MKNIVQLIRNLDKPTEQIAIEARIVTISSENLQELGVRWGIFAPTNGHHKIAGSLEANGLPNTNRLNVNFPVNNAASIAL
QVAKINGRVLDLELTALEQENNVEIIASPRLLTTNKKPASIKQGTEVPYVLYNRKDEVKNIEFKEAVLGLQVTPHISNDK
QILLDLVVTQNSTNSTGSTLQGLVTIDKQELNTQVFAQHGETIVLGGIFQHLTAKGEDRVPILGSIPVLKKLFSHSSDKI
SKRELVIFVTPYIVKNGQPQISSHSSQKLAPK

Nucleotide


Download         Length: 1299 bp        

>NTDB_id=755819 OOL18_RS01045 WP_265410929.1 224543..225841(+) (comE) [Actinobacillus pleuropneumoniae strain AP_123]
ATGCGAAAAATGATTTGGTTGCTCCTGTTTATATTAACGCCGGTTATGGCAAATACTTTTTCTCTCTCGCTGAAAAATGC
GCCGACTGCGGAAATTTTAAGTTATTTAGCCGAAGAATACGGTAAAAATATCGTGTTAAGCGACAATATTGAGACAAATA
CTACATTAAGAATTGAAAATAGTGATTTTGACAGCGTATTAAAAAGTATTACTCGGGCGAATAAACTCACGAGTGCATAC
GAAAAACAGATCTATTTTATCGGGCATAAAAAAGATGAGAAAGCGGCTACGGCAATAGGCGTAAATTCTGATTTATTGAA
ACCTAAATTGATCACTAAGACAATAAAATTAGATTATGCGAAGGCGGCGGAAGTGATTGAATCTTTAACTAAAGGAAGCG
GCCATTTTTTATCGGAAAACGGCTATTTGCATTTTGATGATCGTAGTAACAGTTTGATTATTAAAGACAGTCCGGAATCG
ATGAAAAATATCGTGCAATTAATCAGAAACTTAGATAAACCGACCGAACAGATTGCAATTGAAGCCAGAATTGTCACGAT
AAGCAGTGAGAATTTACAAGAATTAGGTGTGCGTTGGGGGATATTTGCTCCTACAAACGGACACCATAAAATTGCCGGTT
CGCTTGAAGCGAACGGATTACCGAATACTAATCGTTTAAACGTAAATTTTCCGGTAAATAATGCCGCTTCTATTGCACTA
CAAGTGGCGAAAATTAATGGGCGAGTGCTTGATTTGGAACTTACCGCTTTGGAACAAGAAAATAATGTTGAGATTATCGC
CAGCCCTCGTTTATTAACCACCAATAAGAAGCCGGCAAGCATTAAGCAAGGAACGGAAGTTCCTTATGTGCTGTATAACC
GTAAAGACGAAGTGAAAAATATCGAATTTAAAGAAGCGGTTTTAGGTTTACAAGTGACGCCGCATATTTCAAACGATAAG
CAAATTTTGCTCGATTTGGTAGTCACCCAAAACTCAACTAATTCGACCGGTTCAACCCTACAAGGCTTAGTGACGATTGA
TAAGCAGGAACTGAATACGCAAGTATTTGCTCAGCATGGCGAAACGATTGTGCTAGGCGGTATTTTTCAGCATTTAACCG
CAAAAGGTGAGGACAGAGTGCCGATTTTAGGTTCGATTCCGGTACTTAAAAAGTTATTTAGCCATTCTAGTGATAAAATT
AGTAAGCGTGAACTAGTGATTTTTGTGACGCCTTACATTGTTAAAAACGGACAACCGCAAATTTCCTCACATTCTTCACA
GAAATTAGCGCCAAAATAG

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(112-177)

Bacterial type II and III secretion system protein (Secretin)

(256-415)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comE Glaesserella parasuis strain SC1401

66.265

96.065

0.637
  comE Haemophilus influenzae Rd KW20

52.804

99.074

0.523
  comE Haemophilus influenzae 86-028NP

52.57

99.074

0.521
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

42.41

96.065

0.407
  pilQ Vibrio cholerae strain A1552

42.41

96.065

0.407
  pilQ Vibrio campbellii strain DS40M4

40.093

99.306

0.398