Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   QDY55_RS04240 Genome accession   NZ_CP122768
Coordinates   878296..879789 (+) Length   497 a.a.
NCBI ID   WP_000249333.1    Uniprot ID   -
Organism   Escherichia coli strain ETEC1738     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 873296..884789
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QDY55_RS04220 (QDY55_04215) pilD 873944..874753 (+) 810 WP_064225894.1 prepilin peptidase PppA Machinery gene
  QDY55_RS04225 (QDY55_04220) gspS2 874819..875229 (+) 411 WP_001309757.1 type II secretion system pilot lipoprotein GspS-beta -
  QDY55_RS04230 (QDY55_04225) gspC 875247..876206 (+) 960 WP_149449034.1 type II secretion system protein GspC -
  QDY55_RS04235 (QDY55_04230) gspD 876236..878296 (+) 2061 WP_000498829.1 type II secretion system secretin GspD -
  QDY55_RS04240 (QDY55_04235) pilB/pilB1 878296..879789 (+) 1494 WP_000249333.1 type II secretion system ATPase GspE Machinery gene
  QDY55_RS04245 (QDY55_04240) gspF 879789..881012 (+) 1224 WP_001173400.1 type II secretion system inner membrane protein GspF -
  QDY55_RS04250 (QDY55_04245) gspG 881029..881484 (+) 456 WP_001087292.1 type II secretion system major pseudopilin GspG -
  QDY55_RS04255 (QDY55_04250) gspH 881488..882051 (+) 564 WP_001115158.1 type II secretion system minor pseudopilin GspH -
  QDY55_RS04260 (QDY55_04255) gspI 882048..882419 (+) 372 WP_089592173.1 type II secretion system minor pseudopilin GspI -
  QDY55_RS04265 (QDY55_04260) gspJ 882416..883021 (+) 606 WP_032084338.1 type II secretion system minor pseudopilin GspJ -
  QDY55_RS04270 (QDY55_04265) gspK 883018..883995 (+) 978 WP_149449088.1 type II secretion system minor pseudopilin GspK -

Sequence

Protein


Download         Length: 497 a.a.        Molecular weight: 55272.43 Da        Isoelectric Point: 6.0089

>NTDB_id=742905 QDY55_RS04240 WP_000249333.1 878296..879789(+) (pilB/pilB1) [Escherichia coli strain ETEC1738]
MVPVAQETTANTVRLPYSFSRRFSLVAWCEASLEILHVHPLSLSVLQELQRGLNAPFTLRQIDEAEFEQRLNAVWQRDSS
EARQLMEDLGSAEDFFTLAEELPETEDLLESDDDAPIIKLINAMLAEAIKEGASDIHIETFEKSLVIRFRVDGTLHEMLR
PGRKLASLLVSRIKVMARLDIAEKRVPQDGRIALLLGGRAIDVRVSTMPSAWGERVVLRLLDKNQARLTLERLGLSHELT
AQLRQLLHKPHGIFLVTGPTGSGKSTTLYAGLQELNNHSRNILTVEDPIEYMIEGIGQTQVNTRVGMTFARGLRAILRQD
PDVVMVGEIRDTETAEIAVQASLTGHLVLSTLHTNTAVGAITRLQDMGVEPFLLSSSLTGVMAQRLVRTLCPDCRQPVPA
TDEEKRLLGITDARTVTLYHPQGCPACNHKGFRGRTAIHELIVVDATLRDLIHRQAGELELERYVRQHSTGIRSNGIEKV
LAGETSLDEVLRVTMEA

Nucleotide


Download         Length: 1494 bp        

>NTDB_id=742905 QDY55_RS04240 WP_000249333.1 878296..879789(+) (pilB/pilB1) [Escherichia coli strain ETEC1738]
ATGGTGCCTGTAGCACAGGAAACCACCGCTAACACCGTGCGTCTGCCCTACAGTTTCAGCCGTCGGTTTAGCCTGGTGGC
ATGGTGCGAAGCGTCGCTGGAGATCCTCCACGTTCATCCGCTATCGCTCTCTGTTTTGCAGGAGCTGCAGCGGGGGCTGA
ACGCGCCCTTTACGCTGCGGCAAATCGACGAAGCCGAATTTGAACAGCGGCTGAATGCGGTCTGGCAGCGGGACTCTTCC
GAGGCCCGCCAGTTGATGGAAGATCTCGGTTCTGCTGAGGATTTTTTTACCCTCGCCGAAGAACTGCCGGAAACGGAAGA
TCTGCTGGAAAGCGACGACGACGCGCCAATCATCAAACTGATCAACGCTATGCTGGCAGAGGCGATTAAAGAAGGCGCTT
CGGATATCCACATCGAGACATTTGAAAAGAGTCTGGTGATCCGTTTTCGCGTTGACGGCACATTACATGAAATGTTGCGC
CCCGGTCGCAAGCTGGCCTCGCTGCTGGTCTCGCGTATCAAGGTGATGGCGCGGCTGGATATCGCCGAAAAGCGCGTACC
GCAGGATGGACGTATTGCGCTGCTGCTGGGCGGTCGGGCGATTGACGTGCGTGTCTCCACCATGCCATCCGCCTGGGGCG
AGCGCGTGGTGCTGCGACTGCTGGACAAAAACCAGGCCCGCCTGACGCTGGAGCGTCTGGGGTTGAGCCATGAACTGACC
GCGCAGTTGCGCCAGCTGTTACACAAACCGCACGGTATCTTTCTGGTTACGGGGCCGACGGGTTCCGGCAAAAGCACCAC
GCTGTACGCCGGATTGCAGGAGCTGAACAACCACTCGCGTAACATTCTCACGGTTGAAGACCCCATCGAATACATGATTG
AAGGGATCGGTCAGACGCAGGTTAACACCCGCGTTGGGATGACCTTCGCCCGTGGCCTGCGCGCGATTTTGCGTCAGGAC
CCGGATGTGGTGATGGTCGGTGAAATCCGCGATACCGAAACCGCAGAAATCGCCGTTCAGGCTTCACTTACCGGACACCT
GGTCCTTTCCACGCTGCATACCAACACAGCGGTGGGGGCGATCACACGTTTGCAGGATATGGGCGTGGAGCCTTTCCTGC
TCTCTTCCAGTCTGACGGGCGTGATGGCGCAGCGACTGGTTCGCACGCTGTGCCCCGACTGCCGCCAGCCCGTGCCTGCC
ACCGACGAAGAAAAACGCCTGCTGGGGATTACCGATGCGCGAACCGTCACTCTGTACCATCCGCAGGGCTGTCCCGCCTG
TAATCACAAAGGCTTTCGCGGACGTACCGCCATCCATGAGCTGATCGTGGTGGACGCCACATTGCGTGATTTGATCCACC
GTCAGGCCGGGGAACTGGAGCTGGAACGTTATGTCCGGCAACACTCTACGGGTATCCGCAGCAACGGCATTGAGAAAGTG
CTCGCCGGAGAAACCTCTCTCGATGAAGTTCTGCGGGTAACCATGGAGGCGTAA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(123-389)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

39.556

100

0.431
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

47.765

85.513

0.408
  pilB Vibrio parahaemolyticus RIMD 2210633

48.363

79.879

0.386
  pilB Vibrio campbellii strain DS40M4

47.475

79.678

0.378
  pilB Legionella pneumophila strain ERS1305867

43.619

86.72

0.378
  pilB Acinetobacter baumannii D1279779

46.366

80.282

0.372
  pilB Vibrio cholerae strain A1552

46.212

79.678

0.368
  pilF Thermus thermophilus HB27

45.25

80.483

0.364