Detailed information NTG0740845

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: OIO53_RS04370
• Genome accession: NZ_CP107579
• Coordinates: 924481..925599 (-)
• Length: 372 a.a.
• NCBI ID: WP_000347036.1
• Uniprot ID: A0A009PAD6
• Organism: Acinetobacter baumannii strain 1326525-1
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 919481..930599

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
OIO53_RS04340 (OIO53_04340)	-	920310..921158 (+)	849	WP_000336551.1	class II glutamine amidotransferase	-
OIO53_RS04345 (OIO53_04345)	-	921411..921866 (+)	456	WP_000782976.1	bacteriohemerythrin	-
OIO53_RS04350 (OIO53_04350)	-	922019..923086 (+)	1068	WP_031959810.1	membrane protein	-
OIO53_RS04355 (OIO53_04355)	-	923086..923409 (+)	324	WP_000442585.1	RnfH family protein	-
OIO53_RS04360 (OIO53_04360)	-	923464..923862 (-)	399	WP_001170994.1	outer membrane protein assembly factor BamE	-
OIO53_RS04365 (OIO53_04365)	fur	923974..924411 (+)	438	WP_001122847.1	ferric iron uptake transcriptional regulator	-
OIO53_RS04370 (OIO53_04370)	pilU	924481..925599 (-)	1119	WP_000347036.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
OIO53_RS04375 (OIO53_04375)	pilT	925627..926664 (-)	1038	WP_000355489.1	type IV pilus twitching motility protein PilT	Machinery gene
OIO53_RS04380 (OIO53_04380)	-	926791..927483 (+)	693	WP_031959811.1	YggS family pyridoxal phosphate-dependent enzyme	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41905.11 Da
• Isoelectric Point: 6.6944

>NTDB_id=740845 OIO53_RS04370 WP_000347036.1 924481..925599(-) (pilU) [Acinetobacter baumannii strain 1326525-1]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRKEFAETRECNFAIMNREKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLADEGPDQLSDGKQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=740845 OIO53_RS04370 WP_000347036.1 924481..925599(-) (pilU) [Acinetobacter baumannii strain 1326525-1]
ATGGATTTTAATGACCTGCTCAACCTGATGGTTGAAAAGAAATCATCGGATCTCTTTATTACAGATGGCGTTGCGCCCTC
TATGAAGATTAATGGGCAAATTGTTCCAATTTCAAAAAACAGTCTTTCTGGGGAAGTGATTGGTCAACTATTACATTCCA
TCATGAGTGAAAAACAACGCAAAGAATTTGCAGAAACTCGTGAATGTAACTTTGCCATTATGAACCGTGAAAAAACGGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAGCGCGACATGCCGGGCATGGTACTACGTCGTATTGAAACCAAGATTCCTTCAAT
TGATGACTTACAGTTACCACCTGTACTTAAAGATTTATCGATGACCAAACGTGGCATTATTATTTTCGTAGGCGCGACAG
GTACAGGTAAATCTACTTCATTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAGGGCCATATCATTACCATTGAA
GACCCGATTGAGTTTATTCACGAACATGCAGGTTGCATCATTACTCAGCGTGAAGTCGGGATCGATACCGACTCATTTGA
AATTGCCTTGAAAAATACACTACGACAAGCGCCGGATGTGATCTTAATTGGTGAGATCCGTTCTCGTGAAGTCATGGACT
ACGCGATTGGCTTTGCTGAAACAGGTCACCTTGTATTAGCTACCATGCACGCCAACAACGCCAACCAAGCGCTCGACCGT
ATTATTCACTTTTTTGAAAGTGACCGTCACAGCCAGCTTTATATGGACTTGTCACTTAACTTAAAGGCTATGATTGCGCA
GCAGCTCATTCCAACACCAGATGGTAATTCACGCCGTGCAGCAATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAGGATTTGATGAAACGCTCACGTGAACTTGGTATGCAGACCTTTGACCAA
GCTTTATTTGATTTATATAAAGCTGGTCAAATTACCTACAAAGATGCACTTAAACATGCTGACTCACCGAACGATTTACG
TTTAACCATTAAACTTGCTGATGAGGGTCCTGATCAATTATCAGACGGGAAACAGCATTTAACTTTTGACCGCCAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (31-270)

3D structure

• AlphaFold DB: A0A009PAD6

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.926	100	0.772
pilU	Pseudomonas stutzeri DSM 10701	69.293	98.925	0.685
pilU	Vibrio cholerae strain A1552	53.243	99.462	0.53
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.197	93.011	0.392