Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   OFU58_RS17415 Genome accession   NZ_CP107289
Coordinates   3588825..3590537 (+) Length   570 a.a.
NCBI ID   WP_280586239.1    Uniprot ID   -
Organism   Acinetobacter pittii strain BM4623     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3583825..3595537
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OFU58_RS17380 (OFU58_17385) nusA 3584713..3586197 (-) 1485 WP_002115134.1 transcription termination factor NusA -
  OFU58_RS17385 (OFU58_17390) rimP 3586235..3586759 (-) 525 WP_002114922.1 ribosome maturation factor RimP -
  OFU58_RS17405 (OFU58_17410) secG 3587397..3587726 (-) 330 WP_002115012.1 preprotein translocase subunit SecG -
  OFU58_RS17410 (OFU58_17415) tpiA 3587739..3588533 (-) 795 WP_068565290.1 triose-phosphate isomerase -
  OFU58_RS17415 (OFU58_17420) pilB 3588825..3590537 (+) 1713 WP_280586239.1 type IV-A pilus assembly ATPase PilB Machinery gene
  OFU58_RS17420 (OFU58_17425) pilC 3590564..3591790 (+) 1227 WP_002114869.1 type II secretion system F family protein Machinery gene
  OFU58_RS17425 (OFU58_17430) pilD 3591790..3592650 (+) 861 WP_280586252.1 A24 family peptidase Machinery gene
  OFU58_RS17430 (OFU58_17435) coaE 3592652..3593248 (+) 597 WP_206216940.1 dephospho-CoA kinase -
  OFU58_RS17435 (OFU58_17440) - 3593245..3594159 (-) 915 WP_069036505.1 DMT family transporter -
  OFU58_RS17440 (OFU58_17445) rlmB 3594195..3594944 (-) 750 WP_005079348.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  OFU58_RS17445 (OFU58_17450) - 3595047..3595373 (-) 327 WP_002115102.1 pyrimidine/purine nucleoside phosphorylase -

Sequence

Protein


Download         Length: 570 a.a.        Molecular weight: 63599.95 Da        Isoelectric Point: 5.0932

>NTDB_id=739354 OFU58_RS17415 WP_280586239.1 3588825..3590537(+) (pilB) [Acinetobacter pittii strain BM4623]
MSVIHTSPKFSGFFRRLVEEKHVSAATMQTALDAAKRAKRDTVAYLIEEVHISPSLLAETISIEFAEPYFDLDVYDTSQI
PKDLVDQKLVLKHRVLPLIQRGQILYVATSNPSNIEAIDAIRFNSKLMVEPVIVEHHKLEKILTQQFTEESNFEFSDEEF
DLDVDLEAPSSQEEEEETPQGDEAPIVKYINKLLIDAIRMGASDLHFEPYEKSYRVRYRVDGVLRQIANPPLQLANRLAS
RLKVMSQMDISEKRVPQDGRIKLKLSKTKAIDFRVNSLPTLFGEKLVLRILDPSSAMLGIDALGYEEDQKALFLEALDKP
QGMLLITGPTGSGKTVSLYTGLNILNTESSNISTAEDPVEINLEGINQVNVNPKVGLTFSAALKSFLRQDPDIIMVGEIR
DLETAEIAIKAAQTGHMVMSTLHTNSAPETLTRLRNMGVPSFNIATSVNLVIAQRLARRLCPQCKTPADIPKQSLLEMGF
TDQDLTHPDFQVFQPVGCTECREGYKGRVGIYEVMKVTPEISKIIMEDGNALEIAAASEKLGFNNLRRSGLKKVMQGVTS
LQEVNRVTSE

Nucleotide


Download         Length: 1713 bp        

>NTDB_id=739354 OFU58_RS17415 WP_280586239.1 3588825..3590537(+) (pilB) [Acinetobacter pittii strain BM4623]
ATGTCAGTCATACATACATCTCCAAAATTTTCGGGGTTTTTTCGACGATTAGTCGAAGAAAAACATGTGTCGGCAGCGAC
CATGCAAACAGCATTAGATGCAGCAAAGAGAGCTAAACGGGATACGGTTGCGTACCTGATTGAAGAGGTTCATATCTCCC
CTTCTTTATTAGCTGAAACTATTTCTATTGAATTTGCTGAACCATATTTTGATCTGGATGTTTACGATACCAGCCAAATT
CCCAAGGATTTAGTCGATCAGAAACTGGTTTTAAAACATCGGGTCTTACCTCTCATACAAAGAGGACAGATTTTATATGT
CGCGACCAGCAACCCGAGTAATATTGAAGCGATTGATGCCATTCGCTTTAATAGCAAGCTCATGGTTGAACCAGTTATTG
TCGAGCACCATAAGCTTGAGAAAATTCTGACGCAGCAATTTACCGAAGAAAGCAACTTCGAGTTTAGTGATGAAGAGTTT
GATCTTGATGTAGACCTTGAAGCTCCATCTTCACAAGAAGAGGAGGAAGAAACGCCTCAAGGCGATGAAGCACCTATTGT
TAAATATATTAATAAATTACTCATCGATGCGATTCGTATGGGGGCTTCAGACTTACATTTTGAACCATACGAGAAATCAT
ATCGGGTCCGTTACCGAGTCGACGGCGTGTTGCGTCAGATTGCAAATCCACCTTTGCAATTGGCTAACCGTTTGGCTTCA
CGTTTAAAAGTCATGTCTCAAATGGATATTTCCGAAAAACGAGTACCTCAAGATGGTCGTATCAAGCTTAAGTTATCAAA
GACTAAAGCAATCGATTTTCGTGTGAACTCACTTCCAACTTTGTTTGGTGAAAAACTGGTTCTGCGTATTCTCGATCCTT
CTAGCGCAATGCTCGGAATTGATGCTTTAGGATATGAAGAAGATCAGAAAGCTCTTTTTTTAGAAGCACTCGATAAACCA
CAAGGTATGCTTTTAATTACAGGTCCTACCGGTTCTGGTAAAACGGTATCTTTATATACAGGCTTAAATATTTTAAATAC
TGAAAGTTCGAATATTTCCACTGCCGAAGATCCAGTCGAAATCAACCTTGAAGGAATTAATCAGGTTAACGTCAACCCGA
AAGTAGGTCTGACTTTTTCTGCTGCACTTAAATCATTTTTACGTCAAGATCCGGACATTATTATGGTCGGTGAGATTCGT
GATTTAGAAACGGCTGAAATTGCGATTAAGGCTGCACAAACCGGTCATATGGTCATGTCCACACTGCATACCAATAGTGC
TCCAGAAACACTAACTCGCCTACGCAATATGGGAGTTCCATCCTTTAATATCGCAACATCAGTCAACTTGGTTATTGCAC
AGCGTTTAGCACGTCGGCTATGCCCGCAGTGTAAAACACCTGCCGACATTCCTAAACAGAGCCTATTAGAAATGGGCTTT
ACTGACCAAGATTTAACTCATCCCGATTTTCAAGTTTTCCAGCCTGTCGGTTGTACTGAGTGTCGTGAAGGCTATAAAGG
TCGAGTAGGTATTTACGAGGTAATGAAAGTTACACCTGAAATTTCCAAGATTATTATGGAAGATGGCAATGCTTTAGAAA
TCGCTGCTGCTTCTGAAAAACTGGGGTTTAATAATCTGCGTCGTTCAGGATTAAAGAAAGTCATGCAAGGTGTTACTTCC
TTACAAGAAGTCAACCGCGTGACCAGTGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(192-459)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-145)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

94.386

100

0.944
  pilB Acinetobacter baylyi ADP1

78.07

100

0.781
  pilB Legionella pneumophila strain ERS1305867

50.794

99.474

0.505
  pilF Neisseria gonorrhoeae MS11

49.644

98.596

0.489
  pilB Vibrio parahaemolyticus RIMD 2210633

51.132

92.982

0.475
  pilB Vibrio campbellii strain DS40M4

50.867

91.053

0.463
  pilB Vibrio cholerae strain A1552

50.096

91.053

0.456
  pilB/pilB1 Synechocystis sp. PCC 6803

34.691

100

0.374