Detailed information    

insolico Bioinformatically predicted

Overview


Name   pilE   Type   Machinery gene
Locus tag   OD459_RS27450 Genome accession   NZ_CP107027
Coordinates   3249657..3249764 (+) Length   35 a.a.
NCBI ID   WP_412059427.1    Uniprot ID   -
Organism   Cytobacillus firmus strain M7     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE


Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 3249657..3263407 3249657..3249764 within 0


Gene organization within MGE regions


Location: 3249657..3263407
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OD459_RS27450 pilE 3249657..3249764 (+) 108 WP_412059427.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  OD459_RS16645 (OD459_16645) - 3250338..3250973 (+) 636 WP_263599306.1 sugar transferase -
  OD459_RS16650 (OD459_16650) - 3250970..3251821 (+) 852 WP_263599307.1 NAD-dependent epimerase/dehydratase family protein -
  OD459_RS16655 (OD459_16655) - 3251823..3252929 (+) 1107 WP_263599308.1 glycosyltransferase family 4 protein -
  OD459_RS16660 (OD459_16660) - 3252922..3253482 (+) 561 WP_263599309.1 acyltransferase -
  OD459_RS16665 (OD459_16665) - 3253504..3254664 (+) 1161 WP_263599310.1 glycosyltransferase -
  OD459_RS16670 (OD459_16670) - 3254671..3254860 (+) 190 Protein_3217 polysaccharide biosynthesis protein -
  OD459_RS16675 (OD459_16675) - 3255245..3256309 (+) 1065 WP_263599311.1 EpsG family protein -
  OD459_RS16680 (OD459_16680) - 3256316..3257527 (+) 1212 WP_263599312.1 glycosyltransferase family 4 protein -
  OD459_RS16685 (OD459_16685) - 3257612..3258748 (+) 1137 WP_263599313.1 glycosyltransferase -
  OD459_RS16690 (OD459_16690) - 3258750..3260096 (+) 1347 WP_263599314.1 hypothetical protein -
  OD459_RS16695 (OD459_16695) - 3260113..3261150 (+) 1038 WP_263599315.1 nucleoside-diphosphate sugar epimerase/dehydratase -
  OD459_RS16700 (OD459_16700) - 3261167..3262276 (+) 1110 WP_263599316.1 capsular polysaccharide biosynthesis protein CapF -
  OD459_RS16705 (OD459_16705) wecB 3262280..3263407 (+) 1128 WP_263599317.1 non-hydrolyzing UDP-N-acetylglucosamine 2-epimerase -

Sequence


Protein


Download         Length: 35 a.a.        Molecular weight: 3762.56 Da        Isoelectric Point: 4.1051

>NTDB_id=737475 OD459_RS27450 WP_412059427.1 3249657..3249764(+) (pilE) [Cytobacillus firmus strain M7]
MEINKDKGFTLIEVIMVIAIVGIIAGISLPSVVYD

Nucleotide


Download         Length: 108 bp        

>NTDB_id=737475 OD459_RS27450 WP_412059427.1 3249657..3249764(+) (pilE) [Cytobacillus firmus strain M7]
ATGGAGATAAATAAAGATAAAGGATTTACTTTGATAGAAGTAATAATGGTTATCGCTATTGTTGGAATAATTGCAGGAAT
TTCTTTGCCTTCTGTAGTGTATGATTGA


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Vibrio campbellii strain DS40M4

50

97.143

0.486

  pilE Neisseria gonorrhoeae strain FA1090

68

71.429

0.486

  pilE Neisseria gonorrhoeae MS11

68

71.429

0.486

  pilA Ralstonia pseudosolanacearum GMI1000

64

71.429

0.457

  pilX Neisseria meningitidis 8013

59.259

77.143

0.457

  pilL Neisseria gonorrhoeae MS11

59.259

77.143

0.457

  pilH Neisseria gonorrhoeae MS11

46.875

91.429

0.429

  pilV Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50

85.714

0.429

  comF Acinetobacter baylyi ADP1

62.5

68.571

0.429

  pilY2 Acinetobacter baumannii D1279779

57.692

74.286

0.429

  fimT Acinetobacter baylyi ADP1

54.167

68.571

0.371

  pilA Vibrio cholerae strain A1552

50

74.286

0.371

  pilA Vibrio cholerae O1 biovar El Tor strain E7946

50

74.286

0.371

  pilA/pilA1 Synechocystis sp. PCC 6803

50

74.286

0.371

  pilA Vibrio cholerae C6706

50

74.286

0.371

  pilA Vibrio parahaemolyticus RIMD 2210633

48.148

77.143

0.371

  pilV Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

48.148

77.143

0.371