Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   M9799_RS09410 Genome accession   NZ_CP106881
Coordinates   2102800..2104371 (+) Length   523 a.a.
NCBI ID   WP_231041427.1    Uniprot ID   -
Organism   Comamonas endophytica strain 5MLIR     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2097800..2109371
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M9799_RS09385 (M9799_09385) argS 2098096..2099793 (+) 1698 WP_231041422.1 arginine--tRNA ligase -
  M9799_RS09390 (M9799_09390) - 2099806..2100477 (+) 672 WP_231041423.1 SPOR domain-containing protein -
  M9799_RS09395 (M9799_09395) - 2100532..2101182 (+) 651 WP_231041424.1 thiol:disulfide interchange protein DsbA/DsbL -
  M9799_RS09400 (M9799_09400) lptA 2101354..2101977 (+) 624 WP_231041425.1 lipopolysaccharide transport periplasmic protein LptA -
  M9799_RS09405 (M9799_09405) lptB 2101974..2102756 (+) 783 WP_231041426.1 LPS export ABC transporter ATP-binding protein -
  M9799_RS09410 (M9799_09410) rpoN/rpoN1 2102800..2104371 (+) 1572 WP_231041427.1 RNA polymerase factor sigma-54 Machinery gene
  M9799_RS09415 (M9799_09415) - 2104442..2105719 (+) 1278 WP_231041428.1 THUMP domain-containing protein -
  M9799_RS09420 (M9799_09420) - 2105716..2106240 (+) 525 WP_231041429.1 PIN domain-containing protein -
  M9799_RS09425 (M9799_09425) - 2106380..2107231 (+) 852 WP_231041430.1 UPF0149 family protein -
  M9799_RS09430 (M9799_09430) - 2107337..2108800 (+) 1464 WP_231041431.1 SulP family inorganic anion transporter -

Sequence

Protein


Download         Length: 523 a.a.        Molecular weight: 57851.88 Da        Isoelectric Point: 4.7862

>NTDB_id=736408 M9799_RS09410 WP_231041427.1 2102800..2104371(+) (rpoN/rpoN1) [Comamonas endophytica strain 5MLIR]
MKPGLSLRVSQHLALTPQLQQSIWLLQLSTLELSQEVEHMLDDNPFLERTTEEAEREEFGLAQSDVPAQRDDYAEDAQHA
SAEMSGSSGDADAGEVTAPADAAPDWEGDGSSDLAPDDSEWGGEARARNGSSEDDAEAQDLAHGHESLTDHLHRQALSLR
LGEVDRAALRFLIESLDDHGYLEDSLEELAAGLAGADALEEQEELVHRFTVALRLLQSLEPVGVGARNLAECLTLQLQHL
AAQDDDQTDEATVQTALRICAQPMDLLARRDVRRLMQACAASEERVRAAMALIARLEPRPGRRFADVERNLVVPDVIVRK
MARSRGDGQPQFSVQLNPEVMPRLRVHDIYANALRGHKGSEGHAALQQRLQEARWFIKNIQQRFDTILRVSQAIVQRQRN
FFTHGELAMRPLVLRDIADELGLHESTISRVTTAKYMATPMGTYELKYFFGSGLGTETGGNASSTAVRALIKQFVAAENP
LKPLSDSQLAEMLKEQGIECARRTVAKYRDVLKIAPANLRKSL

Nucleotide


Download         Length: 1572 bp        

>NTDB_id=736408 M9799_RS09410 WP_231041427.1 2102800..2104371(+) (rpoN/rpoN1) [Comamonas endophytica strain 5MLIR]
TTGAAGCCGGGTCTGTCGCTACGGGTATCGCAACACCTCGCCCTCACGCCGCAGTTGCAGCAATCGATCTGGCTGCTCCA
GCTCTCGACGCTGGAGCTGAGCCAGGAAGTCGAGCACATGCTCGACGACAACCCCTTTCTCGAACGCACCACGGAAGAAG
CCGAGCGCGAGGAATTCGGCCTGGCGCAGTCCGACGTGCCGGCGCAGCGCGACGACTATGCCGAGGATGCGCAGCATGCG
TCGGCAGAGATGTCGGGCAGCAGTGGCGATGCCGACGCCGGCGAGGTCACTGCGCCGGCCGATGCCGCGCCCGATTGGGA
AGGCGACGGCAGCAGCGATCTCGCGCCCGATGACAGCGAGTGGGGCGGCGAGGCGCGCGCGCGCAATGGCAGCAGCGAGG
ACGACGCCGAGGCCCAGGACCTGGCGCACGGCCACGAGTCGCTGACCGATCACCTGCACCGCCAGGCGCTGTCGCTCAGG
CTGGGCGAGGTCGATCGCGCGGCGCTGCGCTTTCTCATCGAGTCGCTCGATGACCATGGCTATCTGGAGGATTCGCTCGA
GGAACTGGCCGCGGGACTGGCCGGCGCGGATGCACTGGAGGAGCAGGAGGAACTGGTGCACCGCTTCACGGTGGCGCTGC
GCCTGCTGCAGTCGCTCGAGCCGGTGGGCGTGGGAGCGCGCAACCTGGCCGAATGCCTGACCCTGCAGCTGCAGCACCTC
GCGGCGCAGGACGACGACCAGACCGACGAGGCCACCGTGCAGACCGCGCTGCGCATCTGCGCGCAGCCCATGGACCTGCT
GGCGCGCCGCGACGTGCGCCGCCTGATGCAGGCCTGCGCGGCCAGCGAGGAGCGCGTGCGCGCGGCCATGGCGCTGATCG
CGCGGCTCGAGCCGCGGCCCGGGCGGCGCTTTGCCGACGTGGAGCGCAACCTGGTCGTGCCCGACGTGATCGTGCGCAAG
ATGGCGCGCAGCCGCGGCGACGGCCAGCCGCAGTTCAGCGTGCAGCTCAATCCCGAGGTGATGCCGCGGCTGCGCGTGCA
TGACATCTATGCCAACGCGCTGCGCGGCCACAAGGGCAGCGAGGGGCACGCGGCGCTGCAGCAGCGGCTGCAGGAGGCGC
GCTGGTTCATCAAGAACATCCAGCAGCGCTTCGACACCATCCTGCGCGTGTCGCAGGCCATCGTGCAGCGGCAGAGGAAC
TTCTTCACCCATGGCGAGCTGGCGATGCGCCCGCTGGTGCTGCGCGACATCGCCGACGAGCTGGGGCTGCATGAATCGAC
CATCAGCCGCGTGACCACGGCCAAGTACATGGCCACGCCGATGGGCACCTACGAGCTCAAGTATTTCTTCGGCTCGGGCC
TGGGCACGGAGACCGGCGGCAATGCCTCGAGCACGGCGGTGCGCGCGCTGATCAAGCAGTTCGTCGCGGCCGAGAATCCG
CTCAAGCCTCTCTCGGACAGCCAGCTGGCCGAAATGCTCAAGGAGCAGGGCATCGAATGCGCGCGCCGCACCGTCGCCAA
GTACCGCGACGTGCTGAAGATCGCGCCGGCCAATCTGCGCAAGTCGCTATAG

Domains

Predicted by InterproScan.

Sigma-54, DNA binding domain (Sigma54_DBD)

(365-521)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(139-350)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

53.333

100

0.535