Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   P4833_RS25850 Genome accession   NZ_CP120871
Coordinates   5469420..5470829 (-) Length   469 a.a.
NCBI ID   WP_030046895.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain PACL     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5464420..5475829
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  P4833_RS25840 (P4833_25800) - 5466514..5467692 (-) 1179 WP_028680817.1 substrate-binding domain-containing protein -
  P4833_RS25845 (P4833_25805) pilC 5468206..5469420 (-) 1215 WP_003112721.1 type II secretion system inner membrane protein GspF Machinery gene
  P4833_RS25850 (P4833_25810) pilB/pilB1 5469420..5470829 (-) 1410 WP_030046895.1 type II secretion system ATPase GspE Machinery gene
  P4833_RS25855 (P4833_25815) gspD 5470826..5473237 (-) 2412 WP_014603262.1 type II secretion system secretin GspD -
  P4833_RS25860 (P4833_25820) - 5473234..5473827 (-) 594 WP_003123065.1 type II secretion system protein M -
  P4833_RS25865 (P4833_25825) gspL 5473814..5475025 (-) 1212 WP_201268088.1 type II secretion system protein GspL -

Sequence

Protein


Download         Length: 469 a.a.        Molecular weight: 50996.40 Da        Isoelectric Point: 5.7943

>NTDB_id=734908 P4833_RS25850 WP_030046895.1 5469420..5470829(-) (pilB/pilB1) [Pseudomonas aeruginosa strain PACL]
MSLLPYAWAKAQRALLRPGEHGATLLVSPRTPGWAISEVRQRHAPASLESVRDDELDTLLASAYSDTGSAAAVVGAAESE
VDLDRLMDDIPEVTDLLDTQDGAPVIRMINALLTQAARDEASDIHIEPFETHSVVRYRVDGALRDVVAPRKALHAALVSR
IKIMAQMDIAEKRLPQDGRIALRVAGRPIDIRVSTVPTGHGERVVMRLLDKQAGRLRLETLGMAPGVLAPLDNLIRQPHG
IVLVTGPTGSGKTTTLYAALARLDASTSNILTVEDPVEYDLPGISQIQVNARIDMTFAVALRAILRQDPDIIMIGEIRDL
ETAQIAVQASLTGHLVLATLHTNDAVSAVTRLVDMGVEPFLLASSMLGVLAQRLVRRLCTHCRVEEDGGWRAVGCPACNQ
TGYSGRTGIHELFVIDDEIRRLVHQGRAEQDLREAARAAGMRSMREDGERWIASGSTTLEEILRVTRDA

Nucleotide


Download         Length: 1410 bp        

>NTDB_id=734908 P4833_RS25850 WP_030046895.1 5469420..5470829(-) (pilB/pilB1) [Pseudomonas aeruginosa strain PACL]
ATGAGCCTGCTGCCCTACGCCTGGGCCAAGGCGCAGCGCGCCTTGCTGCGGCCGGGCGAGCATGGCGCGACCCTGCTGGT
CAGCCCGCGGACGCCCGGCTGGGCGATCAGCGAGGTGCGCCAGCGGCATGCGCCGGCCAGCCTGGAGAGCGTCCGCGACG
ACGAGCTCGACACCCTGCTGGCCAGCGCCTACTCCGATACCGGCAGCGCCGCGGCGGTGGTCGGCGCGGCGGAGAGCGAG
GTCGACCTCGACCGCCTGATGGACGATATCCCCGAGGTTACCGACCTGCTCGACACCCAGGATGGCGCGCCAGTGATCCG
CATGATCAACGCGCTGCTGACCCAGGCCGCGCGCGATGAGGCCAGCGACATCCACATCGAGCCCTTCGAGACCCATTCGG
TGGTGCGCTACCGCGTCGACGGCGCCCTGCGCGACGTGGTCGCGCCGCGCAAGGCGCTGCACGCGGCGCTGGTGTCGCGG
ATCAAGATCATGGCGCAGATGGATATCGCCGAGAAACGCCTGCCCCAGGACGGCCGCATCGCCTTGCGGGTAGCCGGGCG
GCCGATCGACATTCGCGTCTCCACCGTGCCCACCGGCCACGGCGAACGGGTGGTGATGCGCCTGCTGGACAAGCAGGCCG
GACGCCTGAGGCTGGAGACCCTGGGCATGGCTCCCGGCGTGCTGGCGCCGCTGGACAACCTGATCCGCCAGCCGCACGGC
ATCGTGCTGGTCACCGGGCCCACCGGCAGCGGCAAGACCACCACCCTCTACGCCGCCCTGGCGCGGCTCGACGCCAGCAC
CAGCAACATCCTCACCGTCGAGGACCCGGTGGAGTACGACCTGCCGGGGATCAGCCAGATCCAGGTCAACGCGCGCATCG
ACATGACCTTCGCCGTAGCCCTGCGGGCGATCCTCCGGCAGGACCCGGACATCATCATGATCGGCGAGATCCGCGACCTG
GAGACCGCGCAGATCGCCGTGCAGGCTTCGCTCACCGGGCACCTGGTGCTGGCCACGCTGCACACCAACGACGCGGTGTC
GGCAGTGACGCGGCTGGTGGACATGGGGGTCGAGCCGTTCCTGCTCGCCTCGTCGATGCTTGGCGTACTCGCCCAGCGCC
TGGTGCGGCGCCTGTGCACGCATTGCCGGGTCGAAGAGGACGGCGGCTGGCGCGCGGTCGGCTGCCCGGCTTGCAACCAG
ACCGGCTACAGCGGGCGCACCGGCATCCACGAACTGTTCGTGATCGACGACGAGATCCGCCGCCTGGTCCACCAGGGCCG
CGCCGAGCAGGATCTCCGCGAAGCCGCCCGCGCGGCCGGCATGCGCAGCATGCGCGAGGACGGCGAGCGCTGGATCGCCA
GCGGCTCCACCACCCTGGAGGAAATCCTCCGCGTGACCCGGGACGCCTGA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(111-377)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

41.328

99.574

0.412
  pilB Vibrio cholerae strain A1552

45.283

90.405

0.409
  pilF Thermus thermophilus HB27

50.663

80.384

0.407
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

48.205

83.156

0.401
  pilF Neisseria gonorrhoeae MS11

46.954

84.009

0.394
  pilB Acinetobacter baumannii D1279779

41.629

94.243

0.392
  pilB Vibrio campbellii strain DS40M4

46.076

84.222

0.388
  pilB Vibrio parahaemolyticus RIMD 2210633

45.823

84.222

0.386
  pilB Acinetobacter baylyi ADP1

45.361

82.729

0.375
  pilB Legionella pneumophila strain ERS1305867

45.244

82.942

0.375