Detailed information NTG0733934

Bioinformatically predicted

Overview

• Name: rapF
• Type: Regulator
• Locus tag: P5655_RS20950
• Genome accession: NZ_CP120681
• Coordinates: 3884815..3885936 (+)
• Length: 373 a.a.
• NCBI ID: WP_003246478.1
• Uniprot ID: O34327
• Organism: Bacillus subtilis strain DSM 10
• Function: inhibit the DNA-binding function of ComA (predicted from homology) Competence regulation

Genomic Context

Location: 3879815..3890936

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
P5655_RS20925 (P5655_20925)	ycdA	3879823..3880887 (-)	1065	WP_003234750.1	DUF4352 domain-containing protein	-
P5655_RS20930 (P5655_20930)	-	3880910..3881041 (-)	132	WP_003246334.1	hypothetical protein	-
P5655_RS20935 (P5655_20935)	ycdB	3881215..3882633 (+)	1419	WP_003246352.1	YcdB/YcdC domain-containing protein	-
P5655_RS20940 (P5655_20940)	ycdC	3882820..3884181 (+)	1362	WP_010886397.1	YcdB/YcdC domain-containing protein	-
P5655_RS20945 (P5655_20945)	cwlK	3884189..3884692 (-)	504	WP_009966473.1	peptidoglycan L-alanyl-D-glutamate endopeptidase CwlK	-
P5655_RS20950 (P5655_20950)	rapF	3884815..3885936 (+)	1122	WP_003246478.1	response regulator aspartate phosphatase RapJ	Regulator
P5655_RS20955 (P5655_20955)	ycdF	3886043..3886819 (+)	777	WP_003246415.1	SDR family oxidoreductase	-
P5655_RS20960 (P5655_20960)	ycdG	3886844..3888529 (+)	1686	WP_003234736.1	alpha-glucosidase	-
P5655_RS20965 (P5655_20965)	znuA	3888717..3889676 (+)	960	WP_003234733.1	metal ABC transporter substrate-binding protein	-
P5655_RS20970 (P5655_20970)	znuC	3889732..3890427 (+)	696	WP_003234730.1	zinc ABC transporter ATP-binding protein ZnuC	-

Sequence

Protein

• Length: 373 a.a.
• Molecular weight: 44414.31 Da
• Isoelectric Point: 4.8371

>NTDB_id=733934 P5655_RS20950 WP_003246478.1 3884815..3885936(+) (rapF) [Bacillus subtilis strain DSM 10]
MRAKIPSEEVAVKLNEWYKLIRAFEADQAEALKQEIEYDLEDMEENQDLLLYFSLMEFRHRIMLDKLMPVKDSDTKPPFS
DMLNEIESNQQKLTGLLEYYFYYFRGMYEFKQKNFILAIDHYKHAEEKLEYVEDEIEKAEFLFKVAEVYYHIKQTYFSMN
YASQALDIYTKYELYGRRRVQCEFIIAGNLTDVYHHEKALTHLCSALEHARQLEEAYMIAAAYYNVGHCKYSLGDYKEAE
GYFKTAAAIFEEHNFQQAVQAVFSLTHIYCKEGKYDKAVEAYDRGIKSAAEWEDDMYLTKFRLIHELYLGSGDLNVLTEC
FDLLESRQLLADAEDLLHDTAERFNQLEHYESAAFFYRRLMNIKKKLAEQRFQ

Nucleotide

• Length: 1122 bp

>NTDB_id=733934 P5655_RS20950 WP_003246478.1 3884815..3885936(+) (rapF) [Bacillus subtilis strain DSM 10]
ATGAGAGCAAAGATTCCATCAGAAGAAGTAGCAGTAAAGCTGAATGAATGGTACAAGCTCATTCGCGCATTTGAAGCAGA
TCAAGCAGAAGCGTTAAAGCAGGAGATTGAATATGATTTAGAAGATATGGAAGAGAATCAGGACTTGCTTTTATATTTTT
CTTTGATGGAATTCCGGCATCGAATCATGCTTGATAAGCTGATGCCGGTGAAAGACAGCGATACCAAGCCTCCGTTCTCT
GATATGCTGAACGAAATTGAAAGCAATCAGCAAAAACTCACGGGCTTACTGGAATACTACTTTTATTATTTCAGAGGAAT
GTACGAATTCAAGCAGAAAAATTTCATATTAGCGATTGACCATTATAAACATGCCGAGGAAAAGCTCGAGTATGTCGAGG
ATGAGATCGAAAAAGCTGAGTTTCTTTTTAAAGTCGCAGAAGTGTATTATCACATCAAACAAACGTATTTTTCCATGAAT
TATGCGAGCCAGGCGCTTGATATCTATACGAAATATGAACTGTACGGTCGCCGCCGGGTTCAGTGTGAATTTATCATCGC
CGGAAATTTGACCGATGTGTATCATCATGAAAAAGCACTGACCCACTTGTGCAGCGCTTTAGAACATGCCAGGCAGCTTG
AAGAAGCGTACATGATCGCTGCTGCCTATTATAATGTCGGACACTGTAAGTACAGTCTGGGTGATTACAAAGAAGCGGAG
GGCTATTTCAAAACAGCCGCCGCCATTTTTGAGGAGCACAACTTTCAGCAAGCTGTTCAAGCTGTATTTTCACTGACCCA
TATCTACTGTAAAGAGGGAAAATACGACAAGGCAGTAGAGGCTTATGACCGCGGGATAAAGAGCGCGGCTGAATGGGAGG
ATGACATGTACTTGACGAAATTCCGCCTCATTCATGAGCTGTATCTTGGAAGCGGCGACCTGAACGTGCTTACAGAATGC
TTTGACCTGCTGGAATCCAGGCAGCTTCTTGCTGACGCCGAAGATCTGCTGCATGATACAGCGGAACGTTTCAATCAGTT
GGAACATTACGAGTCCGCCGCTTTCTTTTACCGCAGGCTGATGAATATTAAAAAGAAGCTTGCCGAGCAGCGTTTTCAAT
AG

Domains

Predicted by InterProScan.

• response regulator aspartate phosphatase H, N terminal (RapH_N) (4-65)

• Tetratricopeptide repeat (TPR_12) (196-252)

3D structure

• PDB: 4GYO

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
rapF	Bacillus subtilis subsp. subtilis str. 168	42.391	98.66	0.418
rapC	Bacillus subtilis subsp. subtilis str. 168	41.257	98.123	0.405