Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   P5657_RS11420 Genome accession   NZ_CP120621
Coordinates   2193914..2194324 (+) Length   136 a.a.
NCBI ID   WP_085186522.1    Uniprot ID   -
Organism   Bacillus subtilis strain DSM 13019     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 2188914..2199324
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  P5657_RS11400 (P5657_11400) - 2190876..2191496 (+) 621 WP_277709880.1 hypothetical protein -
  P5657_RS11405 (P5657_11405) - 2191744..2191922 (+) 179 Protein_2193 hypothetical protein -
  P5657_RS11410 (P5657_11410) spoIVCA 2191880..2193340 (+) 1461 WP_241496901.1 site-specific DNA recombinase SpoIVCA -
  P5657_RS11415 (P5657_11415) - 2193371..2193718 (-) 348 Protein_2195 sigma-70 family RNA polymerase sigma factor -
  P5657_RS11420 (P5657_11420) nucA/comI 2193914..2194324 (+) 411 WP_085186522.1 sporulation-specific Dnase NucB Machinery gene
  P5657_RS11425 (P5657_11425) yqeB 2194356..2195078 (-) 723 WP_072175806.1 hypothetical protein -
  P5657_RS11430 (P5657_11430) gnd 2195330..2196223 (+) 894 WP_046160611.1 phosphogluconate dehydrogenase (NAD(+)-dependent, decarboxylating) -
  P5657_RS11435 (P5657_11435) yqeD 2196243..2196869 (-) 627 WP_064671015.1 TVP38/TMEM64 family protein -
  P5657_RS11440 (P5657_11440) cwlH 2197056..2197808 (+) 753 WP_134991322.1 N-acetylmuramoyl-L-alanine amidase CwlH -
  P5657_RS11445 (P5657_11445) yqeF 2198060..2198791 (+) 732 WP_134991320.1 SGNH/GDSL hydrolase family protein -
  P5657_RS11450 (P5657_11450) - 2199097..2199237 (-) 141 WP_003226124.1 sporulation histidine kinase inhibitor Sda -

Sequence

Protein


Download         Length: 136 a.a.        Molecular weight: 14995.00 Da        Isoelectric Point: 5.1853

>NTDB_id=733286 P5657_RS11420 WP_085186522.1 2193914..2194324(+) (nucA/comI) [Bacillus subtilis strain DSM 13019]
MKKWMAGLFLAAAVLLCLMVPQQIQGASSYDKVLYFPLSRYPETGSHIRDAIAEGHPDICTIDRDGADKRREESLKGIPT
KPGYDRDEWPMAVCEEGGAGADVRYVTPSDNRGAGSWVGNQMNSYPDGTRVLFIVQ

Nucleotide


Download         Length: 411 bp        

>NTDB_id=733286 P5657_RS11420 WP_085186522.1 2193914..2194324(+) (nucA/comI) [Bacillus subtilis strain DSM 13019]
ATGAAAAAATGGATGGCAGGCCTGTTTCTTGCTGCAGCAGTTCTTCTTTGTTTAATGGTTCCGCAACAGATCCAAGGCGC
ATCTTCGTATGACAAAGTGTTATATTTTCCGCTGTCTCGTTATCCGGAAACCGGCAGTCATATTAGAGATGCAATTGCAG
AGGGACATCCAGATATTTGTACCATTGACAGAGATGGAGCAGACAAAAGGCGGGAGGAATCTTTAAAGGGAATCCCGACC
AAGCCGGGCTATGACCGGGATGAGTGGCCGATGGCGGTCTGCGAGGAAGGCGGCGCAGGGGCTGATGTCCGATATGTGAC
GCCTTCTGATAATCGCGGCGCCGGCTCGTGGGTAGGGAATCAAATGAACAGCTATCCTGACGGTACCAGAGTGCTGTTTA
TTGTGCAGTAA

Domains

Predicted by InterProScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(40-131)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

62.609

84.559

0.529