Detailed information    

insolico Bioinformatically predicted

Overview

Name   rapF   Type   Regulator
Locus tag   P5649_RS02010 Genome accession   NZ_CP120613
Coordinates   403064..404239 (-) Length   391 a.a.
NCBI ID   WP_009966637.1    Uniprot ID   P96649
Organism   Bacillus subtilis strain DSM 23521     
Function   inhibit the DNA-binding function of ComA (predicted from homology)   
Competence regulation

Genomic Context

Location: 398064..409239
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  P5649_RS01980 (P5649_01980) yddQ 398386..398928 (-) 543 WP_003234256.1 cysteine hydrolase family protein -
  P5649_RS01985 (P5649_01985) lrpB 399043..399493 (+) 451 Protein_381 transcriptional regulator LrpB -
  P5649_RS01990 (P5649_01990) lrpA 399616..400026 (-) 411 WP_003246601.1 transcriptional regulator LrpA -
  P5649_RS01995 (P5649_01995) yddN 400286..401305 (+) 1020 WP_003234259.1 LLM class flavin-dependent oxidoreductase -
  P5649_RS02000 (P5649_02000) yddM 401894..402835 (-) 942 WP_010886416.1 helicase -
  P5649_RS02005 (P5649_02005) phrI 402988..403107 (-) 120 WP_010886415.1 phosphatase RapI inhibitor PhrI -
  P5649_RS02010 (P5649_02010) rapF 403064..404239 (-) 1176 WP_009966637.1 response regulator aspartate phosphatase RapI Regulator
  P5649_RS02015 (P5649_02015) spbK 404579..405379 (+) 801 WP_009966636.1 toll/interleukin-1 receptor domain-containing protein -
  P5649_RS02020 (P5649_02020) conJ 405570..405950 (-) 381 WP_009969670.1 DUF4467 domain-containing protein -
  P5649_RS02025 (P5649_02025) - 406013..406519 (-) 507 WP_009966634.1 hypothetical protein -
  P5649_RS02030 (P5649_02030) cwlT 406534..407523 (-) 990 WP_009966633.1 bifunctional lytic transglycosylase/C40 family peptidase -

Sequence

Protein


Download         Length: 391 a.a.        Molecular weight: 46566.15 Da        Isoelectric Point: 4.9688

>NTDB_id=733067 P5649_RS02010 WP_009966637.1 403064..404239(-) (rapF) [Bacillus subtilis strain DSM 23521]
MRGVFLDKDKIPYDLVTKKLNEWYTSIKNDQVEQAEIIKTEVEKELLNMEENQDALLYYQLLEFRHEIMLSYMKSKEIED
LNNAYETIKEIEKQGQLTGMLEYYFYFFKGMYEFRRKELISAISAYRIAESKLSEVEDEIEKAEFFFKVSYVYYYMKQTY
FSMNYANRALKIFREYEEYAVQTVRCQFIVAGNLIDSLEYERALEQFLKSLEISKESNIEHLIAMSHMNIGICYDELKEY
KKASQHLILALEIFEKSKHSFLTKTLFTLTYVEAKQQNYNVALIYFRKGRFIADKSDDKEYSAKFKILEGLFFSDGETQL
IKNAFSYLASRKMFADVENFSIEVADYFHEQGNLMLSNEYYRMSIEARRKIKKGEIIDENQPDSIGSSDFK

Nucleotide


Download         Length: 1176 bp        

>NTDB_id=733067 P5649_RS02010 WP_009966637.1 403064..404239(-) (rapF) [Bacillus subtilis strain DSM 23521]
TTGCGGGGTGTTTTCTTAGATAAAGATAAAATTCCGTACGACTTAGTCACGAAAAAGTTAAATGAATGGTATACATCAAT
AAAAAATGATCAAGTTGAGCAAGCCGAGATTATAAAAACAGAAGTAGAGAAAGAATTGTTAAACATGGAAGAAAATCAAG
ATGCCCTGTTATATTATCAACTATTAGAATTTAGACATGAGATAATGCTGAGTTATATGAAATCTAAGGAAATAGAAGAT
CTCAATAATGCTTATGAGACTATAAAAGAAATTGAGAAGCAAGGGCAATTAACTGGCATGTTGGAATACTATTTTTACTT
TTTTAAGGGTATGTACGAGTTTAGGCGTAAAGAATTAATTTCAGCGATAAGTGCTTATCGAATAGCTGAATCAAAGTTGT
CAGAAGTTGAGGATGAAATAGAGAAAGCAGAGTTTTTTTTCAAAGTGTCCTATGTATATTATTATATGAAACAAACATAC
TTCTCCATGAATTATGCAAATCGTGCACTCAAAATATTTAGAGAGTATGAAGAATATGCTGTCCAGACTGTGCGTTGTCA
ATTTATTGTAGCAGGAAACTTGATCGATTCATTGGAATATGAAAGAGCCTTGGAACAATTTTTGAAGTCTTTGGAAATTT
CCAAGGAAAGTAACATAGAGCATTTAATTGCAATGTCACATATGAATATTGGGATTTGTTATGATGAATTGAAAGAATAT
AAGAAGGCTTCACAACATTTAATTTTAGCGTTAGAAATTTTTGAAAAATCAAAACATAGTTTCTTAACAAAGACTTTATT
CACTCTAACCTATGTAGAAGCAAAACAACAAAATTATAATGTTGCTTTGATATACTTTAGGAAAGGGCGATTTATTGCCG
ATAAAAGTGATGATAAGGAATACTCAGCGAAATTCAAAATATTAGAGGGATTATTTTTTTCTGATGGTGAGACTCAATTA
ATAAAGAATGCATTTTCATATCTGGCTTCGAGAAAAATGTTTGCTGATGTTGAAAATTTTTCGATTGAAGTCGCTGATTA
TTTTCATGAACAAGGAAATTTAATGCTCTCTAATGAATATTATCGTATGAGTATTGAAGCAAGACGAAAAATTAAAAAAG
GGGAGATTATTGATGAAAATCAGCCGGATTCTATTGGCAGCAGTGATTTTAAGTAG

Domains

Predicted by InterProScan.

response regulator aspartate phosphatase H, N terminal (RapH_N)

(10-70)

Tetratricopeptide repeat (TPR_12)

(199-256)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  PDB 4I1A

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rapF Bacillus subtilis subsp. subtilis str. 168

38.525

93.606

0.361
  rapC Bacillus subtilis subsp. subtilis str. 168

38.108

94.629

0.361